Hct116 human colorectal carcinoma cells

Manufactured by American Type Culture Collection

Sourced in United States

HCT116 human colorectal carcinoma cells are a well-characterized cell line derived from a human colorectal carcinoma. The cells exhibit epithelial-like morphology and are commonly used in cancer research.

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Lab products found in correlation

Erythro 9 2 hydroxy 3 nonyl adenine ehna hydrochloride, by Bio-Techne (1 mentions) Stable isotope labeled compounds, by Cambridge Isotopes (1 mentions) Fitc conjugated isolectin b4, by Vector Laboratories (1 mentions) Dual luciferase reporter assay reagent, by Promega (1 mentions) Ribogreen, by Thermo Fisher Scientific (1 mentions) 1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dii, by Thermo Fisher Scientific (1 mentions) 1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine perchlorate did, by Thermo Fisher Scientific (1 mentions) 6 p toluidino 2 naphthalenesulfonic acid tns, by Fujifilm (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Hela human cervical carcinoma cells, by American Type Culture Collection (1 mentions) Bovine insulin, by Merck Group (1 mentions) Iec6 rat intestinal epithelial cells, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by GE Healthcare (1 mentions) Penicillin streptomycin, by GE Healthcare (1 mentions) Hepg2 hepatocellular carcinoma cells, by American Type Culture Collection (1 mentions) Ccd841 con normal colon epithelial cells, by American Type Culture Collection (1 mentions) U87mg human glioblastoma cell, by American Type Culture Collection (1 mentions)

7 protocols using hct116 human colorectal carcinoma cells

Xeroderma Pigmentosum Fibroblasts Analysis

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All chemicals and enzymes, unless otherwise specified, were purchased from Sigma-Aldrich (St. Louis, MO) or New England Biolabs (Ipswich, WA). Stable isotope-labeled compounds were obtained from Cambridge Isotope Laboratories (Cambridge, MA). Erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA) hydrochloride was from Tocris Bioscience (Ellisville, MO). GM04429 human skin fibroblasts deficient in xeroderma pigmentosum complementation group A (XPA) were kindly provided by Prof. Gerd P. Pfeifer. The HCT-116 human colorectal carcinoma cells were purchased from ATCC (Manassas, VA).

Yu Y., Wang J., Wang P, & Wang Y. (2016). Quantification of Azaserine-induced Carboxymethylated and Methylated DNA Lesions in Cells by Nanoflow Liquid Chromatography-Nanoelectrospray Ionization Tandem Mass Spectrometry Coupled with the Stable Isotope-Dilution Method. Analytical chemistry, 88(16), 8036-8042.

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Fluorescent Probes for Cell Imaging

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EverFluor TMR-X was purchased from Setareh Biotech (Eugene, OR, USA).
6-(p-Toluidino)-2-naphthalenesulfonic acid (TNS) was obtained from Wako Chemicals (Osaka, Japan). Ribogreen, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate (DiD) were obtained from Molecular Probes (Eugene, OR, USA). TRIzol reagent was obtained from Invitrogen (Carlsbad, CA, USA). Dual-Luciferase Reporter Assay Reagent was purchased from Promega Corporation (Madison, WI, USA). FITC-conjugated Isolectin B4 was purchased from Vector Laboratories (Burlingame, CA). HeLa human cervical carcinoma cells was purchased from RIKEN Cell Bank (Tsukuba, Japan). HCT116 human colorectal carcinoma cells was purchased from ATCC (Manassas, VA, USA). All siRNAs ware obtained from Hokkaido System Science Co. Ltd. (Sapporo, Japan). The sequences for the sense and antisense strands of the siRNAs used in this study are listed in Supplementary Table.

Sato Y., Matsui H., Sato R, & Harashima H. (2018). Neutralization of negative charges of siRNA results in improved safety and efficient gene silencing activity of lipid nanoparticles loaded with high levels of siRNA. Journal of controlled release : official journal of the Controlled Release Society, 284.

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Isolation and Characterization of Lagunamide D

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The dark-brown encrusting cyanobacterial tufts (DRTO 85) were collected from Loggerhead Key, Dry Tortugas, Florida in 2015 and were identified as a mixture of Dichothrix sp., Lyngbya sp. with red algae Ceramium sp. serving as scaffold, and Rivularia sp. Lagunamide D (2.58 mg, >95% purity) was isolated from 71.3 g of freezedried material as previously described. [3] (link) 2.2. Cell culture HCT116 human colorectal carcinoma cells and HeLa human cervical carcinoma cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco's Modified Eagle Medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin-streptomycin (Invitrogen) and maintained under a humidified environment with 5% CO 2 at 37 °C.

