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Accuchek softclix pro

Manufactured by Roche
Sourced in Germany

Accu-Chek® Softclix® Pro is a lancing device used for obtaining small blood samples. It is designed to provide a comfortable and reliable blood sampling experience.

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3 protocols using accuchek softclix pro

1

Comprehensive Blood and Saliva Biomarker Profiling

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Capillary blood samples were obtained via a single-use lancet (Accu-chek Softclix Pro, Roche Diagnostics Ltd, West Sussex, UK) and microsafe collection tubes (Inverness Medical, Cheshire, UK). Test strips and a reflectance photometer (Reflotron Plus, Inverness Medical) for total cholesterol (TC), whole blood triglycerides (TAG), creatine kinase (CK) and high-density lipoproteins (HDL) were used. The low-density lipoprotein (LDL) fraction was estimated by subtracting the sum of total TAG and HDL divided by 2.19, from TC. Fasting blood glucose (FBG) was measured via whole blood samples added to a heparin and saline solution before analysis by a Biosen C-line monitor (EKF diagnostics, Ebendorfer, Germany). Salivary testosterone and CRP were measured with enzyme immunoassay kits (Salimetrics, Carlsbad, California, USA), prior to analysis on a plate reader at 450 nm (Synergy HT, BioTek Instruments, Winoski, Vermont, USA), and Gen5 microplate reader software (BioTek Instruments, Winoski, Vermont, USA).
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2

Comprehensive Anthropometric and Metabolic Assessment

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A TANITA MC-780MA P digital weight scale (TANITA Corporation, Arlington Heights, IL, USA) and a 213 SECA portable stadiometer (SECA, Hamburg, Germany) were used to carry out the weight (0.1 kg precision), body fat (0.1 kg precision), visceral fat (cm), muscle mass (0.1 kg precision), and height (0.1 cm precision) measurements. Body mass index (BMI) was calculated as weight/height squared (kg/m2). According to the WHO classification, we established the cut-off point for overweight as 24.99 kg/m2, while obesity was defined as a BMI over 30 kg/m2 and normal weight as 18.5–24.99 kg/m2.
The waist and hip circumferences were measured using a flexible measuring tape (measurement precision, 0.1 cm). To ensure accurate results, all the measurements were performed twice, and the waist–hip ratio (WHR) value was calculated as the ratio of the waist to hip circumference.
Blood pressure (systolic and diastolic) was measured using an M7 Intelli IT blood pressure monitor (OMROM, M7, Corp., Kyoto, Japan). Capillary cholesterol (mg/dl), glucose (mg/dl), and triglyceride (mg/dl) concentrations were also examined with an Accutrend®Plus instrument using two drops of blood (15–40 μl) collected from different fingers with a lancing device (Accuchek® Softclix® Pro, Roche Diagnostics GmbH, Mannheim, Germany).
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3

Anthropometric and Metabolic Measurements

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The body weights (0.1 kg precision) and heights (0.1 cm precision) of the participants were measured with them wearing light clothing and no shoes. A digital weighing scale, TANITA MC-780MA P (TANITA Corporation, Arlington Heights, IL, USA), and a SECAR portable stadiometer 213 (SECA, Hamburg, Germany) were used to carry out the measurements.
The waist and hip circumferences were measured using a flexible measuring tape (measurement precision, 0.1 cm). All the measurements were performed twice, and the mean values were calculated for data analysis.
The body mass index was calculated as weight/height squared (kg/m2), and the waist-to-hip ratio (WHR), as the ratio of the waist to hip circumference. We also examined the body fat percentage (%) and visceral fat area (cm2). BMI was interpreted according to the World Health Organization (WHO) classification. The BMI cut-off point for overweight was defined as ≥ 24 kg/m2, while obesity was defined as a BMI ≥ 30 kg/m2 [13 ]. We also examined capillary cholesterol, glucose, and TG concentrations with the Accutrend® Plus using two drops of blood (15–40 μL) collected from different fingers, by using a lancing device (Accuchek® Softclix® Pro, Roche Diagnostics GmbH, Mannheim, Germany).
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