The human EC109 cell line (esophageal squamous carcinoma) and 293T cell line were obtained from the Shanghai Cell Bank (http://www.ctcccas.ac.cn/xibao ). Cells were cultivated in DMEM supplemented with fetal bovine serum (FBS,10%) in a humidified at mosphere containing 5% CO2 in air at 37℃.
293t cell line
Manufactured by Shanghai Cell Bank
Sourced in China
The 293T cell line is a human embryonic kidney cell line commonly used in cell and molecular biology research. It is a highly transfectable cell line, which makes it a useful tool for the production and study of recombinant proteins and viral vectors. The 293T cell line expresses the SV40 large T antigen, which allows for the episomal replication of transfected plasmids containing the SV40 origin of replication.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Xav939, by Selleck Chemicals (1 mentions)
Mg132, by Selleck Chemicals (1 mentions)
Lncap, by Shanghai Cell Bank (1 mentions)
Du145, by Shanghai Cell Bank (1 mentions)
2 protocols using 293t cell line
1
Cultivation of EC109 and 293T Cell Lines
2
Investigating UBE2N in Prostate Cancer
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The human prostatic epithelial cell line (HPEpic) was obtained from XinYu Bio-Technology, Shanghai, China. Four prostate cancer cell lines 22RV1, PC3, LNCaP and DU145, and a 293T cell line were provided by the Shanghai Cell Bank (Shanghai, China). RPMI-1640 and DMEM with a mixture of 10% fetal bovine serum (Gibco, Grand Island, NY, USA), penicillin (100 units/ml) and streptomycin (100 µg/ml) were used for cell culture and the cultural condition was 37 °C and 5% CO2/95% air atmosphere. To investigate the role of UBE2N in process of Axin1 protein synthesis, PC3 cells were treated with 10 µM proteasome inhibitor MG132 (S2619; Selleck). To investigate the role of Wnt/β-catenin in UBE2N-mediated prostate cancer progression, PC3 cells were treated with 10 µM XAV939 (S1180; Selleck).
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