Cells isolated from the lung or spleen were stained for iNKT cell subsets, NKTFH, and NKTeff, as described above. In addition to the antibodies and other reagents used for iNKT isolation described above, we also used anti-PLZF-AF647 (clone R17-809), anti-T-bet-AF488 (clone O4-46), anti-RORγt-PE-CF594 (clone Q31-378), anti-BCL-6-AF488 (clone K112-91), all from BD Biosciences, anti-KLRG1- PECy7 (clone 2F1), and anti-CTLA-4-PE (clone UC10-4B9), all from Thermo-Fisher Scientific, and anti-CX3CR1-BV711 (clone SA011F11, BioLegend). Staining for intracellular antigens, including PLZF, T-bet, RORγT and total CTLA-4 was performed by incubating in 50% Cytofix (BD Biosciences) in PBS for 15–30 minutes on ice followed by washing and staining in eBioscience permeabilization buffer (Thermo-Fisher Scientific)50 (link). Stained samples were analyzed using a Fortessa flow cytometer (BD Biosciences) and FlowJo software (Treestar).
Anti ctla 4 pe clone uc10 4b9
Manufactured by Thermo Fisher Scientific
Anti-CTLA-4-PE (clone UC10-4B9) is a laboratory reagent that detects the expression of the CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) protein. It is conjugated with the fluorescent dye Phycoerythrin (PE) for use in flow cytometry and other immunoassays.
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Lab products found in correlation
2 protocols using anti ctla 4 pe clone uc10 4b9
1
Characterization of iNKT Cell Subsets
2
Comprehensive Profiling of iNKT Cell Subsets
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Cells isolated from the lung or spleen were stained for iNKT cell subsets, NKT FH and NKT eff , as described above. In addition to the antibodies and other reagents used for iNKT isolation described above, we also used anti-PLZF-AF647 (clone R17-809), anti-T-bet-AF488 (clone O4-46), anti-RORγt-PE-CF594 (clone Q31-378), anti-BCL-6-AF488 (clone K112-91), all from BD Biosciences, anti-KLRG1-PECy7 (clone 2F1), and anti-CTLA-4-PE (clone UC10-4B9), all from Thermo-Fisher Scientific, and anti-CX3CR1-BV711 (clone SA011F11, BioLegend). Staining for total CTLA-4 expression was performed according to a using Cytofix (BD Biosciences) and eBioscience permeabilization buffer (Thermo-Fisher Scientific). Stained samples were analyzed using a Fortessa flow cytometer (BD Biosciences) and FlowJo software (Treestar).
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