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Apc conjugated cd45

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APC-conjugated CD45 is a fluorescent-labeled antibody that binds to the CD45 surface antigen. CD45 is a common leukocyte antigen expressed on all hematopoietic cells. The APC (Allophycocyanin) fluorescent dye is conjugated to the CD45 antibody, allowing for the detection and identification of cells expressing CD45 using flow cytometry.

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Lab products found in correlation

Fitc conjugated epcam, by BioLegend (2 mentions) Hoechst 33342 dye, by Thermo Fisher Scientific (1 mentions) Facsaria iiu, by BD (1 mentions) Pe cy7 conjugated cd56 hcd56, by BioLegend (1 mentions) Apc cy7 conjugated cd16 3g8, by BioLegend (1 mentions)

Close spelling variants of this product

APC-conjugated anti-CD45 APC‐conjugated anti‐CD45 mAb APC/Cy7-conjugated anti-human CD45 APC conjugated anti-mouse CD45 antibody APC-conjugated anti-human CD45 antibody APC/Cy7–conjugated anti-CD45 antibody APC-conjugated anti-mouse CD45 APC-conjugated anti-CD45 antibody APC-Cy7-conjugated CD45 Allophycocyanin (APC)-conjugated anti-mouse CD45

3 protocols using apc conjugated cd45

1

Multi-Marker Leukocyte Identification

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Cells were stained with a total of 4 reagents, 1 nuclear dye (Hoechst 33342), 2 antibodies against common blood cell markers (CD31 and CD45), and 1 antibody against an epithelial cell marker (EpCAM). Incubation was done simultaneously for all reagents, with the first 12 minutes at room temperature, and an additional 33 minutes on ice. Incubation was done with 5 μg/mL of Hoechst 33342 dye (Invitrogen, Carlsbad, CA, Catalog number: H3570) and 20x dilutions of APC-conjugated CD31 (Clone: WM59, Biolegend, Inc, San Diego, CA, Catalog number: 303116), APC-conjugated CD45 (Clone: HI30, Biolegend, Inc, San Diego, CA, Catalog number: 304012), and FITC-conjugated EpCAM (Clone: 9C4, Biolegend, Inc, San Diego, CA, Catalog number: 324204) antibodies. Upon completion of the incubation, cells are washed with buffer once, before being left on ice prior to FACS processing
Ooi C.C., Mantalas G.L., Koh W., Neff N.F., Fuchigami T., Wong D.J., Wilson R.J., Park S.M., Gambhir S.S., Quake S.R, & Wang S.X. (2017). High-throughput full-length single-cell mRNA-seq of rare cells. PLoS ONE, 12(11), e0188510.
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2

Isolation of Tumor Cells from Lungs and Bones

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All analyses were conducted on a BD FACS AriaIIu cell sorter and analyzer with 405 nm, 488 nm, and 630 nm lasers and non-co-linear detectors. After transduction, successive rounds of FACS for tdTomato (PE channel) were performed until a uniform population was obtained. Unlabeled Myc-CaP cells were used as a negative control. To detect tdTomato positive tumor cells in vivo, lungs or bones (tibia, femur and lumbar vertebrae) were disrupted with a mortar and pestle and strained. The cells were labeled with DAPI and mouse PE/Cy7 conjugated Ter119 (Biolegend #116222) and APC conjugated Cd45 (Biolegend #103112) antibodies. Putative tumor cells were defined as single viable cells, negative for Ter119, and successively negative for Cd45 but positive for tdTomato.
Wang Y., Herroon M.K., Zielske S.P., Ellis L., Podgorski I., Taichman R.S, & Cackowski F.C. (2021). Use of FVB Myc-CaP cells as an immune competent, androgen receptor positive, mouse model of prostate cancer bone metastasis. Journal of Bone Oncology, 30, 100386.
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3

Multiparametric Immunophenotyping of Cells

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Cells were immunophenotyped with the following fluorescent-labeled monoclonal antibodies (mAb) against: PE-Cy7-conjugated CD56 (HCD56; BioLegend, San Diego, CA), ECD/PE-CF594-conjugated CD3 (UCHT1; Beckman Coulter, Brea, CA), APC-Cy7-conjugated CD16 (3G8; BioLegend), Pacific Blue-conjugated CD45 (HI30; BioLegend), PerCP-Cy5.5/FITC-conjugated anti-human CD107a (LAMP-1) (H4A3; BioLegend), Pacific Blue/BV421-conjugated anti-human IFN-γ (4S.B3; BioLegend), FITC/Alexa Fluor 647-conjugated TNF-α (MAb11; BioLegend), FITC/PE-conjugated CD33 (P67.6; BD Biosciences), and APC-conjugated CD45 (HI30; BioLegend), FITC-conjugated EpCAM; (BioLegend). Phenotypic acquisition of cells was performed on the LSRII (BD Biosciences) and analyzed with FlowJo software (Tree Star Inc., Ashland, OR).
Vallera D.A., Felices M., McElmurry R., McCullar V., Zhou X., Schmohl J.U., Zhang B., Lenvik A.J., Panoskaltsis-Mortari A., Verneris M.R., Tolar J., Cooley S., Weisdorf D.J., Blazar B.R, & Miller J.S. (2016). IL-15 Trispecific Killer Engagers (TriKEs) Make Natural Killer Cells Specific to CD33+ Targets While Also Inducing Persistence, In Vivo Expansion, and Enhanced Function. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(14), 3440-3450.
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