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Precision xs microplate sample processor

Manufactured by Agilent Technologies
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The Precision XS microplate sample processor is a compact and automated liquid handling system designed for precise and accurate transfer of small sample volumes. It features a multi-channel pipetting head and is capable of handling standard microplates. The core function of the Precision XS is to automate liquid handling tasks, enabling efficient and reproducible sample preparation and assay setup.

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Lab products found in correlation

Goat anti higg fc hrp, by Merck Group (2 mentions) El406 washer dispenser, by Agilent Technologies (2 mentions) Biostack microplate stacker, by Agilent Technologies (2 mentions) Tetracycline, by Merck Group (1 mentions) Deep well plates, by Avantor (1 mentions) Ampicillin, by Merck Group (1 mentions) 384 well microtiter plate, by Greiner (1 mentions) Prism 9, by GraphPad (1 mentions) Monolith nt automated capillary chips, by NanoTemper (1 mentions) Mo affinity analysis software, by NanoTemper (1 mentions) Protein labeling kit red nhs 2nd generation, by NanoTemper (1 mentions)

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Precision XS (6 citations)

5 protocols using precision xs microplate sample processor

1

Preparation and Storage of RNAi Library Clones

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Plates from Ahringer’s RNAi library containing the clones of interest (purchased from the Medical Research Council (MRC) GeneService) were replicated to Luria-Bertani  (LB) agar supplemented with ampicillin (100 μg/ml, Sigma-Aldrich) and tetracycline (15 μg/ml, Sigma-Aldrich) using a pin replicator (Boekel) and grown overnight at 37 °C. The day after, the selected clones were inoculated in 1.3 ml of LB supplemented with ampicillin (100 μg/ml, Sigma-Aldrich), tetracycline (15 μg/ml, Sigma-Aldrich) and 8% glycerol in deep well plates (VWR) and incubated overnight at 37 °C with shaking (180 rpm, New Brunswick™ Innova® 44/44R incubator shaker). The last column of the plates was left free for convenient control. The next day, 120 μl of the culture was transferred to microtitre plates using a Precision XS Microplate Sample Processor (Biotek, Winooski, VT, USA) and frozen at −80 °C.
Hernando-Rodríguez B., Erinjeri A.P., Rodríguez-Palero M.J., Millar V., González-Hernández S., Olmedo M., Schulze B., Baumeister R., Muñoz M.J., Askjaer P, & Artal-Sanz M. (2018). Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans. BMC Biology, 16, 36.
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2

SARS-CoV-2 Antibody Titration ELISA

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For titration ELISA, purified IgGs were titrated from 3.18 μg/mL- 0.001 ng/mL on 30 ng/well of the following antigens: S1-S2-His (High Five cell produced), RBD-mFc (High Five cell produced), S1-mFc (High Five cell produced) and TUN219-2C1-mFc (as control for unspecific Fc binding). In addition, all scFv-hFc were also tested only at the highest concentration (3.18 μg/mL) for unspecific cross-reactivity on Expi293F cell lysate (104 cells/well), BSA (1% w/v) and lysozyme. IgGs were detected using goat-anti-hIgG(Fc)-HRP (1:70000, A0170, Sigma). Titration assays were performed using 384 well or 96 well microtiter plates (Greiner Bio-One) using Precision XS microplate sample processor (BioTek), EL406 washer dispenser (BioTek) and BioStack Microplate stacker (BioTek). EC50 were calculated with by GraphPad Prism Version 6.1, fitting to a four-parameter logistic curve. Titration ELISAs on other coronaviruses and S1-HIS mutants were performed as described above.
Bertoglio F., Fühner V., Ruschig M., Heine P.A., Abassi L., Klünemann T., Rand U., Meier D., Langreder N., Steinke S., Ballmann R., Schneider K.T., Roth K.D., Kuhn P., Riese P., Schäckermann D., Korn J., Koch A., Chaudhry M.Z., Eschke K., Kim Y., Zock-Emmenthal S., Becker M., Scholz M., Moreira G.M., Wenzel E.V., Russo G., Garritsen H.S., Casu S., Gerstner A., Roth G., Adler J., Trimpert J., Hermann A., Schirrmann T., Dübel S., Frenzel A., Van den Heuvel J., Čičin-Šain L., Schubert M, & Hust M. (2021). A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations. Cell Reports, 36(4), 109433.
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3

