Griffonia simplicifolia lectin 1 isolectin b4 dylight 649

Brain tissue was fixed in 4% paraformaldehyde for 24 h at 4°C and then stored in PBS with 0.1% NaN₃. Next, the brain was embedded in 4% ultrapure low melting point agarose (16520050, Invitrogen) in PBS. The samples were cut into 40 µm coronal sections. Free-floating sections were stained with Lycopersicon Esculentum (Tomato) Lectin DyLight^TM 488 (1:1000, L32470, ThermoFisher). Mouse cardiac and liver tissue were fixed in 4% paraformaldehyde for 24 h at 4°C, paraffin embedded and cut into 4 µm (cardiac) or 7 µm (liver) sections. Cardiac sections were stained with Griffonia Simplicifolia Lectin I Isolectin B4 Dylight^TM 649 (1:100, DL-1208, Vector Laboratories). Liver sections were stained with primary Mouse endoglin CD105 (1:100, AF1320, R&D Systems) and secondary Cyanine 3 (Cy3 1:50, NEL704A001KT, Perkin Elmer) antibody. The eyes of the mouse were enucleated and fixed in 4% paraformaldehyde for 20 min at room temperature and then washed with PBS. The retina was dissected from the eye and further fixed in 4% paraformaldehyde for 24 h at 4°C. The free-floating retinas were stained with Griffonia Simplicifolia Lectin I Isolectin B4 Dylight^TM 649 (1:100, DL-1208, Vector Laboratories) and Collagen IV polyclonal antibody (1:200, 2150–1470, Bio-Rad) with secondary Rabbit IgG (1:400, A-31572, ThermoFisher).