Milli q iq 7003 05 purification system

The anti-melatonin antibody Prospect 6C was kindly provided by Dr. Andrew Foldes (Division of Animal Production, CSIRO, Blacktown, Australia), 3H-melatonin was purchased from PerkinElmer (Waltham, MA, USA), gelatin from Merck (Billerica, MA, USA), and toluene from POCH, Gliwice Poland). Chemicals of the highest purity were purchased from J. T. Baker Chemicals (Center Valley, PA, USA) and used to prepare the mobile phases: sodium acetate, sodium dihydrogen phosphate, disodium EDTA, 1-octanesulfonic acid sodium salt, citric acid, acetic acid, and phosphoric acid. Methanol and acetonitrile, both of gradient-grade HPLC purities, were purchased from Merck Millipore (Billerica, MA, USA). 5-HTOL was provided by Santa Cruz Biotechnology (Dallas, TX, USA). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ultrapure water (18.2 MΩ, TOC ≤ 3 ppb) was freshly prepared using a Milli-Q^® IQ 7003/05 purification system (Merck Millipore, Billerica, MA, USA) and was used in all procedures.

Methanol of gradient-grade high pressure liquid chromatography (HPLC) purity, ammonium iron (II) sulfate hexahydrate, and perchloric acid were provided by Merck Millipore (Billerica, MA, USA). Sodium acetate, sodium dihydrogen phosphate, sodium monohydrated phosphate, disodium EDTA, and acetic acid were purchased from J. T. Baker Chemicals (Center Valley, PA, USA). Tetrahydrobiopterin, dithiothreitol, 3-hydroxybenzylhydrazine dihydrochloride (NSD-1015), catalase from bovine liver, TRIS base, pyridoxal 5’-phosphate hydrate, pargyline, 5-tryptamine, acetyl-CoA sodium salt, S-adenosylmethionine, TRP, 5-HTRP, 5-HT, NAS, MLT, 5-HIAA, 5-MIAA, 5-MTOL, and 5 MTAM were obtained from Sigma-Aldrich (St. Louis, MO, USA). 5-HTOL and 5-MTRP were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Ultrapure water (18.2 MΩ, TOC ≤ 3 ppb), which was freshly prepared using a Milli-Q^® IQ 7003/05 purification system (Merck Millipore, Billerica, MA, USA), was used in all procedures.