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Exosome free medium

Manufactured by Umibio
Sourced in China

Exosome-free medium is a cell culture medium designed to be depleted of extracellular vesicles, including exosomes. It is intended for use in research applications where the presence of exosomes in the culture medium may interfere with experimental results.

Automatically generated - may contain errors

2 protocols using exosome free medium

1

Neutrophil Exosome Interaction Assay

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Neutrophils (5 × 105) were isolated from HC blood as described above in “Neutrophil isolation” and cultured in 500 µL exosome-free medium (Umibio, China). Exosome was isolated from the 2 mL plasma of patients or HC as described in “plasma exosome isolation” and resuspended in the PBS. After quantification by NTA, the exosome was added into each well at 2 × 1010 particles/mL.
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2

Exosome Uptake by CD4+ T Cells in Buruli Ulcer Patients

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A PKH67 Green Fluorescent Cell Linker Kit (Sigma-Aldrich) was utilized to label CD4+T cells, and a PKH26 Red Fluorescent Cell Linker Kit (Sigma-Aldrich) was utilized to label exosomes. CD4+T cells (5 × 105/well) were seeded in 12-well plate(4.5 cm2/well), cultured in 500 µL exosome-free medium (UmiBio, Shanghai, China), then activated with 1 µg/mL anti-CD3 and 1 µg/mL anti-CD28 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). Finally, they were treated with 20 µL exosomes (1 × 1010 particles/20 µL/well) for 24 hours. The uptake of labeled exosomes was detected by an inverted fluorescence microscope (LAS X3.4.7; Leica Camera, Wetzlar, Germany) and by 520-nm and 580-nm lasers. To compare the uptake of exosomes by CD4+T cells between patients with BU and the healthy controls, a PKH26 Red Fluorescent Cell Linker Kit was utilized to label exosomes and anti-human CD4-APC antibody (clone RPA-T4; BioLegend, San Diego, CA, USA) was used to stain CD4+T cells. The relative quantification of exosome uptake by CD4+T cells was evaluated by FCM analysis.
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