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4 protocols using sodium phosphate

1

Biologically Synthesized Gold Nanoparticles

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The Hibiscus leaves for analysis were collected from Vellore District, South India. The chlororauric acid (HAuCl4), cysteine, Sodium chloride and sodium phosphate were purchased from Hi Media, Mumbai.
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2

Biochemical Synthesis Procedures

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Salicylic acid, hydrogen peroxide, catechol, guaiacol, polyvinylpyrrolidone, tris buffer, sodium phosphate, and di-sodium phosphate were purchased from Himedia, Bangalore, India. All chemicals used were of analytical grade (≥99% purity).
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3

Antioxidant Potential Evaluation

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Butylated Hydroxytoluene (BHT), Sodium carbonate, Folin-Ciocalteu reagent used for determination of phenolic content, rutin, catechin, ascorbic acid, nitro prusside, greiss reagent used for Nitric oxide radical scavenging assay were purchased from Sigma-Aldrich (Bangalore, India). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) used for the DPPH assay, aluminum chloride, potassium acetate, Con. HCL, Nitro blue tetrazolium (NBT), Nicotinamide adenine dinucleotide (NADH), Phenazine metho sulphate (PMS), sulfuric acid, sodium phosphate and ammonium molybdate were purchased from Hi-Media Laboratories (Mumbai, India).
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4

DPPH Antioxidant Activity Assay Protocol

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1,1-Diphenyl-2-picrylhydrazyl (DPPH), ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), nitro blue tetrazolium chloride (NBT), hydrogen peroxide (H 2 O 2 ), nicotinamide adenine dinucleotide phosphate (NADPH), ribo avin, nicotinamide adenine dinucleotide (NADH), methionine, phenazine methosulfate (PMS), Triton X-100, guaiacol, glutathione, ascorbic acid, gallic acid, rutin, and sodium salicylate were provided by Sigma-Aldrich, Merck. Ferrous sulfate (FeSo 4 ), Tris HCl, sodium phosphate, ammonium molybdate, ferric chloride (FeCl 3 ), sodium carbonate, Folin-Ciocalteu reagent, sodium carbonate (Na 2 CO 3 ), ammonia solution (NH 3 ), sulfuric acid (H 2 SO 4 ), hydrochloric acid (HCl), Fehling's solutions A and B, aluminum chloride (AlCl 3 ), mercury potassium iodide, sodium hydroxide (NaOH), isoamyl alcohol, glacial acetic acid, nitric acid, methanol, chloroform, ethanol and hexane were purchased from Himedia.
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