PCR was performed using GoTaq Green Master Mix (Promega, Madison, WI, USA) according to the manufacturer’s protocol. The sequences of the primers used for the evaluation are listed in Table S1 . PCR products were loaded on a 2% agarose gel (Agarose S: NIPPON GENE, Tokyo, Japan) with a fluorescent DNA staining reagent (Midori Green Xtra: NIPPON Genetics, Tokyo, Japan) and separated in 1× TAE buffer. Specific bands were detected by the FAS-V gel imaging system (NIPPON Genetics).
Fas 5 gel imaging system
Manufactured by Nippon Genetics
The FAS-V gel imaging system is a compact and versatile instrument designed for visualizing and documenting nucleic acid and protein gels. It utilizes a high-resolution camera and specialized lighting to capture clear, high-quality images of electrophoresis gels. The core function of the FAS-V is to provide researchers with an efficient tool for the analysis and documentation of their gel-based experiments.
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Lab products found in correlation
2 protocols using fas 5 gel imaging system
1
PCR Protocol using GoTaq Reagents
2
Splicing Efficiency Analysis by RT-PCR
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Total RNAs were isolated from HEK293T cells, and splicing efficiency was analyzed by RT-PCR using a primer set targeting skipped exon (Supplemental Table S7 ). The PCR products were separated on a 2.5% agarose gel in 0.5× TBE buffer (44.5 mM Tris-borate, 1 mM EDTA) and visualized with a FAS-V gel imaging system (Nippon Genetics Co., Ltd.). The unspliced pre-mRNA and spliced mRNA were quantified using NIH ImageJ software and normalized according to their product lengths.
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