The moderately differentiated human tongue squamous cell carcinoma derived cell line Tca8113 was obtained from the China Center for Type Culture Collection (Wuhan, China) and the stable multidrug-resistant cell line Tca8113/PYM was previously established by induction with PYM in our lab. The squamous cell carcinoma cell lines SCC-25 and CAL-27 were from American Type Culture Collection. Above cell lines were cultured in RPMI-1640 (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (Gibco) at 37°C in a humidified atmosphere containing 5% CO2. To maintain the resistance phenotype, 0.5 mg/L PYM was added to the culture media of Tca8113/PYM cells. PYM was from PYM Harbin Bolai Pharmaceutical (Harbin, China). cDDP and 5-aza-2′-dC were from Sigma-Aldrich (Steinheim, Germany). MI-12 was from Selleck Chemicals (Houston, Texas, USA). Eighty-four tongue cancer tissue specimens were obtained from patients at the Affiliated Cancer Hospital of Guangzhou Medical University between March 2000–December 2006. Overall survival was computed from the day of surgery to the day of death or of last follow-up. The study was approved by the ethics committee of the Affiliated Tumor Hospital of Guangzhou Medical University.
Tca8113
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
The TCA8113 is a laboratory equipment used for the isolation and purification of cellular components. It is a centrifuge designed to separate biological samples based on their density and sedimentation rate. The TCA8113 can be used to isolate cells, organelles, and other cellular structures from complex biological mixtures. Its core function is to provide a reliable and efficient way to separate and concentrate specific cellular components for further analysis or experimentation.
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Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (14 mentions)
Cal27, by National Collection of Authenticated Cell Cultures (10 mentions)
Tscca, by National Collection of Authenticated Cell Cultures (7 mentions)
Rpmi 1640, by Thermo Fisher Scientific (6 mentions)
Scc 9, by National Collection of Authenticated Cell Cultures (6 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions)
Cal27, by American Type Culture Collection (4 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (4 mentions)
Scc25, by American Type Culture Collection (3 mentions)
Scc15, by National Collection of Authenticated Cell Cultures (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Tca8113, by Thermo Fisher Scientific (3 mentions)
Scc25, by National Collection of Authenticated Cell Cultures (3 mentions)
Sh hoxa as3, by Genechem (3 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (3 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
27 protocols using tca8113
1
Tca8113 Cell Line Characterization
2
Tongue Squamous Cell Carcinoma Cell Lines
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The moderately differentiated human tongue squamous cell carcinoma derived cell line Tca8113 was obtained from the China Center for Type Culture Collection (Wuhan, China) and the stable PYM-resistant cell line Tca8113/PYM was previously established in our lab. The squamous cell carcinoma cell lines SCC-25 and CAL-27 were from American Type Culture Collection. Above cell lines were cultured in RPMI-1640 (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (Gibco) at 37°C in a humidified atmosphere containing 5% CO2. To maintain the resistance phenotype, 0.5mg/L PYM was added to the culture media of Tca8113/PYM cells. PYM was from PYM Harbin Bolai Pharmaceutical (Harbin, China). cDDP was from Sigma-Aldrich (Steinheim, Germany). KU-0063794 was from Selleck Chemicals (Houston, Texas, USA). Eighty-four tongue cancer tissue specimens were obtained from patients at the Affiliated Tumor Hospital of Guangzhou Medical University between March 2000–December 2006. Overall survival was computed from the day of surgery to the day of death or of last follow-up. The study was approved by the ethics committee of the Affiliated Tumor Hospital of Guangzhou Medical University.
3
Radiation Response of TSCC Cell Line
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The human TSCC cell line Tca8113 was obtained from the China Center for Type Culture Collection (Wuhan, China). Cells were cultured in RPMI-1640 medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Minghai Biochem, Lanzhou, China), 100 U/ml penicillin and 100 µg/ml streptomycin, 1% sodium pyruvate and 2 mM glutamine (all obtained from Thermo Fisher Scientific, Inc.) at 37°C with 5% CO2 and 95% humidity. Cells were seeded in 35 mm glass Petri dishes (Nunc GmbH, Wiesbaden, Germany), and cell density was adjusted to 5×105 cells/ml at 24 h prior to irradiation. The cultured cells were divided into two groups; one group of cells were treated by X-ray, while the other were treated by heavy ion beam.
