Formaldehyde for molecular biology

Acrylamide/bis-acrylamide 30% solution (37.5:1), β-mercaptoethanol (molecular biology grade), Coomassie Brilliant Blue R250 (CBB), DL-dithiothreitol (HSCH₂CH(OH)CH(OH)CH₂SH, 99%) (DTT), glycerol (HOCH₂CH(OH)CH₂OH, 86–88%), iodoacetamide (IAA, ICH₂CONH₂, 99%), sodium citrate tribasic dehydrate (HOC(COONa)(CH₂COONa)₂·2H₂O, 99%), sodium carbonate (Na₂CO₃, 99%), tris-base (NH₂C(CH₂OH)₃), trifluoroacetic acid (CF₃COOH, 99%), trypsin from bovine pancreas and the Sigma Marker wide range 6.5–200 kDa were purchased from Sigma-Aldrich. Formaldehyde for molecular biology (36.5–38% in H₂O) and sodium dodecyl sulfate (SDS, CH₃(CH₂)₁₁SO₄Na) were purchased from Panreac. Bromophenol-blue was purchased from Riedel-de Haen. Hydrogen tetrachloroaurate (III) hydrate (HAuCl₄·xH₂O) (99.9%-Au) (49% Au) at 10% w/v was purchased from Strem Chemicals. Ammoniumbicarbonate (AMBIC, NH₄HCO₃, 99.5%) and formic acid (HCOOH, 95%) were purchased from Fluka.

All reagents and solvents used were HPLC-grade or higher. Acrylamide/bis-acrylamide 30% solution (37.5:1), β-mercaptoethanol (molecular biology grade), chloroplatinic acid hexahydrate (≥37.50% Pt basis), Coomassie Brilliant Blue R250 (CBB), DL-dithiothreitol (DTT, 99%,), glycerol (86%–88%), iodoacetamide (IAA, 99%), sodium borohydride (99%), sodium citrate tribasic dihydrate (99%), sodium carbonate (99%), tris-base, trifluoroacetic acid (99%), trypsin from bovine pancreas, and the Sigma Marker wide range 6.5–200 KDa were purchased from Merck (Hohen-Brunn, Germany). Formaldehyde for molecular biology (36.5–38% in H₂O) and sodium dodecyl sulfate (SDS) were purchased from Panreac (Barcelona, Spain). Bromophenol-blue was purchased from Riedel-de Haen (Seelze, Germany). Hydrogen tetrachloroaurate (III) hydrate (99.9%-Au) (49% Au) at 10% w/v was purchased from Strem Chemicals (Newburyport, MA, USA). Ammonium bicarbonate (AMBIC, 99.5%) and formic acid (95%) were purchased from Fluka (Steinheim, Germany).