Fox1nu nu

1 x 10⁶ Pt45.P1 cells were implanted into the pancreata of athymic nude mice (Fox1^nu/nu; Jackson 002019) and given 10 days to engraft, following which IV administration of hRabMab1, hIgG isotype control, or vehicle began at 18 mg/kg every other day (total of 22 treatment cycles). Mice were then euthanized via cardiac puncture while sedated with isoflurane. Whole blood was collected and plasma specimens harvested as detailed above. Tumors were measured with calipers and sectioned into 2 halves, one of which was snap frozen in liquid nitrogen and the other fixed in PBS with 10% formalin. Antibody concentrations in plasma were measured as detailed above.

University of Cincinnati’s Institutional Animal Care and Use Committee approved all animal studies. Antibody co-implantation studies: Pt45.P1 cells were preincubated with the indicated antibody diluted in PBS to a concentration of 5 mg/mL for 30 minutes prior to surgery. Human IgG1 isotype control (BioXCell, BE0297) was utilized as a negative control. 1x10⁶ cells were implanted with antibody into the pancreata of athymic nude mice. Pre-formed orthotopic tumor studies: 1x10⁶ Pt45.P1 cells were implanted into the pancreata of athymic nude mice (Fox1^nu/nu; Jackson 002019). Mice were randomized into control and treatment groups. Tumor growth was followed for 7 weeks.