Atp fluorimetric assay kit

Manufactured by Merck Group

The ATP fluorimetric assay kit is a laboratory tool designed to quantify the presence and concentration of adenosine triphosphate (ATP) in samples. It utilizes a fluorescent signal to detect and measure ATP levels, providing a reliable method for researchers and scientists to analyze ATP-dependent processes and cellular energy status.

Automatically generated - may contain errors

Lab products found in correlation

Spectramax paradigm multi mode detection platform, by Molecular Devices (1 mentions)

Spelling variants of this product

ATP assay kit (67 citations) Fluorimetric assay (3 citations) Fluorimetric assay kit (2 citations) ATP colorimetric/fluorimetric assay kit (2 citations)

2 protocols using atp fluorimetric assay kit

Liver Fatty Acid Oxidation Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The ex vivo radioactive fatty acid oxidation measurements were performed by using ¹⁴C-palmitic acid following an established protocol^{49 (link)}. Before radioactive measurements, the lysis was done starting from 250 mg of liver in 0.5 ml of lysis buffer, and 50 μl of lysate in addition to 150 μl total FAO reaction buffer. The reaction is performed at 37 °C × 60′. For ATP levels assessment, a piece of liver was used and the metabolite was measured by using the ATP fluorimetric assay kit from Sigma.

Ramadori G., Ljubicic S., Ricci S., Mikropoulou D., Brenachot X., Veyrat-Durebex C., Aras E., Ioris R.M., Altirriba J., Malle E., Foell D., Vogl T, & Coppari R. (2019). S100A9 extends lifespan in insulin deficiency. Nature Communications, 10, 3545.

+ Open protocol

+ Expand

Quantifying Tissue-Specific ATP Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

The total amount of ATP has been measured in spleen, heart, liver isolated from WT, ND6 and COI mice (4–6 per group) using the ATP Fluorimetric Assay Kit (Sigma-Aldrich MAK190). The tissues were snap-frozen in liquid nitrogen to prevent the ATP consumption by enzymes. Then, the tissues (10–20 mg) were homogenized in ATP assay buffer (300–400 μl) in ice and spun at 14,000 g for 5 min at 4° C, afterward the supernatant was transferred in 10 kDa molecular weight cut off spin filter and spun at 14,000 g for 5 min at 4° C to be deproteinized. The samples were transferred to a 96 wells plate and the fluorescence (λ_ex= 535 nm, λ_em= 587 nm) was measured using a SpectraMax Paradigm Multi-Mode Detection Platform (Molecular Devices). The background was subtracted from the measurement and the amount of ATP present in the samples was determined from the standard curve.

Angelin A., Gil-de-Gómez L., Dahiya S., Jiao J., Guo L., Levine M.H., Wang Z., Quinn W., Kopinski P.K., Wang L., Akimova T., Liu Y., Bhatti T.R., Han R., Laskin B.L., Baur J.A., Blair I.A., Wallace D.C., Hancock W.W, & Beier U.H. (2017). Foxp3 reprograms T cell metabolism to function in low glucose high lactate environments. Cell metabolism, 25(6), 1282-1293.e7.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!