4
Culturing Oral Cancer and Epithelial Cells
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CAL-27 cells were donated by Shanghai Ninth People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China. UM-1 cells were donated by the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. TCA-8113 cells were purchased from the China Center for Type Culture Collection, Wuhan, China. Human oral mucosa epithelial cells were the keratinized squamous cells from the masticatory mucosa distributed in hard palate. CAL-27, UM-1, TCA-8113 and human oral mucosa epithelial cells were cultured in Dulbecco’s modified eagle’s medium (DMEM), Dulbecco’s modified eagle medium: nutrient mixture F-12 (DMEM/F12), Roswell Park MEMorial Institute (RPMI) 1640 and minimum essential medium (MEM) (Thermo Fisher Scientific, Waltham, MA, USA), respectively, all of which were supplied with 10% fetal bovine serum (Hyclone, Logan, UT, USA).
5
Cell Line Culture for Oral Cancer Research
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Human OSCC cell line TCA8113 was purchased from China Center for Type Culture Collection, CAL27, SCC9, SCC15 and SCC25 cells were obtained from the American Type Culture Collection (ATCC). All these cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco) supplemented with 10% foetal bovine serum (FBS, HyClone) and penicillin‐streptomycin (Thermo Scientific). Normal oral epithelial cells (HOK) were purchased from BeNa Culture Collection (Beijing, China) and cultured in Minimum Essential Medium (MEM, Gibco) supplemented with 10% foetal bovine serum (FBS, HyClone) and penicillin‐streptomycin (Thermo Scientific).
6
Transfecting Human OSCC Cell Lines
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The human OSCC cell lines TCA8113, cal27, SCC4, SCC15 and SCC25 were obtained from the China Center for Type Culture Collection (Wuhan, China). These cells were grown in RPMI-1640 medium (GIBCO, Scotland, UK), supplemented with 10% fetal bovine serum (GIBCO) at 37 °C and 5% CO2 in a humidified atmosphere. For transfection, cells in the monolayer were transfected with the plasmid encoding ANG2 mRNA and ANG2-targeted short interfering RNA (siRNA; 5′-GGACAAACCUGUUGAACCAAA-3′) using FuGENE HD transfection reagent (Roche, Basel, Switzerland) according to the manufacturer's protocol. All subsequent assays were performed 24 h after transfection, except where indicated otherwise.
7
Investigating CDK5 Regulation in Cancer Cells
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The human Hep-2 and Tca8113 cell lines were purchased from the China Center for Type Culture Collection (Wuhan, China) and maintained in modified Eagle’s medium (MEM) and RPMI-1640 (Invitrogen, USA) supplemented with 10 % fetal bovine serum (Gibco, USA). WP1066 (Calbiochem, Germany) was dissolved in DMSO (Solarbo, China) for use and storage. Hep-2 and Tca8113 cells were treated with WP1066 for 48 h at a concentration of 4.5 μM. The asON was transfected by using Lipofectamine 2000 (Invitrogen, USA) at a final concentration of 300 nmol/L and 233 nmol/L, respectively, according to the manufacturer’s instructions. 48 h after transfection, cells were harvested for total RNA or miRNAs isolation, and protein extraction. The CDK5 plasmid (CDK5(BC005115)-GV141) was synthesized by Gene Chem Company, China.
8
Culturing Human Oral Cancer Cell Lines
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Cal27 cells were obtained from American Type Culture Collection (MD, USA). Tca8113 and KB cells were bought from the China Center for Type Culture Collection (Hubei, China). HN4, HN6, HN12 and HN30 cells were provided by Shanghai Ninth Hospital of Shanghai Jiaotong University School of Medicine, generously donated by Dr. Silvio Gutkind on Dec. 2011 (NIH, MD, USA), and were used in previous studies [26 (link)–29 (link)]. The Cal27 and HN series cells were maintained in DMEM, KB cells in MEM and Tca8113 cells in RPMI-1640 supplemented with 10% fetal bovine serum (FBS) in a humidified incubator containing 5% CO2 at 37°C. All of the culture media and FBS were purchased from Invitrogen (CA, USA).
9
Keratinocyte Cell Culture and TGF-β1 Treatment
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Human oral keratinocytes (HOK) and OSCC cell lines include CAL-27, Tca8113, and SCC15 (China Center for Type Culture Collection, Wuhan, China) were cultured in the special keratinocyte growth medium (Clonetics, San Diego, CA, USA) and the Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum, respectively. All cells were treated with 5 ng/ml recombinant human TGF-β1 protein (T & L biological technology, Beijing, China).
10
Culturing Tca8113 Human Tongue Squamous Cells
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The human tongue squamous cell carcinoma cell line Tca8113 was obtained from the China Center for Type Culture Collection (Shanghai, China). Cells were cultured in RPMI-1640 containing 10% fetal bovine serum (Invitrogen Life Technologies, Carlsbad, CA, USA) at 37°C in 5% CO2 and 95% humidity.
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