HIV viral load was determined with Cepheid Xpert HIV-1 Viral Load, and a result <40 copies/mL was considered undetectable. CD4+ and CD8+ T-cell counts were determined using flow cytometry analysis of freshly collected peripheral blood (within 4–6 hours after blood sampling).
Xpert hiv 1 viral load
Manufactured by Cepheid
The Xpert HIV-1 Viral Load is a diagnostic instrument designed to quantify the level of HIV-1 virus in a patient's blood sample. It utilizes real-time PCR technology to provide accurate and reliable results for viral load measurement.
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6 protocols using xpert hiv 1 viral load
1
HIV Viral Load and T-Cell Quantification
2
Quantifying HIV RNA in Duodenal Tissue
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Duodenal tissue supernatant was obtained by concentrating 15 mL of duodenal tissue collection media using Amicon Ultra Centrifugal filters (Millipore) returning a volume of 2 mL. Plasma was collected by centrifuging whole blood to separate the plasma and red blood cells. HIV RNA levels in the duodenal lining fluid and plasma were measured by quantitative PCR. In brief, 1 mL of specimen was loaded into HIV-1 viral load assay cartridges with integrated reaction tubes (Xpert HIV-1 Viral Load, Cepheid). The cartridges were loaded into a GeneXpert instrument system which carried out RT-PCR and viral load was quantified by the instrument software. Detection level as defined by the manufacturer was 40 copies/mL.
3
Evaluation of Finger-Prick HIV Viral Load
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Four 100 µl finger-prick samples were taken from each participant as well as a 10 ml venepuncture sample which was sent to the clinical laboratory for validated pVL quantitation using the Roche TaqMan assay on the same day. The lower limit of pVL quantification was defined as < 20 copies HIV RNA/ml using venous blood plasma on the Roche TaqMan assay. Two finger-prick samples were processed on the Cepheid Xpert HIV-1 Viral Load and two on the HIV-1 Qual cartridges. Each finger-prick was taken using the provided minivette (Cepheid) which collected 100 µl of blood. For each pair of samples, one was processed neat and one following a 3 minute spin in a microcentrifuge. Details were as follows:
4
Quantitative HIV Viral Load and T-cell Counts
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HIV viral load was determined with Cepheid Xpert HIV-1 Viral Load, and a result of <40 copies/mL was considered undetectable. CD4+ and CD8+ T-cell counts were determined by flow cytometry analysis of freshly collected peripheral blood (within 4–6 hours after blood sampling).
5
Profiling HIV-Infected Youth in Africa
6
Comprehensive HIV Screening and Diagnosis
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Alere™ HIV Combo (rebranded to Determine™ HIV Ultra, from Abbott) was used for the screening of HIV infection. It is a fourth-generation rapid test that detects IgM and IgG Abs to HIV-1/HIV-2. Moreover, it detects p24 Ag to HIV-1. This is what sets it apart from third-generation testing, as it allows detecting both HIV Abs and p24 Ag with the same test [24 ].
Rapid, qualitative HIV RNA testing (Xpert® HIV-1 Qual, Cepheid) was performed to confirm reactive p24 Ag and/or HIV Ab test. If result detected HIV, a positive HIV diagnosis was confirmed. In case of divergent results, a second quantitative HIV RNA test (Xpert® HIV-1 Viral Load, Cepheid) was performed. If the second quantitative HIV RNA test result was positive, the final HIV diagnostic result was considered positive, whereas if the second test was negative, the final HIV diagnosis was negative. In parallel a blood sample was send to an external laboratory to perform a Western Blot assay (WB). Tests that produced false-positive results for the “p24 Ag only” were investigated by the manufacturer.
A rapid testing of T-lymphocyte counts (PIMATM CD4, Abbott) was also performed to assess baseline immunity.
Rapid, qualitative HIV RNA testing (Xpert® HIV-1 Qual, Cepheid) was performed to confirm reactive p24 Ag and/or HIV Ab test. If result detected HIV, a positive HIV diagnosis was confirmed. In case of divergent results, a second quantitative HIV RNA test (Xpert® HIV-1 Viral Load, Cepheid) was performed. If the second quantitative HIV RNA test result was positive, the final HIV diagnostic result was considered positive, whereas if the second test was negative, the final HIV diagnosis was negative. In parallel a blood sample was send to an external laboratory to perform a Western Blot assay (WB). Tests that produced false-positive results for the “p24 Ag only” were investigated by the manufacturer.
A rapid testing of T-lymphocyte counts (PIMATM CD4, Abbott) was also performed to assess baseline immunity.
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