Cd68 rabbit polyclonal antibody

The monoclonal antibody to human QTRT1 was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Monoclonal antibodies of β-catenin and E-cadherin from BD Transduction (San Jose, CA, USA) and β-actin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Monoclonal antibodies of PCNA and CD11b (Integrin αM) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Claudin-5 and claudin-2 mouse monoclonal antibodies and ZO-1 rabbit monoclonal antibody were purchased from Thermo Fisher Scientific (Rockford, IL, USA). CD68 rabbit polyclonal antibody was purchased from Abcam (Cambridge, MA, USA). All chemicals were purchased from Sigma-Aldrich unless otherwise stated. The breast cancer cell lines, including MCF7 and MDA-MB-231, were provided by Dr. Tao Pan’s lab at the University of Chicago.

Five-micron sections were deparaffinized and rehydrated in the following order for histological and immunohistochemical staining: three washes of xylene, 100% ethanol, 90% ethanol, 70% ethanol, and two washes of Tris-buffered vehicle. Slides were stained with hematoxylin and eosin for evaluation of morphology and picrosirius red to detect fibrillar collagen. Immunohistochemistry was performed using rat anti-mouse CD45 antibody (BD Biosciences), rabbit polyclonal CD68 antibody (Abcam), rabbit anti-mouse vimentin antibody (Cell Signaling), rabbit anti-mouse α-smooth muscle actin antibody (Abcam), anti-pan keratin antibody (Abcam), rabbit anti-collagen I antibody (Abcam), rabbit anti-collagen III antibody (Abcam), rabbit anti-FBN antibody (Abcam), and goat anti-mouse DCN antibody (R&D Systems, Minneapolis, MN, USA) as described previously^{10 (link)}. Histological evaluation was performed independently by two experimental pathologists who were blinded to the experimental conditions.