Peripheral blood mononuclear cells (PBMCs) were transformed into EBV B cell lines and expanded. B cells were harvested and lysed in Frackleton buffer (10 mM Tris-HCl, pH 7.5; 50 mM NaCl; 30 mM NaPPi; 50 mM NaF, 1% Triton X-100) supplemented with protease inhibitor cocktail (Sigma-Aldrich). For Western blot, 150 μg of the protein was loaded. The following antibodies were used: anti-LRBA (HPA023597, Sigma-Aldrich) and anti-CAD (11933S, Cell Signaling Technologies). Spectra™ Multicolor High Range Protein Ladder (Thermo Fisher) was used as a size marker.
Anti cad
Manufactured by Cell Signaling Technology
Anti-CAD is a laboratory reagent used for the detection and analysis of CAD (Caspase-Activated DNase) in biological samples. CAD is an enzyme involved in the DNA fragmentation process during apoptosis, or programmed cell death. Anti-CAD is a specific antibody that can be used to identify and quantify the presence of CAD in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Anti lrba, by Merck Group (1 mentions)
Spectra multicolor high range protein ladder, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Anti ps6k, by Cell Signaling Technology (1 mentions)
Anti rpl5, by Cell Signaling Technology (1 mentions)
Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti cad
1
EBV-transformed B cell Western blot
2
Multiparameter Immune Cell Profiling
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The following reagents were purchased or acquired by listed sources or companies: BD Biosciences: CD8α (53-6.7), CD44 (IM7), IL-2 (JES6-5H4), TNF-α (MP6-XT22), IFN-γ (XMG1.2), CD45RO (UCHL1), CD45RA (HI100), human CD3 (UCHT1), human IFN-γ (B27) BioLegend: CD62L (MEL-14) Cell Signaling Technologies: anti-CAD, anti pCAD (S1859, #12662S), anti-S6, anti-pS6 (S240/244, D68F8), anti S6K, anti-pS6K (T389, #9205S), anti-RPL5 (#14568S), anti-RPL22 (anti-β-Actin (D6A8, #8457). eBioscience: Fixable Viability Dye eFluor 780 (65-0865-14), eBioscience Brand Fixation/Permeabilization set (88-8824-00). GE Healthcare: Ficoll Paque Peprotech: Recombinant IL-2, IL-7, and IL-15 cytokines ThermoFisher: anti-RPL22 (# PA5-97192), PRIME Flow® #CPF-204 Assay ID VP47VTP "5 Prime ETS" bDNA Alexa Fluor 647 Stimulatory anti-CD3 (2C11) and anti-CD28 (37.51) purified in-house from hybridoma supernatants
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