Bradford assay method

Fresh frozen hippocampal tissue specimens from mice and humans are stored at -80°C until utilized for RNA extraction using peqGOLD TriFast TM (peqGOLD, Germany) according to the manufacturer's instructions to obtain RNA and proteins. The RNA pellet was extracted from the aqueous phase, air-dried, resuspended in 25μl of nuclease-free water (Promega). The concentration of the RNA samples was measured using the Qubit 3.0 Fluorometer (High sensitivity, Invitrogen) and stored at -80°C until further use. Proteins were extracted from the organic phase, cleaned with Ethanol, and the dried pellet resuspended with a 150 μl buffer containing 8M Urea in 4% (w/v) CHAPS and protease inhibitor (1:100 Carl Roth, Germany). The protein concentrations were determined using the Bradford assay method (Roth, Germany) and later the samples were stored until use at -80°C.

Fresh frozen hippocampal tissue specimens from mice and humans are stored at -80°C until utilized for RNA extraction using peqGOLD TriFast TM (peqGOLD, Germany) according to the manufacturer's instructions to obtain RNA and proteins. The RNA pellet was extracted from the aqueous phase, air-dried, resuspended in 25μl of nuclease-free water (Promega). The concentration of the RNA samples was measured using the Qubit 3.0 Fluorometer (High sensitivity, Invitrogen) and stored at -80°C until further use. Proteins were extracted from the organic phase, cleaned with Ethanol, and the dried pellet resuspended with a 150 μl buffer containing 8M Urea in 4% (w/v) CHAPS and protease inhibitor (1:100 Carl Roth, Germany). The protein concentrations were determined using the Bradford assay method (Roth, Germany) and later the samples were stored until use at -80°C.