Luo D., Ratnayake R., Atanasova K.R., Paul V.J, & Luesch H. (2023). Targeted and functional genomics approaches to the mechanism of action of lagunamide D, a mitochondrial cytotoxin from marine cyanobacteria. Biochemical pharmacology, 213.

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Genetic engineering of HCT116 cells

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HCT116 human colorectal carcinoma cells were purchased from the American Type Culture Collection (Manassas, VA, USA). p53^−/−, Bax^−/− and p21^−/− HCT116 cells were obtained from Dr Bert Vogelstein (Johns Hopkins University, Baltimore, MD, USA). The pEGFP-C3-Bax expression vectors were provided by Dr Quan Cheng (Institute of Zoology, Chinese Academy of Sciences, Beijing, China).

Li S.Z., Ren J.W., Fei J., Zhang X.D, & Du R.L. (2018). Cordycepin induces Bax-dependent apoptosis in colorectal cancer cells. Molecular Medicine Reports, 19(2), 901-908.

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Cell Culture Conditions for SFA Studies

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IEC6 rat intestinal epithelial cells and HCT116 human colorectal carcinoma cells were originally purchased from American Type Culture Collection (Manassas, VA, USA). DMEM medium was supplemented with 10% (v/v) FBS and 1% (v/v) penicillin-streptomycin antibiotics (growth media) for HCT116. 0.1 unit/ml bovine insulin (Sigma Aldrich) was also added to growth media for IEC6. Cells were kept in a humidified incubator at 37 °C with 5% CO2. Cells were seeded and treated as needed 24 h after plating. Cells were grown to a final confluence of ~80%. Prior to SFA treatment cells were serum-starved for 8 h. Pre-treatments with pharmacological inhibitors were carried out 1 h prior to the addition of SFAs unless otherwise indicated.

Choi S., Snider J.M., Cariello C.P., Lambert J.M., Anderson A.K., Cowart L.A, & Snider A.J. (2020). Sphingosine kinase 1 is required for myristate-induced TNFα expression in intestinal epithelial cells. Prostaglandins & other lipid mediators, 149, 106423.

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Cytotoxicity Evaluation of Cell Lines

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All chemicals were purchased from Sigma (USA) unless stated otherwise. HCT 116 human colorectal carcinoma cells, CCD-841-CoN normal colon epithelial cells, Jurkat E6.1 lymphoblastic leukemic cells, HepG2 hepatocellular carcinoma cells, and V79-4 Chinese hamster lung fibroblasts were obtained from American Type Culture Collection (Rockville, MD USA). CCD-841-CoN and V79-4 cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM), whereas HepG2 and Jurkat E6.1 cells were maintained in Eagle Minimum Essential Medium (EMEM) and RPMI 1640 medium, respectively. All mediums were purchased from Gibco Invitrogen, USA, and supplemented with 10% fetal bovine serum (PAA Laboratories, GmbH) and 100 U/mL of Penicillin-Streptomycin (PAA Laboratories, GmbH). Cells were cultured at 37°C in 5% CO₂ condition at the Toxicology Laboratory, Faculty of Health Science, Universiti Kebangsaan Malaysia.

Leong L.M., Chan K.M., Hamid A., Latip J, & Rajab N.F. (2016). Herbal Formulation C168 Attenuates Proliferation and Induces Apoptosis in HCT 116 Human Colorectal Carcinoma Cells: Role of Oxidative Stress and DNA Damage. Evidence-based Complementary and Alternative Medicine : eCAM, 2016, 2091085.

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Cell Culture of Common Cancer Cell Lines

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The A431 human epidermoid carcinoma cells, U87MG human glioblastoma cells and HCT-116 human colorectal carcinoma cells were purchased from the American Type Culture Collection (ATCC, Rockville MD). The cells were grown in Dulbecco’s modified Eagle medium (DMEM), Minimum Essential Medium (MEM), and McCoy’s 5A Medium respectively, all of which were supplemented with 10% FBS, penicillin (100 IU/mL), and streptomycin (100 mg/mL) and cultured at 37 °C in a humidified atmosphere containing 5% CO₂. Cells were passaged three times per week.

Cheng S., Jacobson O., Zhu G., Chen Z., Liang S.H., Tian R., Yang Z., Niu G., Zhu X, & Chen X. (2018). PET Imaging of EGFR Expression Using an 18F-labeled RNA Aptamer. European journal of nuclear medicine and molecular imaging, 46(4), 948-956.

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