Sodium alginate

Manufactured by Nacalai Tesque

Sourced in Japan

Sodium alginate is a water-soluble, linear polysaccharide derived from brown seaweed. It is a common ingredient used in various laboratory applications due to its unique chemical properties. Sodium alginate acts as a thickening, stabilizing, and gelling agent, making it a versatile component in numerous experimental procedures.

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Lab products found in correlation

Chitin, by Nacalai Tesque (1 mentions) Gellan gum, by Nacalai Tesque (1 mentions) Heparin sodium, by Nacalai Tesque (1 mentions) Chitosan, by Nacalai Tesque (1 mentions) Laminarin, by Nacalai Tesque (1 mentions) Carboxymethyl cellulose sodium salt, by Nacalai Tesque (1 mentions) Sypro orange, by Merck Group (1 mentions) L rhamnose, by Fujifilm (1 mentions) Galua, by Fujifilm (1 mentions) Dna modifying enzymes, by Toyobo (1 mentions) Restriction endonucleases, by Toyobo (1 mentions)

5 protocols using sodium alginate

Alginate Lyase-Mediated Hydrogel Preparation

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The DEH solution was prepared and quantitated as described previously^{13 (link)}, but without endo-type alginate lyase A1-I, except when stated. Briefly, an alginate solution [1% (w/v)] was prepared by autoclaving 1.0 g of sodium alginate (Nacalai Tesque) in 100 mL of pure water (Elix, Millipore). To this alginate solution, 1.0 mL of the purified exo-type alginate lyase Atu3025 (2.15 mg/mL, 8.57 U/mg), prepared as described previously^{15 (link)}, was added, and the reaction mixture was incubated at 30 °C and 100 spm for 18 h. The mixture was filtered through a Centriprep-10K centrifugal filter at 4 °C and 1,600 × g for 30 min, and then the filtrate was freeze-dried, adjusted to a concentration of 2.5% (w/v) with pure water, and sterilized using a 0.22-μm filter.
When DEH reactivity was examined, the DEH solution with other compounds to be tested was incubated at 30 °C and the reaction stopped by immersion in ice water.

Nakata S., Murata K., Hashimoto W, & Kawai S. (2019). Uncovering the reactive nature of 4-deoxy-l-erythro-5-hexoseulose uronate for the utilization of alginate, a promising marine biopolymer. Scientific Reports, 9, 17147.

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Polysaccharide Degradation Assay

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The reaction mixtures (500 μl each) were prepared with 0.01 mg/ml purified protein, 1% (w/v) soluble or insoluble polysaccharides, such as chitin (Nacalai Tesque Inc.), chitosan (from crab shell; Nacalai Tesque Inc.), curdlan, cellulose (α-cellulose; Nacalai Tesque Inc.), carboxymethyl cellulose sodium salt (Nacalai Tesque Inc.), xylan (from beechwood; Nacalai Tesque Inc.), laminarin (Nacalai Tesque Inc.), sodium alginate (500 cps), pectin (from citrus; Nacalai Tesque Inc.), heparin sodium, xanthan gum (Nacalai Tesque Inc.), or gellan gum (Nacalai Tesque Inc.), and 50 mM Tris buffer pH 7.5. These reaction mixtures were incubated at 37 °C for 12 h. Then, the reaction mixtures were centrifuged at 15,000 g for 10 min and the clear fractions were transferred to clean tubes for assays. The degradation of the polysaccharides was determined using the DNS assay^{43 (link)}. The DNS reagent consisted of 1% (w/v) DNS, 30% (w/v) potassium sodium tartrate, and 0.4M NaOH. The aliquots (100 μl) were mixed with equal volumes (100 μl) of DNS reagent, boiled for 5 min, cooled to 20 °C, and then observed for a red colour or measured at an absorbance of 525 nm^{43 (link)}.

Itoh T., Nakagawa E., Yoda M., Nakaichi A., Hibi T, & Kimoto H. (2019). Structural and biochemical characterisation of a novel alginate lyase from Paenibacillus sp. str. FPU-7. Scientific Reports, 9, 14870.

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Comprehensive Carbohydrate Characterization

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Sodium alginate (average molecular weight: 300,000) from Eisenia bicyclis and agar were purchased from Nacalai Tesque. Pectin from Citrus fruits, GalUA–GalUA and SYPRO Orange were purchased from Sigma-Aldrich. l-Rhamnose and GalUA were obtained from Wako. The pectin component RG-I was from purchased Megazyme and pectic acid from Lancaster. Restriction endonucleases and DNA-modifying enzymes were obtained from Toyobo. Oligonucleotides used in this study were synthesised by Hokkaido System Science (Table S2). All other chemicals used were analytical-grade and commercially available.

Konishi H., Hio M., Kobayashi M., Takase R, & Hashimoto W. (2020). Bacterial chemotaxis towards polysaccharide pectin by pectin-binding protein. Scientific Reports, 10, 3977.

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Crude Glycerol Utilization for Biofuel Production

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Crude glycerol used in this study was provided by the Kyoto Municipal Waste Edible Oil Fuel Production Facility (https://www.city.kyoto.lg.jp/kankyo/page/0000065549.html). It comprised 45% (w/w) glycerol, 13% (w/w) methanol, 25% (w/w) lipids, and other impurities such as 2.7% (w/w) potassium, 0.92% (w/w) water, 0.02% (w/w) nitrogen, and 0.01% (w/w) sulfur. Sodium alginate (average molecular weight: 300,000) from Eisenia bicyclis and agar were purchased from Nacalai Tesque (Kyoto, Japan). Restriction endonucleases and DNA-modifying enzymes were obtained from Toyobo (Osaka, Japan) or Takara Bio (Shiga, Japan). All other analytical-grade chemicals used herein were commercially available.

Yoshida N., Takase R., Sugahara Y., Nambu Y, & Hashimoto W. (2022). Direct production of polyhydroxybutyrate and alginate from crude glycerol by Azotobacter vinelandii using atmospheric nitrogen. Scientific Reports, 12, 8032.

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Alginate-Hyaluronic Acid Composite Membrane Fabrication

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A 1% aqueous alginate solution was prepared by dissolving sodium alginate with a viscosity of 500 cps (Nacalai, Japan) in ultrapure water for more than 1 day with stirring. A 20-mL aliquot of the alginate solution and a 0 -5 mL aliquot of a HA solution (2500 mg L -1 in 0.1 M aqueous NaOH) were pipetted in a glass Petri dish (i.d. 9 cm). After mixing the solution thoroughly, the mixture was dried in a drying oven at 60°C for 12 h. Approximately 20 -30 mL of 1 M aqueous CaCl2 was then added to the dried sample, and a membrane was formed at room temperature for 2 h. CaCl2 was rinsed out with pure water, and the membrane was subsequently incubated in pure water for more than 1 h. The membrane was peeled off and cut into 2 × 4 cm sections. To remove contaminating iron, the sections were shaken in an aqueous solution consisting of 10 mM CaCl2, 50 mM ascorbic acid and 0.1 mM 1,10phenanthroline for more than 12 h with shaking. After rinsing with pure water, the sections were used or stored as a dried form. The variation of TAM-HA is referred to as TAMm-HAn, where m and n represent the volume (mL) of the alginate solution and the HA solution added in the Petri dish, respectively. The thickness of the wet membrane was measured with a micrometer, and the thickness was determined to be 0.20 ± 0.01 mm.

Iwai H. (2018). Thin Alginate Membranes Functionalized with Humic Acids for Removing Traces of Cu(II) from Contaminated Seawater. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 34(3).

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