Mouse monoclonal anti human cd31 antibody

The AcSDKP was a gift from Dr. Omata from Asabio Bio Technology (Osaka, Japan). Imidapril (ACE-I) and TA-606 (ARB) were provided by Mitsubishi Tanabe Pharma (Osaka Japan) through an MTA agreement. The mouse monoclonal anti-human CD31 antibody was purchased from R&D Systems (Minneapolis, MN, USA), and the rat polyclonal anti-mouse CD31 antibody was purchased from EMFRET Analytics GmbH & Co. KG (Eibelstadt, Germany). The polyclonal rabbit anti-αSMA antibody was obtained from Gene Tex (Irvine, CA, USA). The rabbit polyclonal anti-SM22α antibody and monoclonal anti-VE-cadherin antibody were obtained from Novus Biological (Littleton, CO, USA). The polyclonal anti-GAPDH and anti-TGF-β-receptor I antibodies were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fluorescein-, rhodamine-, and Alexa 647-conjugated secondary antibodies were obtained from Jackson ImmunoResearch (West Grove, PA, USA). Antifibroblast growth factor (FGF) receptor, anti-phospho-FGF receptor, and the HRP-conjugated secondary antibodies for Western blot detection were purchased from Cell Signaling Technology (Danvers, MA, USA). TGF-β2, tumor necrosis factor (TNF)-α, and interleukin (IL)-1β were purchased from PeproTech (Rocky Hill, NJ, USA).

The AcSDKP was a gift from Dr. Omata (Asabio Bio Technology, Osaka, Japan). Imidapril (ACEi) and TA-606 (ARB) were provided (Mitsubishi Tanabe Pharma, Osaka, Japan) through an MTA. Linagliptin was provided by Boehringer Ingelheim (Ingelheim, Ingelheim am Rhein, Germany), with a MTA. The mouse monoclonal anti-human CD31 antibody (R&D Systems, Minneapolis, MN, USA) and the rat polyclonal anti-mouse CD31 antibody (EMFRET Analytics GmbH & Co. KG, Eibelstadt, Germany) were purchased. The polyclonal rabbit anti-αSMA antibody was obtained (Gene Tex, Irvine, CA, USA). The anti-TGF-β-receptor I, DPP-4 and p-smad3 antibodies were purchased (Sigma-Aldrich, St. Louis, MO, USA). Fluorescein-, rhodamine-, and Alexa 647-conjugated secondary antibodies were obtained (Jackson ImmunoResearch, West Grove, PA, USA). Anti-fibroblast specific proteins (FSP-1) and the HRP-conjugated secondary antibodies for Western blot detection were purchased (Cell Signaling Technology, Danvers, MA, USA). TGFβ2 were purchased (PeproTech, Rocky Hill, NJ, USA).