Pcdna3.1 hygro expression plasmid

Manufactured by Thermo Fisher Scientific

The PcDNA3.1(+)/hygro expression plasmid is a vector designed for recombinant protein expression in mammalian cell lines. It contains elements necessary for high-level expression of the gene of interest, as well as a hygromycin resistance gene for selection of transfected cells.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

PcDNA3.1 expression plasmid PcDNA3 expression plasmid PcDNA3.1-Hygro PcDNA3.1 plasmid PcDNA3 plasmid PcDNA3.1 (þ) PcDNA3.1

2 protocols using pcdna3.1 hygro expression plasmid

Cloning strategies for PrP constructs

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Cloning strategies used to generate the cDNAs encoding WT, Δ105–125 (ΔCR), or EGFP-tagged PrP have been described elsewhere [59 (link),60 (link)]. The EGFP-PrP construct contains a monomerized version of EGFP inserted after codon 34 of mouse PrP^C. The identity of all constructs was confirmed by sequencing the entire coding region. All constructs were cloned into the pcDNA3.1(+)/hygro expression plasmid (Invitrogen).

Stincardini C., Massignan T., Biggi S., Elezgarai S.R., Sangiovanni V., Vanni I., Pancher M., Adami V., Moreno J., Stravalaci M., Maietta G., Gobbi M., Negro A., Requena J.R., Castilla J., Nonno R, & Biasini E. (2017). An antipsychotic drug exerts anti-prion effects by altering the localization of the cellular prion protein. PLoS ONE, 12(8), e0182589.

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EGFP-PrP Construct Generation

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The EGFP-PrP construct contains a monomerized version of EGFP inserted after codon 34 of mouse PrP^{57 (link)}. The identity of all constructs was confirmed by sequencing the entire coding region. All constructs were cloned into the pcDNA3.1(+)/hygro expression plasmid (Invitrogen). The Strep-FLAG pcDNA3.1(+)/G418 NEGR-1 and the anti-NEGR-1 primary antibody were kindly provided by Giovanni Piccoli (University of Trento, Italy)^{58 (link)}. All plasmids were transfected using Lipofectamine 2000 (Life Technologies), following manufacturer’s instructions.

Spagnolli G., Massignan T., Astolfi A., Biggi S., Rigoli M., Brunelli P., Libergoli M., Ianeselli A., Orioli S., Boldrini A., Terruzzi L., Bonaldo V., Maietta G., Lorenzo N.L., Fernandez L.C., Codeseira Y.B., Tosatto L., Linsenmeier L., Vignoli B., Petris G., Gasparotto D., Pennuto M., Guella G., Canossa M., Altmeppen H.C., Lolli G., Biressi S., Pastor M.M., Requena J.R., Mancini I., Barreca M.L., Faccioli P, & Biasini E. (2021). Pharmacological inactivation of the prion protein by targeting a folding intermediate. Communications Biology, 4, 62.

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