The P-mix was composed of equal volumes of 2,6-dimethylpyrazine, 2,3-diethyl-5-methylpyrazine and 2,3,5-trimethylpyrazine (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan). In the present study, 2,3-diethyl-5-methylpyrazine was used as a P-mix constituent instead of 3-ethyl-2,5-dimethyl pyrazine, which was previously included in a pyrazine cocktail to induce avoidance and fear-related behaviors in mice and deer (Osada et al., 2013 ; 2014 (link)). A large amount of 2,3-diethyl-5-methylpyrazine was contained in wolf urine (#21 in Table 1; Osada et al., 2013 ). Exposure to 2,3-diethyl-5-methylpyrazine alone induces freezing in mice (Osada et al., 2017 (link)). The time spent immobilized by mice after exposure to P-mix was similar to that to the previous one (data not shown). The O-mix was composed of equal volumes of linalool, R(+)-limonene and i-amyl acetate (Wako, Japan). These odorants have been used in studies of the rat olfactory system (Xu et al., 2000 (link); Briand et al., 2000 (link); Clarin et al., 2010 (link)).
2 6 dimethylpyrazine
Manufactured by Tokyo Chemical Industry
Sourced in Japan
2,6-dimethylpyrazine is an organic compound with the molecular formula C6H8N2. It is a colorless or pale yellow liquid with a characteristic pyridine-like odor. The compound is used as a starting material in the synthesis of various pharmaceutical and agrochemical products.
Automatically generated - may contain errors
Lab products found in correlation
Linalool, by Fujifilm (1 mentions)
R limonene, by Fujifilm (1 mentions)
Furfuryl alcohol, by Tokyo Chemical Industry (1 mentions)
Succinic acid, by Merck Group (1 mentions)
Dl malic acid, by Merck Group (1 mentions)
1 methylpyrazole, by Merck Group (1 mentions)
2 acetylfuran, by Tokyo Chemical Industry (1 mentions)
2 5 dimethylpyrazine, by Tokyo Chemical Industry (1 mentions)
L tartaric acid, by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
Hplc grade ethyl acetate, by Avantor (1 mentions)
O phenylenediamine, by Merck Group (1 mentions)
Hplc grade water, by Avantor (1 mentions)
C7 c40n alkane standard, by Merck Group (1 mentions)
Sodium chloride (nacl), by Samchun (1 mentions)
2 protocols using 2 6 dimethylpyrazine
1
Pyrazine and Odorant Mixtures for Behavioral Studies
2
Analysis of Green Coffee Volatiles and Dicarbonyls
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Green coffee beans (C. arabica Sitio Chapada from Brazil and C. robusta from Vietnam) were purchased from Trini Coffee (Goyang, Korea). DL-Malic acid (≥98%), citric acid (≥98%), L-(+)-tartaric acid (≥99.5%), and succinic acid (≥99%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Green coffee beans and organic acid powder were stored at room temperature.
For the analysis of volatile flavor compounds, C7 − C40 n-alkane standard and a divinylbenzene/Carboxen/Polydimethylsiloxane (50 μm DVB/CAR/PDMS) solid-phase microextraction (SPME) fiber were purchased from Supelco, Inc. (Bellefonte, PA, USA). Hexane, high-performance liquid chromatography (HPLC)-grade water, and HPLC-grade ethyl acetate were purchased from J.T. Baker (Philipsburg, NJ, USA). Sodium chloride (NaCl) was purchased from Samchun (Seoul, Korea). Quinoxaline, 2-methyl pyrazine, 2,5-dimethyl pyrazine, 2,6-dimethyl pyrazine, ethyl pyrazine, 2-ethyl-6-methyl pyrazine, furfural, 2-acetyl furan, furfuryl acetate, 5-methyl furfural, and furfuryl alcohol were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).
For the analysis of α-DCs, MGO (purity 40%), DA (purity 97%), o-phenylenediamine, and 1-methylpyrazole were purchased from Sigma-Aldrich. GO (purity 39%) was purchased from Tokyo Chemical Industry Co., Ltd. HPLC-grade water and HPLC-grade ethyl acetate were purchased from J.T. Baker.
For the analysis of volatile flavor compounds, C7 − C40 n-alkane standard and a divinylbenzene/Carboxen/Polydimethylsiloxane (50 μm DVB/CAR/PDMS) solid-phase microextraction (SPME) fiber were purchased from Supelco, Inc. (Bellefonte, PA, USA). Hexane, high-performance liquid chromatography (HPLC)-grade water, and HPLC-grade ethyl acetate were purchased from J.T. Baker (Philipsburg, NJ, USA). Sodium chloride (NaCl) was purchased from Samchun (Seoul, Korea). Quinoxaline, 2-methyl pyrazine, 2,5-dimethyl pyrazine, 2,6-dimethyl pyrazine, ethyl pyrazine, 2-ethyl-6-methyl pyrazine, furfural, 2-acetyl furan, furfuryl acetate, 5-methyl furfural, and furfuryl alcohol were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).
For the analysis of α-DCs, MGO (purity 40%), DA (purity 97%), o-phenylenediamine, and 1-methylpyrazole were purchased from Sigma-Aldrich. GO (purity 39%) was purchased from Tokyo Chemical Industry Co., Ltd. HPLC-grade water and HPLC-grade ethyl acetate were purchased from J.T. Baker.
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