UPEC Survival under HOCl Stress

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The survival of UTI89 and UTI89 ppk under HOCl stress in the presence and absence of various mesalamine concentrations was investigated as described previously22 (link). UPEC UTI89 WT and UTI89 ppk were grown in the presence and absence of various concentrations of mesalamine at 37 °C with shaking in MOPS glucose medium to mid-log phase. Once absorbance at 600 nm had reached 0.45, 2.5 mM HOCl was added to 125 ml baffled flasks and incubated at 37 °C with shaking (200 r.p.m.) for 30 min. Cells (0.5 ml) were collected by centrifugation immediately before and 30 min after HOCl addition, then rinsed with MOPS medium containing 10 mM Na2S2O3, but no glucose, K2HPO4 or thiamine. Dilutions in 0.9% NaCl and spot-titering on LB agar were performed using a Precision XS Microplate Sample Processor (Bio-Tek). Each strain was tested at least four times.
Dahl J.U., Gray M.J., Bazopoulou D., Beaufay F., Lempart J., Koenigsknecht M.J., Wang Y., Baker J.R., Hasler W.L., Young V.B., Sun D, & Jakob U. (2017). The anti-inflammatory drug mesalamine targets bacterial polyphosphate accumulation. Nature microbiology, 2, 16267.
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4

Quantifying SARS-CoV-2 Antibody Responses

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For titration ELISA, sera were diluted 1:100 to 1: 9.19 × 107 in 384-well microtiter plates (Greiner Bio-One) coated with 30 ng/well of the respective RBD variant. In addition, all sera were also tested at the lowest dilution (1:100) for determination of unspecific cross-reactivity on Expi293F cell lysate (30 ng/well), BSA (30 ng/well), and lysozyme (30 ng/well). IgGs in the sera were detected using goat-anti-hIgG(Fc)-HRP (1:70,000, A0170, Sigma). Three-hundred-eighty-four-well liquid handling was performed with a Precision XS microplate sample processor (BioTek), EL406 washer dispenser (BioTek), and BioStack Microplate stacker (BioTek). OD450 nm-620 nm was measured in an ELISA plate reader (BioTek Instruments, Epoch) and its software Gen5 version 3.03 was used to calculate EC50 values, further expressed as relative potency towards an internal calibrant for which the Binding Antibody Unit (BAU) was calculated using the WHO International Standard 20/136 in relation to the original Wuhan strain RBD. The graphics were created by GraphPad Prism 9.1. Significance was calculated by pairwise non-parametric multiple comparison ANOVA (Friedman’s test) with Dunn’s multiple comparisons test, using the Wuhan wt RBD values as the reference value for all three VOCs, but Omicron data were shown separately for better illustration.
Schubert M., Bertoglio F., Steinke S., Heine P.A., Ynga-Durand M.A., Maass H., Sammartino J.C., Cassaniti I., Zuo F., Du L., Korn J., Milošević M., Wenzel E.V., Krstanović F., Polten S., Pribanić-Matešić M., Brizić I., Baldanti F., Hammarström L., Dübel S., Šustić A., Marcotte H., Strengert M., Protić A., Piralla A., Pan-Hammarström Q., Čičin-Šain L, & Hust M. (2022). Human serum from SARS-CoV-2-vaccinated and COVID-19 patients shows reduced binding to the RBD of SARS-CoV-2 Omicron variant. BMC Medicine, 20, 102.
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5

ACE2-hFc Affinity Measurements Using MST

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The affinity measurements were performed as described before [23 (link)]. In brief, ACE2-hFc was labeled by the Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper) according to the manufacturer’s protocol. A degree of labeling (DOL) of < 3 was achieved and 10 nM of the labeled ACE2-hFc was applied in the measurements. Titration of the RBD variants was done by a Precision XS microplate sample processor (BioTek) in 384-well plates. Measurement was performed in Monolith (Nanotemper) using Monolith NT. Automated Capillary Chips (NanoTemper). The Excitation-Power was set to 40% and MST-Power to medium. The timeframe of 0.5 s up to 1.5 s was chosen to analyze the data by the MO Affinity Analysis software (NanoTemper) by Hill fit. For all RBD variants, a signal response above 18 and a signal to noise above 40 was obtained.
Schubert M., Bertoglio F., Steinke S., Heine P.A., Ynga-Durand M.A., Maass H., Sammartino J.C., Cassaniti I., Zuo F., Du L., Korn J., Milošević M., Wenzel E.V., Krstanović F., Polten S., Pribanić-Matešić M., Brizić I., Baldanti F., Hammarström L., Dübel S., Šustić A., Marcotte H., Strengert M., Protić A., Piralla A., Pan-Hammarström Q., Čičin-Šain L, & Hust M. (2022). Human serum from SARS-CoV-2-vaccinated and COVID-19 patients shows reduced binding to the RBD of SARS-CoV-2 Omicron variant. BMC Medicine, 20, 102.
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