The chemical profile of LSS was performed at 210 nm by a Shimadzu LCMS-8040 UHPLC system (Shimadzu Corp.) comprised of two LC-30AD pumps, a SIL-30AC autosampler with a CTO-30AC column oven, a DGU-20A5 degasser, and a Shimadzu CBM-20A system controller. Chromatographic analyses were achieved at 45 °C with an InertSustain C18 column (GL Science, 2.0 μM particle size, 50 mm × 2.1 mm, Shimadzu Corp.), using water-formic acid (100:0.1, v/v) and methanol as the mobile phase A and phase B, respectively. The mobile phase was delivered at a rate of 0.25 mL/min. The injection volume was 1 μL. For the gradient separation, the gradient program was processed as follows: 15%–15% B at 0–2.0 min, 15%–80% B at 2.0–5.0 min, 80%–80% B at 5.0–7.0 min, 80%–15% B at 7.0–7.5 min. The data analysis was performed using LabSolutions software (Version 5.75, Shimadzu Corp.).
Lcms 8040 uhplc system
Manufactured by Shimadzu
Sourced in Japan
The LCMS-8040 UHPLC system is a high-performance liquid chromatography and mass spectrometry instrument developed by Shimadzu. It combines ultra-high-performance liquid chromatography (UHPLC) with triple quadrupole mass spectrometry technology to provide rapid and sensitive analysis of a wide range of compounds.
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2 protocols using lcms 8040 uhplc system
1
Chemical Profiling of LSS Using UHPLC-MS
2
LCMS-based Chemical Profiling of LSE
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The chemical profile of LSE was performed at 254 nm by an LCMS-8040 UHPLC system (Shimadzu Corp., Kyoto, Japan). Chromatographic analyses were performed at 45°C with an Inert Sustain C18 column (2.0 μm particle size, 50×2.1 mm; GL Sciences, Tokyo, Japan), using water: Formic acid (100:0.1, v/v) and methanol as the mobile phase A and B, respectively. The mobile phase was delivered at a rate of 0.25 ml/min with injection volume of 1 μl. The gradient separation process was as follows: 15–15% B at 0–2.0 min, 15–80% B at 2.0–5.0 min, 80–80% B at 5.0–7.0 min, 80–15% B at 7.0–7.5 min. The data were analyzed by LabSolutions software version 5.75 (Shimadzu Corp.).
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