Anti p70s6k antibody

Manufactured by Abcam

The Anti-p70S6K antibody is a research tool designed to detect and quantify the p70S6K protein. p70S6K is a serine/threonine-protein kinase that plays a key role in the regulation of cell growth and proliferation. This antibody can be used in various immunoassay techniques to measure p70S6K levels in biological samples.

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Lab products found in correlation

Anti flag antibody, by Merck Group (2 mentions) Anti mtor antibody, by Cell Signaling Technology (2 mentions) Anti gfp antibody, by Santa Cruz Biotechnology (2 mentions) Anti gapdh antibody, by Proteintech (2 mentions) Horseradish peroxidase conjugated sheep anti mouse and anti rabbit antibodies, by Jackson ImmunoResearch (2 mentions) Anti lamp2 antibody, by Santa Cruz Biotechnology (2 mentions) Anti gapdh antibody, by Merck Group (1 mentions) Anti lc3b antibody, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Hoechst, by Merck Group (1 mentions) Anti lc3b antibody, by Novus Biologicals (1 mentions) Anti becn1 antibody, by Santa Cruz Biotechnology (1 mentions) Anti tubulin antibody, by Proteintech (1 mentions) Alexa fluor 594 conjugated affinipure donkey anti mouse igg antibody, by Proteintech (1 mentions) Anti ha antibody, by Santa Cruz Biotechnology (1 mentions) Anti tfeb antibody, by Cell Signaling Technology (1 mentions) Alexa fluor 488 conjugated goat anti rabbit igg, by Proteintech (1 mentions) Alexa fluor 594 conjugated goat anti mouse igg, by Proteintech (1 mentions) Anti α tubulin, by Proteintech (1 mentions) Alexa fluor 594 conjugated goat anti rabbit igg, by Proteintech (1 mentions)

Spelling variants of this product

P70S6K (18 citations) Anti-p70S6K (9 citations)

2 protocols using anti p70s6k antibody

Antibody Panel for Protein Analysis

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The following primary antibodies were used: anti-FLAG antibody (Sigma), anti-GFP antibody (Santa Cruz), anti-GAPDH antibody (Millipore), anti-phospho-p70S6K (T389) antibody (Cell Signaling Technology), anti-p70S6K antibody (Epitomics), anti-mTOR antibody (Cell Signaling Technology), anti-LC3B antibody (Novus Biologicals), anti-LC3B antibody (Abcam), anti-GAPDH antibody (Proteintech), anti-Tubulin antibody (Proteintech), anti-MAP2 antibody (Proteintech), anti-LAMP2 antibody (Santa Cruz), anti-Bcl2 (total, pS70 or pS87) antibody (Santa Cruz) and anti-BECN1 antibody (Santa Cruz). The following secondary antibodies were used: horseradish peroxidase-conjugated sheep anti-mouse and anti-rabbit antibodies (Jackson ImmunoResearch Laboratories) for immunoblotting and Alexa Fluor 594-conjugated AffiniPure Donkey Anti-Mouse IgG antibody (Proteintech), Alexa Fluor 594-conjugated AffiniPure Donkey Anti-Rabbit IgG antibody (Proteintech) or Alexa Fluor 647-conjugated AffiniPure Donkey Anti-Rabbit IgG antibody (Yasen Biotechnology) for immunocytochemistry and immunohistochemistry. DAPI (Sigma) or Hoechst (Sigma) were used for nuclei staining.

Xu S., Ma Q., Shen J., Li N., Sun S., Wang N., Chen Y., Dong C., Tam K.Y., Prehn J.H., Wang H, & Ying Z. (2024). ALS-linked C9orf72 dipeptide repeats inhibit starvation-induced autophagy through modulating BCL2–BECN1 interaction. Acta Pharmaceutica Sinica. B, 14(5), 2026-2038.

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Comprehensive Antibody Validation Protocol

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The following primary antibodies were used in our experiments: anti‐GAPDH antibody (Proteintech), anti‐Histone H3 antibody (Proteintech), anti‐phospho‐p70S6K antibody (T389) (Cell Signaling Technology), anti‐p70S6K antibody (Epitomics), anti‐LAMP1 antibody (Abcam), anti‐LAMP2 antibody (Santa Cruz), anti‐TFEB antibody (Cell Signaling Technology), anti‐p62 antibody (Enzo Life Sciences), anti‐mTOR antibody (Cell Signaling Technology), anti‐HA antibody (Santa Cruz), anti‐FLAG antibody (Sigma), anti‐GFP antibody (Santa Cruz), anti‐Rag B antibody (Cell Signaling Technology), anti‐raptor antibody (Cell Signaling Technology), anti‐α‐Tubulin (Proteintech), and anti‐C9orf72 (Santa Cruz). The following secondary antibodies were used: horseradish peroxidase‐conjugated sheep anti‐mouse and anti‐rabbit antibodies (Jackson ImmunoResearch Laboratories). The following fluorescent secondary antibodies were used: Alexa Fluor 594‐conjugated goat anti‐rabbit IgG (Proteintech), Alexa Fluor 594‐conjugated goat anti‐mouse IgG (Proteintech), Alexa Fluor 488‐conjugated goat anti‐rabbit IgG (Proteintech), Alexa Fluor 488‐conjugated Goat anti‐mouse IgG (Proteintech), Alexa Fluor 660‐conjugated goat anti‐mouse IgG (H + L) highly cross‐adsorbed antibody (Invitrogen), and Alexa Fluor 405‐conjugated goat anti‐rabbit IgG (H + L) secondary antibody (Invitrogen).

Wang M., Wang H., Tao Z., Xia Q., Hao Z., Prehn J.H., Zhen X., Wang G, & Ying Z. (2020). C9orf72 associates with inactive Rag GTPases and regulates mTORC1‐mediated autophagosomal and lysosomal biogenesis. Aging Cell, 19(4), e13126.

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