Cd3 apc cy7 17a2

Manufactured by BioLegend

Sourced in United States

CD3 APC-Cy7 (17A2) is a fluorescently-labeled antibody product used for the detection and analysis of CD3-positive cells by flow cytometry. The APC-Cy7 fluorophore is conjugated to the 17A2 monoclonal antibody, which specifically binds to the CD3 surface antigen found on T cells.

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3 protocols using cd3 apc cy7 17a2

Murine Immune Cell Profiling Protocol

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The reagents were purchased as follows: red blood cell lysis buffer from Sigma-Aldrich (St. Louis, MO, United States); Percoll, phosphate-buffered saline (PBS) and fetal bovine serum (FBS) from Fisher Scientific; fixation solution from Biolegend.
The following anti-mouse monoclonal antibodies were purchased from BioLegend (San Diego, CA, United States): CD3 APC-Cy7 (17A2), CD4 Brilliant Violet 605 (GK1.5), CD8a PE-Cy7 (53-6.7), CD19 FITC (6D5), TCRγδ PE (GL3), and F4/80 Brilliant Violet 510 (BM8). The following anti-mouse monoclonal antibodies were purchased from BD Biosciences (San Diego, CA, United States): CD11c APC-R700 (N418). The following anti-mouse monoclonal antibodies were purchased from Bio-Rad (Hercules, CA, United States): Ly6B.2 Alexa Fluor 647 (7/4). The following anti-mouse monoclonal antibodies were purchased from Thermo Fisher Scientific: Live/Dead Fix Blue (L23105).

Rouse M.D., Stanbro J., Roman J.A., Lipinski M.A., Jacobs A., Biswas B., Regeimbal J., Henry M., Stockelman M.G, & Simons M.P. (2020). Impact of Frequent Administration of Bacteriophage on Therapeutic Efficacy in an A. baumannii Mouse Wound Infection Model. Frontiers in Microbiology, 11, 414.

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Multiparameter Flow Cytometry Immunophenotyping

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For the detection of immune cells, the following antibodies were used: CD4-BV421 (RM4-5; Biolegend, San Diego, CA, USA), CD45-BV510 (30-F11; Biolegend), CD25-AF488 (PC61; Biolegend), CD8-PerCPCy5.5 (53-6.7; Biolegend), CD11b-PE-Cy7 (M1/70; Biolegend), CD3-APC-Cy7 (17A2; Biolegend), Ly6C-PE (HK1.4; Biolegend), Ly6G-APC (1A8; Biolegend), CD45R-AF488 (RA3-6B2; BD Bioscience), CCR2-PE (475,301; R&D), CCR5-APC (HM-CCR5; Biolegend), and Gr1-APC-Cy7 (RB6-8C5, Biolegend). Cells were incubated with Fc Block (2.4G2; BD Biosciences) for 15 min prior to staining with fluorescently labeled antibodies for 30 min on ice. Flow cytometry was performed on a BD FACS Canto II (BD Biosciences) and analyzed using Flowjo software version 10.1 (Treestar Inc., Ashland, OR, USA).

Robichon K., Patel V., Connor B, & La Flamme A.C. (2020). Clozapine reduces infiltration into the CNS by targeting migration in experimental autoimmune encephalomyelitis. Journal of Neuroinflammation, 17, 53.

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Comprehensive Multiparameter Flow Cytometry

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The following anti-mouse antibodies were used for FACS: CD19-PE (6D5), CD19-BV605 (6D5), CD3-AlexaFluor700 (17A2), CD3-APCCY7 (17A2), CD4-APC-Cy7 (GK1.5), CD8-PerCP-Cy5.5 (53-6.7), CD8-FITC (5H10-1), Streptavidin BV605, PD-1-BV421 (29F.1A12), PDL-1-Pecy7 (10F.9G2), Bcl6-PE-Dazzle594 (7D1), ICOS-AlexaFluor488 (C398.4A), CD45.1- BV605 (A20), B220-APCCy7 (RA3-6B2), Live Dead Aqua 430 were from Biolegend; CD19-AlexaFluor700 (1D3), CD19-FITC (1D3), IgD-FITC (11-26c), CD8-PECy7 (53-6.7), TNF-APC (MP6-XT22), PD-L1-PE (MIH5) and were from eBioscience; CD138-BV605 (281-2), CD138-APC (281-2), CD138-PE (281-2), CD138- Biotin, CD38-BV421 (90/CD38), CXCR5-Biotin (2G8), IFN-γ-FITC (XMG1.2), Ki-67-AlexaFluor647 (B56), Blimp-1-PE-CF594 (5E7), and CD8-BUV805 (53-6.7) were from BD Biosciences.
The following anti-mouse antibodies were used for tissue immunofluorescence: B220-PE (RA3-6B2) and CD3 FITC anti-CD3 (145-2C11) from Biolegend and CD138-APC (281-2) from BD Biosciences.
HEL was conjugated with AlexaFluor647 with a protein labeling kit (Invitrogen).

Serrán M.G., Vernengo F.F., Almada L., Beccaria C.G., Gazzoni Y., Canete P.F., Roco J.A., Boari J.T., Ramello M.C., Wehrens E., Cai Y., Zuniga E.I., Montes C.L., Cockburn I.A., Rodriguez E.V., Vinuesa C.G, & Gruppi A. (2022). Extrafollicular Plasmablasts Present in the Acute Phase of Infections Express High Levels of PD-L1 and Are Able to Limit T Cell Response. Frontiers in Immunology, 13, 828734.

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