Sterile nontreated 96 well microplates

Lysis assays were performed in sterile nontreated 96-well microplates (Brand, Wertheim, Germany) at 37°C with shaking (400 rpm) for 2 h. Heat-inactivated cells of S. aureus strain COL and M. luteus, were prepared as above, and were resuspended in 50 mmol/L Tris pH 8.0 to an initial OD₆₀₀ of 0.3. Low-molecular weight salmon sperm DNA and purified proteins AMR_1–2 and R₃GL were added to the wells at the beginning of the assay at different concentrations. Three biological replicates were performed in triplicate in an Infinite F200 PRO microplate reader (Tecan Group Ltd., Männedorf, Switzerland).
The decrease in OD₆₀₀ observed for each protein/DNA concentrations was calculated as a percentage of the initial OD₆₀₀ value.

Strain CIP 106760 was grown in sterile non-treated 96-well microplates (Brand, Wertheim, Germany) in MH broth. Overnight cultures were diluted to an initial optical density (OD) OD_600nm of 0.05 and supplemented with AHR to final concentrations of minimum inhibitory concentration (MIC), MIC/2 and 2MIC. Cultures were grown at 37 °C with shaking (180 rpm) and monitored for 24 h in a microplate reader (Tecan Group Ltd., Männedorf, Switzerland). Three biological replicates were performed.