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NEDD1 is a protein involved in the regulation of cell division. It plays a critical role in the assembly and organization of the mitotic spindle, an essential structure for chromosome segregation during cell division. As a key component of the gamma-tubulin ring complex, NEDD1 helps recruit and anchor gamma-tubulin to the centrosome, which is crucial for microtubule nucleation and spindle formation.

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Lab products found in correlation

Prolong gold antifade mountant with dapi, by Thermo Fisher Scientific (2 mentions) A11008, by Thermo Fisher Scientific (2 mentions) α tubulin t5168, by Merck Group (2 mentions) Pericentrin, by Merck Group (2 mentions) Sp5 up, by Leica (2 mentions) Hsc70 adi spa 815, by Enzo Life Sciences (2 mentions) A11004, by Thermo Fisher Scientific (2 mentions)

2 protocols using nedd1

1

Immunofluorescence Staining of Cell Organelles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were grown in chamber slides (lab-Tek II). After inhibitor treatment, the cells were fixed with −20 °C methanol for 5 min, followed by treatment with ice-cold acetone for 30 s. After washing with PBS containing 0.1% Triton X-100, the cells were blocked with 3% BSA in washing buffer for 1 h. Primary antibodies were prepared in blocking buffer and added into the chambers for 1 h incubation at room temperature. After three washes, the cells were incubated with Alexa Fluor 488 or Alexa Fluor 568 conjugated secondary antibodies (Life technologies, A-11004 (RRID:AB_2534072), A-11008 (RRID:AB_143165),1:2000) for 1 h. After washing, cells were mounted with ProLong Gold Antifade Mountant with DAPI (Life Technologies) and visualized under a confocal microscope (Leica, SP5-Up). The primary antibodies used in this study were: HSC70 (ADI-SPA-815, RRID:AB_10617277, 1:250) from Enzo; HSP90α (ab2928, RRID:AB_303423, 1:500) from Abcam; NuMA (ABE1361, RRID:AB_2892052, 1:100) from EMD Millipore; α-tubulin (T5168, RRID:AB_477579, 1:250) from Sigma-Aldrich; Pericentrin (ab4448, RRID:AB_304461, 1:100), NEDD1 (ab57336, RRID:AB_944385, 1:100) from Cell Signaling.
Rodina A., Xu C., Digwal C.S., Joshi S., Patel Y., Santhaseela A.R., Bay S., Merugu S., Alam A., Yan P., Yang C., Roychowdhury T., Panchal P., Shrestha L., Kang Y., Sharma S., Almodovar J., Corben A., Alpaugh M.L., Modi S., Guzman M.L., Fei T., Taldone T., Ginsberg S.D., Erdjument-Bromage H., Neubert T.A., Manova-Todorova K., Tsou M.F., Young J.C., Wang T, & Chiosis G. (2023). Systems-level analyses of protein-protein interaction network dysfunctions via epichaperomics identify cancer-specific mechanisms of stress adaptation. Nature Communications, 14, 3742.
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2

Immunofluorescence Staining of Cell Organelles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were grown in chamber slides (lab-Tek II). After inhibitor treatment, the cells were fixed with −20 °C methanol for 5 min, followed by treatment with ice-cold acetone for 30 s. After washing with PBS containing 0.1% Triton X-100, the cells were blocked with 3% BSA in washing buffer for 1 h. Primary antibodies were prepared in blocking buffer and added into the chambers for 1 h incubation at room temperature. After three washes, the cells were incubated with Alexa Fluor 488 or Alexa Fluor 568 conjugated secondary antibodies (Life technologies, A-11004 (RRID:AB_2534072), A-11008 (RRID:AB_143165),1:2000) for 1 h. After washing, cells were mounted with ProLong Gold Antifade Mountant with DAPI (Life Technologies) and visualized under a confocal microscope (Leica, SP5-Up). The primary antibodies used in this study were: HSC70 (ADI-SPA-815, RRID:AB_10617277, 1:250) from Enzo; HSP90α (ab2928, RRID:AB_303423, 1:500) from Abcam; NuMA (ABE1361, RRID:AB_2892052, 1:100) from EMD Millipore; α-tubulin (T5168, RRID:AB_477579, 1:250) from Sigma-Aldrich; Pericentrin (ab4448, RRID:AB_304461, 1:100), NEDD1 (ab57336, RRID:AB_944385, 1:100) from Cell Signaling.
Rodina A., Xu C., Digwal C.S., Joshi S., Patel Y., Santhaseela A.R., Bay S., Merugu S., Alam A., Yan P., Yang C., Roychowdhury T., Panchal P., Shrestha L., Kang Y., Sharma S., Almodovar J., Corben A., Alpaugh M.L., Modi S., Guzman M.L., Fei T., Taldone T., Ginsberg S.D., Erdjument-Bromage H., Neubert T.A., Manova-Todorova K., Tsou M.F., Young J.C., Wang T, & Chiosis G. (2023). Systems-level analyses of protein-protein interaction network dysfunctions via epichaperomics identify cancer-specific mechanisms of stress adaptation. Nature Communications, 14, 3742.
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