Lactel® 50:50 PLGA was purchased from Durect Corporation (Cupertino, CA). Fetal bovine serum (FBS), granulocyte macrophage-colony stimulating factor (GM-CSF) recombinant mouse protein, alpha minimum essential medium, trypsin/EDTA, Alexa Fluor® 647 hydrazide, and tris(triethylammonium) salt were purchased from Life Technologies Corporation (Grand Island, NY). Lipids, including 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt) ((DSPE-PEG(2000)) amine), cholesterol, and 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). Poly (vinyl alcohol) (PVA, MW 89,000–98,000), dichloromethane (DCM), rhodamine B (Rhod B), and BSA were purchased from Sigma-Aldrich Inc. (Saint Louis, MO). 1-Ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) was purchased from Thermo Fisher Scientific Inc. (Rockford, IL). JAWSII (ATCC® CRL-11904™) immature dendritic cells were purchased from ATCC (Manassas, VA). All other chemicals were of analytical grade.
Rhodamine b rhod b
Manufactured by Merck Group
Sourced in United Kingdom
Rhodamine B (Rhod B) is a fluorescent dye widely used in various laboratory applications. It is a red-colored, water-soluble compound that exhibits strong fluorescence properties when exposed to light of a specific wavelength. Rhod B is commonly used as a tracer, a labeling agent, and in fluorescence-based analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Lactel 50 50 plga, by Durect (1 mentions)
Alpha minimum essential medium, by Thermo Fisher Scientific (1 mentions)
Alexafluor647 hydrazide, by Thermo Fisher Scientific (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Dspe peg 2000 amine, by Avanti Polar Lipids (1 mentions)
1 2 dioleoyl 3 trimethylammonium propane, by Avanti Polar Lipids (1 mentions)
2 protocols using rhodamine b rhod b
1
Lipid-based Nanoparticle Fabrication
2
Encapsulation of Model Actives in Sodium Caseinate/Chitosan Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sodium caseinate (NaCAS) from bovine milk (CAS number: 9005-46-3), low molecular weight (MW: 50-190 kDa) chitosan (CS) with a de-acetylation degree of 75-85% (CAS number: 9012-76-4) rhodamine B (RhodB), fluorescein sodium salt (FSS), dimethylphthalate (DMP), perylene (PE), sodium azide (NaN3) and acetic acid (≥99.7% purity), were all obtained from Sigma-Aldrich (UK) and used without further purification. RhodB or FSS, used as model Active 1 (hydrophilic), were encapsulated within the sodium caseinate/chitosan (NaCAS/CS) complexes. DMP or PE were employed as model Active 2 (hydrophobic), the former for release experiments while the latter for confocal microstructure visualisation purposes only. The oil phase used in all cases for emulsion preparations was commercially available sunflower oil. Water used throughout this study was passed through a double-distillation column equipped with a de-ionisation unit. Concentrations of all material are given as percentages of the weight of the specific substance over the total weight of the system it is contained within (i.e. dispersion, emulsion, etc.) and are denoted as 'wt.%'.
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