Anti 8 ohdg antibody

All chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA), except for monobromobimane (mBBr), purchased from Calbiochem, San Diego, CA, USA. Biotinylated horse anti-mouse IgG was bought from Vector Laboratories (Burlington, ON, Canada). Alexa Fluor 555 conjugate of streptavidin and Prolong Antifade were purchased from Molecular Probes (Eugene, OR, USA). The anti-8-OHdG antibody was purchased from StressMarq Biosciences Inc (Victoria, BC, Canada). Donkey anti-rabbit immunoglobulin IgG and goat anti-mouse IgG antibodies (both conjugated with horseradish peroxidase) were provided by Cederlane Laboratories Ltd (Hornby, Canada). Nitrocellulose membranes (pore size, 0.22 µm) were bought from Osmonics Inc (Westborough, MA, USA) and the enhanced chemiluminescence kit Lumi-Light from Roche Molecular Biochemicals (Laval, QC Canada). Immunodetection of blotted proteins was performed with radiographic films obtained from Fuji (Minami-Ashigara, Japan). Other chemicals used were of at least reagent grade.

tert-butyl hydroperoxide (tert-BHP), SDS, phosphotungstic acid, buthylated hydroxytoluene, 2-thiobarbituric acid and malonaldehyde bis(dimethyl acetal), the Bicinchoninic protein determination assay and the anti-α-tubulin were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). The following were purchased from Abcam Inc., (Cambridge, MA, USA): rabbit polyclonal anti-PRDX1, monoclonal anti-PRDX4, monoclonal anti-PRDX6, the antigenic peptide used to raise the anti-PRDX1 antibody and 8-hydroxy-deoxyguanosine (8-OHdG). The anti-8-OHdG antibody was purchased from StressMarq Biosciences Inc., (Victoria, BC, Canada). Biotinylated horse anti-mouse antibody and Horse Serum were purchased from Vector Labs. Alexa-555 fluor streptavidin (1 mg ml⁻¹ in H₂O) and ProLong Gold antifade with DAPI were purchased from Invitrogen Life Technologies (Burlington, ON, Canada). Nitrocellulose (0.22 μm pore size; Osmonics Inc., MN, USA), donkey anti-rabbit IgG and goat anti-mouse IgG, both conjugated to horseradish peroxidase (Cedarlane Laboratories Ltd., Hornby, ON, Canada), an enhanced chemiluminescence kit (Lumi-Light; Roche Molecular Biochemicals, Laval, QC, Canada) and radiographic films (Fuji, Minamiashigara, Japan) were also used for immunodetection of blotted proteins. Other chemicals used were of at least reagent grade.