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Immpress anti rabbit ap

Manufactured by Vector Laboratories
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Immpress anti-rabbit-AP is an alkaline phosphatase (AP)-conjugated secondary antibody that specifically binds to rabbit primary antibodies. It is designed for use in immunohistochemistry, western blotting, and other immunoassay applications where a rabbit primary antibody is employed.

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Lab products found in correlation

Immpact vector red, by Vector Laboratories (2 mentions) Prolong gold antifade, by Thermo Fisher Scientific (2 mentions) Rabbit linker, by Agilent Technologies (2 mentions) Low ph, by Agilent Technologies (2 mentions) Anti rabbit alexa 555 secondary antibodies, by Cell Signaling Technology (2 mentions) Fluoview fv1000 confocal microscope, by Olympus (2 mentions)

2 protocols using immpress anti rabbit ap

1

Immunohistochemical and Immunofluorescence Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
IHC. Antigen retrieval was performed in low pH Retrieval Solution (Dako, Carpinteria, CA), followed by incubation with pRB antibody (1:50; overnight; 4°C), amplification with rabbit linker (Dako), and detection with Immpress anti-rabbit-AP and Immpact Vector Red (Vector Laboratories; Burlingame, CA). Slides were scanned on an AperioScope (Vista, CA). IF. Cells, plated on coverslips, were fixed (4% formaldehyde), permeabilized (0.1% triton-X), blocked (3% BSA), and incubated with Abl (mouse 8E9; 1:50; overnight, 4°C)34 (link) and BRAF (rabbit H-145; 1:500; 2h, 4°C) antibodies, followed by anti-mouse Alexa-488 and anti-rabbit Alexa-555 secondary antibodies (1:100; Cell Signaling; 1h), and mounted in ProlongGold antifade (Invitrogen). Images were captured on an Olympus Fluoview™ FV1000 Confocal microscope, 60X objective, V1.7 software, using Arg Ion (488nM excitation for Alexa-488), HeNe (543nm excitation for Alexa-555), and Diode lasers (405nm DAPI).
Jain A., Tripathi R., Turpin C.P., Wang C, & Plattner R. (2017). Abl Kinase Regulation by BRAF/ERK and Cooperation with Akt in Melanoma. Oncogene, 36(32), 4585-4596.
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2

Immunohistochemical and Immunofluorescence Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
IHC. Antigen retrieval was performed in low pH Retrieval Solution (Dako, Carpinteria, CA), followed by incubation with pRB antibody (1:50; overnight; 4°C), amplification with rabbit linker (Dako), and detection with Immpress anti-rabbit-AP and Immpact Vector Red (Vector Laboratories; Burlingame, CA). Slides were scanned on an AperioScope (Vista, CA). IF. Cells, plated on coverslips, were fixed (4% formaldehyde), permeabilized (0.1% triton-X), blocked (3% BSA), and incubated with Abl (mouse 8E9; 1:50; overnight, 4°C)34 (link) and BRAF (rabbit H-145; 1:500; 2h, 4°C) antibodies, followed by anti-mouse Alexa-488 and anti-rabbit Alexa-555 secondary antibodies (1:100; Cell Signaling; 1h), and mounted in ProlongGold antifade (Invitrogen). Images were captured on an Olympus Fluoview™ FV1000 Confocal microscope, 60X objective, V1.7 software, using Arg Ion (488nM excitation for Alexa-488), HeNe (543nm excitation for Alexa-555), and Diode lasers (405nm DAPI).
Jain A., Tripathi R., Turpin C.P., Wang C, & Plattner R. (2017). Abl Kinase Regulation by BRAF/ERK and Cooperation with Akt in Melanoma. Oncogene, 36(32), 4585-4596.
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