Flojo x

Manufactured by Tree Star

The FloJo X is a high-performance flow cytometry instrument designed for accurate and efficient cell analysis. It features advanced optics, sensitive detectors, and intuitive software for streamlined data acquisition and processing.

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Lab products found in correlation

Prism 6, by GraphPad (1 mentions) Facscanto, by BD (1 mentions) Fixable viability dye, by Thermo Fisher Scientific (1 mentions) Annexin 5 staining, by BioLegend (1 mentions) Propidium iodide, by Merck Group (1 mentions)

Spelling variants of this product

FloJo (38 citations) FloJo X software (2 citations)

2 protocols using flojo x

Multiparameter Flow Cytometry Analysis of T Cell Subsets

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Single cell lymphocyte suspensions were prepared from thymi or spleens from infected animals and processed by manual disruption through a 70µM filter. 10⁶ to 10⁷ cells were stained with fixable viability dye (eBioscience, San Diego, CA), before incubation in 2.4G2 supernatant (anti-FcRII/III). Cells were stained with surface antibodies (Affymetrix, Santa Clara, CA): anti-CD3ε (145-2C11), anti-CD4 (RM4-5), anti-CD8 (53-6.7), anti-TCRβ (H57-597), anti-CD45.2 (104) CD24 (M1/69), CD8β (eBioH35-17.2), CD62L (MEL-14), CD44 (IM7), CD69 (H1.2F3), KLRG1 (2F1), IFNγ (XMG1.2), Granzyme B (GB11) and mouse TCR Vβ screening panel (BD Biosciences, San Diego, CA). Annexin V staining was done following manufacturer recommendations (Biolegend, San Diego, CA). Intracellular cytokine staining was conducted using Fixation/Permeabilization Solution Kit (BD Cytofix/Cytoperm) (Biolegend, San Diego, CA). Samples were run by flow cytometry using a FACSCanto (BD Biosciences, San Jose, CA) and analyzed using FloJo X (TreeStar, Ashland, OR). Statistical analysis was conducted in GraphPad Prism 6.01 (GraphPad Software, La Jolla, CA)

Patel S.J, & Yokoyama W.M. (2017). CD8+ T-Cells Prevent Lethality From Neonatal Murine Roseolovirus Infection. Journal of immunology (Baltimore, Md. : 1950), 199(9), 3212-3221.

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Flow Cytometric Analysis of GFP+ Parasites

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Analyses were performed on a BDFACSDiva LSRII flow cytometer in HTS mode. Cells were stained with 5 μM propidium iodide (PI, Sigma). Quadrant gates were set used PI-stained wild-type parasites (GFP-negative) and percentage of GFP+ PI- cells determined using FloJo X software (Tree Star Inc).

McCall L.I., El Aroussi A., Choi J.Y., Vieira D.F., De Muylder G., Johnston J.B., Chen S., Kellar D., Siqueira-Neto J.L., Roush W.R., Podust L.M, & McKerrow J.H. (2015). Targeting Ergosterol Biosynthesis in Leishmania donovani: Essentiality of Sterol 14alpha-demethylase. PLoS Neglected Tropical Diseases, 9(3), e0003588.

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