The cDNA sequences of Uv1809 and OsSRT2 were ligated with the vectors pCNG (GFP tags) and pCNF (Flag tags) and then introduced individually into Agrobacterium strain EHA105. Agrobacterium carrying the pCNG‐Uv1809 and pCNF‐OsSRT2 vectors were co‐infiltrated into N. benthamiana leaves. Total proteins were extracted from N. benthamiana leaves at 2 dpi and incubated with Anti‐Flag M2 affinity gel (Yeasen Biotech, Shanghai, China). Proteins eluted from gels were analysed by immunoblotting with anti‐GFP or anti‐Flag antibodies (ABclonal, Wuhan, China).
Anti flag m2 affinity gel
Manufactured by Yeasen
Sourced in China
Anti-Flag M2 affinity gel is a chromatography resin designed for the purification of recombinant proteins that contain a FLAG-tag. The gel consists of an agarose matrix covalently linked to the anti-FLAG M2 monoclonal antibody, which recognizes and binds to the FLAG-tag sequence. This resin can be used to capture, wash, and elute FLAG-tagged proteins from cell lysates or other sample types.
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Lab products found in correlation
2 protocols using anti flag m2 affinity gel
1
Co-expression and Co-immunoprecipitation of Uv1809 and OsSRT2
2
Co-Immunoprecipitation of Tagged Proteins
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The coding sequences of UvPr1a and OsSGT1 were cloned into the vectors pCN-GFP and pCN-Flag, respectively. pCN-UvPr1a-GFP and pCN-OsSGT1-Flag vectors were introduced into Agrobacterium strain EHA105. Agrobacterium cultures harboring each construct were resuspended in infiltration buffer to a final OD 600 of 1.6 and co-infiltrated into N. benthamiana leaves. Total proteins were extracted from N. benthamiana leaves 2 days after infiltration and incubated with Anti-Flag M2 affinity gel (Yeasen Biotech, China). Proteins eluted from gels were analyzed by immunoblotting with anti-GFP (Thermo Fisher Scientific, Waltham, MA, USA) or anti-Flag antibodies (Thermo Fisher Scientific, USA).
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