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Mouse anti complex 5

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Mouse anti-complex V is a primary antibody that specifically recognizes the mitochondrial ATP synthase (complex V) in mouse samples. It can be used to detect and quantify the expression of complex V in various mouse tissues and cell lines.

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Lab products found in correlation

Phosphatase inhibitor cocktail 1, by Merck Group (2 mentions) Rabbit anti phosphothreonine, by Merck Group (2 mentions) Rabbit anti phosphoserine, by Merck Group (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Mouse anti dynamin, by Abcam (2 mentions) Donkey anti rabbit alexa488, by Jackson ImmunoResearch (1 mentions) Cy3 donkey anti guinea pig, by Jackson ImmunoResearch (1 mentions) Goat anti mouse igg2b alexa 488, by Thermo Fisher Scientific (1 mentions) Alexa 568 goat anti rabbit, by Thermo Fisher Scientific (1 mentions)

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Mouse anti (2 citations)

3 protocols using mouse anti complex 5

1

Immunolabeling of Larval Brains and Cells

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Wandering third instar larval brains were dissected and fixed as previously described (Sen et al., 2013 (link)) with the following modification. Larval brains were labeled sequentially using: guinea pig anti-Clu N-terminus (1:2000; Cox and Spradling, 2009 (link)), donkey anti-guinea pig Cy3 (1:500, Jackson ImmunoResearch, West Grove, PA, USA), rabbit anti-GFP (1:2000, Torrey Pines Biolabs, Secaucus, NJ, USA), and donkey anti-rabbit Alexa 488 (1:500, Jackson ImmunoResearch, West Grove, PA, USA), with 3 times, 10 min washes (1× PBS:0.1% Triton X-100:1% BSA) in between each antibody. S2R+ culture cells were fixed and mounted as described (Sen et al., 2013 (link)). The following primary antibodies were used: mouse anti-Complex V (1:1000, cat# MS507, Mitosciences, Eugene, OR, USA) and rabbit anti-GFP (Torrey Pines Biolabs). The following secondary antibodies were used: goat anti-mouse IgG2b Alexa 488, and goat anti rabbit Alexa 568 (Molecular Probes, Life Technologies, Grand Island, NY, USA). Images were collected using Zeiss 710 and Zeiss 700 confocal microscopes and 63× Plan Apo NA 1.4 lens.
Sen A, & Cox R.T. (2016). Clueless is a conserved ribonucleoprotein that binds the ribosome at the mitochondrial outer membrane. Biology Open, 5(2), 195-203.
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2

Drosophila Adult Head Proteomic Analysis

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Drosophila adult head extract was obtained by homogenizing adult heads collected on dry ice in high EDTA RIPA buffer (50 mM Tris-HCl, pH7.5, 1% NP-40, 0.5% NaDoc, 150 mM NaCl, 0.1% SDS, 10 mM EDTA, 50 mM NaF, 1 mM Na3VO4, 250 nM cycloporin A, protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail 1 (Sigma) using mortar and pestle. 10–20 μg protein homogenate was separated by SDS-PAGE and transferred to nitrocellulose membranes. Primary antibodies were diluted in blocking solution as following: rabbit anti-p-Synj, 1:5000; guinea pig anti-Endo(GP60), 1:200; rabbit anti-Dap160, 1:5000; rabbit anti-Mnb, 1:500, rabbit anti-Synj-1-rabbit. 1:200; mouse anti-dynamin, 1:200; mouse anti-complex V, 1:10,000 (MitoSciences); rabbit anti-phosphothreonine, 1:200 and rabbit anti-phosphoserine, 1:200 (EMD Millipore). To detect the amount of protein loading, nitrocellulose membrane was stained with Direct Blue 71. All values were normalized to control done within the same experimental set. Intensity of each band was quantified using Image J. Full images of key western blots are shown in Supplementary Fig. 7.
Chen C.K., Bregere C., Paluch J., Lu J., Dickman D.K, & Chang K.T. (2014). Activity-dependent facilitation of Synaptojanin and synaptic vesicle recycling by the Minibrain kinase. Nature communications, 5, 4246.
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3

Drosophila Adult Head Proteomic Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Drosophila adult head extract was obtained by homogenizing adult heads collected on dry ice in high EDTA RIPA buffer (50 mM Tris-HCl, pH7.5, 1% NP-40, 0.5% NaDoc, 150 mM NaCl, 0.1% SDS, 10 mM EDTA, 50 mM NaF, 1 mM Na3VO4, 250 nM cycloporin A, protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail 1 (Sigma) using mortar and pestle. 10–20 μg protein homogenate was separated by SDS-PAGE and transferred to nitrocellulose membranes. Primary antibodies were diluted in blocking solution as following: rabbit anti-p-Synj, 1:5000; guinea pig anti-Endo(GP60), 1:200; rabbit anti-Dap160, 1:5000; rabbit anti-Mnb, 1:500, rabbit anti-Synj-1-rabbit. 1:200; mouse anti-dynamin, 1:200; mouse anti-complex V, 1:10,000 (MitoSciences); rabbit anti-phosphothreonine, 1:200 and rabbit anti-phosphoserine, 1:200 (EMD Millipore). To detect the amount of protein loading, nitrocellulose membrane was stained with Direct Blue 71. All values were normalized to control done within the same experimental set. Intensity of each band was quantified using Image J. Full images of key western blots are shown in Supplementary Fig. 7.
Chen C.K., Bregere C., Paluch J., Lu J., Dickman D.K, & Chang K.T. (2014). Activity-dependent facilitation of Synaptojanin and synaptic vesicle recycling by the Minibrain kinase. Nature communications, 5, 4246.
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