Nanosep 30k omega centrifugal devices

Manufactured by Pall Corporation

Sourced in United States

The Nanosep 30K Omega Centrifugal Devices are laboratory equipment used for the separation and concentration of macromolecules and particles. The devices feature a 30,000 molecular weight cut-off (MWCO) membrane and are designed for use in centrifugation processes.

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Lab products found in correlation

Iodoacetamide, by Merck Group (3 mentions) Formic acid, by Merck Group (3 mentions) Dithiothreitol (dtt), by Merck Group (3 mentions) Benzamidine hydrochloride hydrate, by Merck Group (2 mentions) Hplc grade acetonitrile and solvents a, by Merck Group (2 mentions) Guanidine hydrochloride gdnhcl, by Merck Group (2 mentions) Anhydrous sodium acetate naac, by Merck Group (2 mentions) Trypsin gold ms grade, by Promega (2 mentions) 6 aminocaproic acid, by Merck Group (2 mentions) N ethylmaleimide, by Merck Group (2 mentions) Ammonium bicarbonate, by Merck Group (2 mentions) Pierce quantitative colorimetric peptide assay, by Thermo Fisher Scientific (1 mentions) Milli q apparatus, by Merck Group (1 mentions) Acetonitrile acn, by Merck Group (1 mentions) Ammonium acetate, by Merck Group (1 mentions) Calcium chloride, by Merck Group (1 mentions)

Close spelling variants of this product

Nanosep centrifugal device Nanosep Omega Centrifugal device Nanosep

3 protocols using nanosep 30k omega centrifugal devices

Quantitative Proteomic Sample Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

N-Ethylmaleimide, 6-aminocaproic acid, benzamidine hydrochloride hydrate, dithiothreitol (DTT), iodoacetamide, ammonium bicarbonate (AMBIC), formic acid, and HPLC grade acetonitrile and Solvents A (0.1% formic acid) and B (0.1% formic acid in acetonitrile) for liquid chromatography-mass spectrometry (LC-MS) were from Sigma–Aldrich (St. Louis MO, USA). Guanidine hydrochloride (GdnHCl) and anhydrous sodium acetate (NaAc) were from Merck (Darmstadt, Germany). Trypsin Gold (MS grade) was purchased from Promega (Madison WI, USA). The Pierce Quantitative Colorimetric Peptide Assay and SOLAμ™ Solid Phase Extraction (SPE) HRP (horse radish peroxidase) 2mg/1 ml 96-well plates were from Thermo Fisher Scientific (Rockford IL, USA), and Nanosep® 30K Omega Centrifugal Devices were from Pall Life Sciences (Ann Arbor MI, USA).

Folkesson E., Turkiewicz A., Ali N., Rydén M., Hughes H.V., Tjörnstrand J., Önnerfjord P, & Englund M. (2020). Proteomic comparison of osteoarthritic and reference human menisci using data-independent acquisition mass spectrometry. Osteoarthritis and Cartilage, 28(8), 1092-1101.

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Quantitative Proteomic Sample Preparation

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N-Ethylmaleimide, 6-aminocaproic acid, benzamidine hydrochloride hydrate, dithiothreitol (DTT), iodoacetamide, ammonium bicarbonate (AMBIC), formic acid, and high-performance liquid chromatography (HPLC) grade acetonitrile and Solvents A (0.1% formic acid in water) and B (0.1% formic acid in acetonitrile) for LC-MS were purchased from Sigma-Aldrich (St. Louis). Guanidine hydrochloride (GdnHCl) and anhydrous sodium acetate (NaAc) were purchased from Merck (Darmstadt, Germany). Trypsin Gold (MS grade) was purchased from Promega (Madison, WI). The water used in this study was purified using a MilliQ apparatus (Millipore, Billerica, MA). The Pierce Quantitative Colorimetric Peptide Assay was purchased from Thermo Fisher Scientific (Rockford) and Nanosep 30K Omega Centrifugal Devices were purchased from Pall Life Sciences (Ann Arbor). Reversed-phase C18 columns were purchased from Nest Group (Southborough, MA).

Folkesson E., Turkiewicz A., Rydén M., Hughes H.V., Ali N., Tjörnstrand J., Önnerfjord P, & Englund M. (2020). Proteomic characterization of the normal human medial meniscus body using data‐independent acquisition mass spectrometry. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 38(8), 1735-1745.

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Micro BCA Protein Assay and SPE Protocol

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The micro bicinchoninic acid protein assay kit and Nanosep 30K Omega Centrifugal Devices were purchased from Pall Life Sciences, and SOLAμ Solid Phase Extraction HRP 2 mg/1 ml 96-well plates were purchased from Thermo Fisher Scientific. Trypsin (sequencing grade) was purchased from Promega Corporation. Calcium chloride, formic acid (FA), hydrochloric acid, and ammonium acetate were purchased from Merck. DTT and iodoacetamide were purchased from Sigma-Aldrich. Acetonitrile (ACN) was purchased from Sigma-Aldrich.

Ali N., Turkiewicz A., Hughes V., Folkesson E., Tjörnstand J., Neuman P., Önnerfjord P, & Englund M. (2022). Proteomics Profiling of Human Synovial Fluid Suggests Increased Protein Interplay in Early-Osteoarthritis (OA) That Is Lost in Late-Stage OA. Molecular & Cellular Proteomics : MCP, 21(3), 100200.

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