Rabbit anti bid

T cells with a >95% purity were purified using an EasySep mouse T cell enrichment kit (StemCell Technologies). T cell lysates were prepared in sample buffer (50 mM Tris-Cl [pH 6.8], 50mM 2-ME, 2% SDS, 0.2% bromophenol blue, and 10% glycerol). Antibodies used for Western blots were rabbit anti-LC3 (polyclonoal P014, MBL International), hamster anti-Bcl-2 (polyclonal, BD pharmingen), rabbit anti-Bcl-x_L (polyclonal), rabbit anti-Mcl-1 (polyclonal, Rockland Immunochemicals), rabbit anti-Bim (polyclonal, Cell Signaling), rabbit anti-Bax (polyclonal, Cell Signaling), rabbit anti-Bak (polyclonal, Cell Signaling), rabbit anti-Bid (polyclonal, Abcam), rabbit anti-PARP-1 (polyclonal, Cell Signaling), rabbit anti-COX IV (polyclonal, Cell Signaling), mouse anti-cytochrome c (7H8.2C12, BioLegend), mouse anti-α-Tubulin (B-5-1-2, Sigma) and goat anti-β-Actin (polyclonal, Santa Cruz Biotechnology). For HRP-labeled western blot, the secondary antibodies were anti-rabbit IgG-HRP, anti-mouse IgG-HRP, anti-hamster IgG-HRP and anti-goat IgG-HRP (Jackson Immunoresearch). The development of the western blot was achieved with SuperSignal West Pico Chemiluminescent substrate (Thermo Scientific). For fluorescent western blot, the secondary antibodies were anti-rabbit IgG-Alexa Fluor 680, anti-mouse IgG-Alexa Fluor 680, and anti-goat IgG-Alexa Fluor 790 (Molecular Probes, Invitrogen).