Polyclonal rabbit anti zo 1 antibody

Immunofluorescence was performed using frozen sections (4 μm), as previously described [24 (link)]. The following antibodies were used as primary antibodies: (1) monoclonal rat anti-CD31 antibody (BD Pharmingen, San Diego, CA); (2) polyclonal rabbit anti-type IV collagen antibody (Merck Millipore, Darmstadt, Germany); (3) monoclonal anti-β-galactosidase antibody (Promega, Madison, WI); (4) monoclonal rabbit anti-PDGFRβ antibody (Cell Signaling Technology, Danvers, MA); (5) polyclonal rabbit anti-ZO-1 antibody (Zymed, Carlsbad, CA). Alexa Fluor 488 or 546 (Thermo Fischer Scientific, Waltham, MA) was used as secondary antibodies. In double immunofluorescence, images were obtained using confocal laser microscopy (LSM780; Carl Zeiss, Oberkochen, Germany) in the Central Research Laboratory, Okayama University Medical School.

Immunohistochemistry was performed using frozen sections as described previously [25] (link), [26] (link). The following antibodies were used as primary antibodies: (1) polyclonal rabbit anti-type IV collagen antibody (Chemicon International, Inc., Temecula, CA); (2) polyclonal guinea pig anti-nephrin antibody (Fitzgerald, Concord, MA); (3) polyclonal rabbit anti-ZO-1 antibody (ZYMED Laboratories, Carlsbad, CA) and (4) monoclonal rat anti-CD31 antibody (Pharmingen, San Diego, CA). The glomerular accumulation of monocytes/macrophages was determined by immunohistochemistry using monoclonal rat anti-Mac-2 (lectin, galactoside-binding, soluble, 3) antibody (Cedarlane, Burlington, Ontario, Canada) as previously described [30] (link).
Double immunofluorescent staining was performed as previously described [10] (link), [11] (link). The following antibodies were used as primary antibodies: (1) polyclonal rabbit anti-phosphorylated NF-κB p65 (pNF-κB p65) antibody (Cell Signaling Technology, Danvers, MA); (2) monoclonal rat anti-CD34 antibody (Santa Cruz Biotechnology, CA).