Millipore 180

Citrinin (CIT), Aflatoxin B1 (AFB1), Fumonisin B1 (FB1), Patulin (PAT), Ochratoxin A (OTA), polyethylene glycol (PEG) 1450, hypoxanthine aminopterin and thymidine (HAT) medium, hypoxanthine-thymidine (HT) medium, complete and incomplete Freund adjuvant were purchased from Sigma (USA). The mouse McAb isotyping kit was purchased from Hycult Biotechnology (Netherlands). The SP2/0 myeloma cell line was stored in our lab. The Ridascreen Fast CIT ELISA kit was purchased from the R-Biopharm group (Germany). Goat anti-mouse IgG (H+L) was purchased from BioWorld (USA). Protein-G Sepharose Fast Flow Columns were purchased from GE Healthcare (USA). Chloroauricacid (HAuCl₄) and trisodium citrate were obtained from Shanghai Chemical Reagents (Shanghai, China). The nitrocellulose membranes (Millipore 135, Millipore 180) were purchased from Millipore (USA). Othertypes of nitrocellulose membranes (Sartorius CN 140, PALL Vivid 170), conjugation pads (GL0194, Ahlstrom 8964, Ahlstrom 6613), sample pads (GL-b01, GL-b02) and absorbent pads were purchased from Jieyi Bio. Co. (China).

The HP-PRRSV strain HuN4, HP-PRRSV vaccine (HuN4-F112, TJM-F92, JXA1-R), classical PRRSV vaccine CH-1R and MLV, NADC30-like PRRSV SDHS53, NADC34-like PRRSV TZJ1341, European PRRSV (VP046 BIS and DV) and recombinant SDHS53 N protein (240 μg/ml) used in this study were retained at the HVRI of the CAAS. Porcine parvovirus (PPV), porcine circovirus type 2 (PCV-2), classical swine fever virus (CSFV), transmissible gastroenteritis virus (TGEV), porcine pseudorabies virus (PRV), and porcine epidemic diarrhea virus (PEDV) were collected at the HVRI. Latex microspheres (diameter = 300 nm) were purchased from Hangzhou Xiaoque Technology Co., Ltd. (Hangzhou, China). MES, EDC·HCl, and NHS were procured from Sigma (Sigma, St. Louis, MO, United States). Nitrocellulose (NC) membranes (Millipore 180) were obtained from Millipore (Billerica, United States). Polyester membranes were purchased from Pall Corporation (NY, United States), and absorbent paper and PVC sheets were procured from Shanghai Jinbiao Biotechnology Co., Ltd. (Shanghai, China).