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A922500

Manufactured by Cayman Chemical
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The A922500 is a laboratory centrifuge designed for general purpose applications. It features a fixed-angle rotor capable of accommodating 12 tubes up to 15 mL in volume. The centrifuge can achieve a maximum speed of 4,000 rpm and a maximum relative centrifugal force (RCF) of 2,000 x g. The unit is constructed with a durable metal housing and includes safety features such as an imbalance detector and lid-lock mechanism.

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Lab products found in correlation

Bodipy tr ceramide, by Thermo Fisher Scientific (2 mentions) Cellmask deep red, by Thermo Fisher Scientific (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Myristic d27 acid, by Merck Group (1 mentions) Nile red, by Thermo Fisher Scientific (1 mentions) Fm4 64, by Thermo Fisher Scientific (1 mentions) Lysotracker green dnd 26, by Thermo Fisher Scientific (1 mentions) Er tracker red, by Thermo Fisher Scientific (1 mentions) Er tracker blue white dpx, by Thermo Fisher Scientific (1 mentions) Er tracker green, by Thermo Fisher Scientific (1 mentions) N ethylmaleimide, by Merck Group (1 mentions) Erastin, by Cayman Chemical (1 mentions) Docosahexaenoic acid d5, by Cayman Chemical (1 mentions) Bodipy 581 591 c11, by Thermo Fisher Scientific (1 mentions) Atglistatin sml1075, by Merck Group (1 mentions) Ici 118 511, by Bio-Techne (1 mentions) Bafilomycin a1 b1793, by Merck Group (1 mentions) 2 bromopalmitate, by Merck Group (1 mentions)

5 protocols using a922500

1

Lipid Imaging Probes for Cellular Analysis

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Reagents used in this study include: Arachidonic acid-d6 (Retrotope, Inc.), Eicosapentaenoic acid-d8 (Retrotope, Inc.), Docosahexaenoic acid-d10 (Retrotope, Inc.), Erastin (Cayman Chemical), Imidazole Keto Erastin (IKE) (Stockwell laboratory), RSL3 (Stockwell laboratory), FIN56 (Gift of Rachid Skouta), FINO2 (Woerpel laboratory), Brequinar (BQR) (Cayman Chemical) PF-06424439 (Cayman Chemical), A922500 (Cayman Chemical), Arachidonic acid-d11 (Cayman Chemical), Docosahexaenoic acid-d5 (Cayman Chemical), Oleic acid-d17 (Cayman Chemical), Palmitoleic acid-d13 (Cayman Chemical), Myristic acid-d27 (Sigma-Aldrich), Cholesterol-d6 (Sigma-Aldrich), Thimerosal Ready Made Solution (Sigma-Aldrich), Miltefisone (Cayman Chemical), N-ethylmaleimide (Sigma-Aldrich), LysoTracker Green DND-26 (Invitrogen), LysoTracker Red DND-99 (Invitrogen), Nile Red (Invitrogen), ERTracker Red (Invitrogen), ERTracker Green (Invitrogen), ERTracker Blue-White DPX (Invitrogen), BODIPY TR Ceramide complexed to BSA (Invitrogen), MitoTracker Red CMXRos (Invitrogen), Hoechst 33342 (Invitrogen), BODIPY 581/591 C11(Invitrogen), CellMask Deep Red (Invitrogen), and FM 4-64 (Invitrogen).
Ji X., von Rosenvinge E.C., Johnson W.W., Armstrong R.N, & Gilliland G.L. (1996). Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography.. Proceedings of the National Academy of Sciences of the United States of America, 93(16), 8208-8213.
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2

Inflammatory Response Modulation Assay

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All fatty acids and A922500 (10012708) were from Cayman. E coli LPS (L4391), (±)‐Norepinephrine (+)‐ bitartrate salt (A0937), fenoterol hydrobromide (F1016), bafilomycin A1 (B1793), and atglistatin (SML1075) were from Sigma. Interleukin‐4 (214‐14) was from Peprotech. ICI‐118,511 (0821) was from Tocris.
Petkevicius K., Bidault G., Virtue S., Jenkins B., van Dierendonck X.A., Dugourd A., Saez‐Rodriguez J., Stienstra R., Koulman A, & Vidal‐Puig A. (2021). Norepinephrine promotes triglyceride storage in macrophages via beta2‐adrenergic receptor activation. The FASEB Journal, 35(2), e21266.
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3

Lipid Metabolism Inhibitor Compounds

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VPS34 IN-1 (#17392), PIK-III (#17002), Triacsin C (#10007448), Orlistat (#10005426), TOFA (#10005263), C75 (#10005270), Etomoxir (#11969), Trimetazidine (#18165), and A-922500 (#10012708) were purchased from Cayman Chemical (Ann Arbor, Michigan, USA). Remdesivir was purchased from Target Molecule Corp. (T7766, Boston, Massachusetts, USA). 2-bromopalmitate was purchased from Sigma-Aldrich (#21604, St. Louis, MO, USA). All compounds were resuspended in dimethylsulfoxide (DMSO).
Williams C.G., Jureka A.S., Silvas J.A., Nicolini A.M., Chvatal S.A., Carlson-Stevermer J., Oki J., Holden K, & Basler C.F. (2021). Inhibitors of VPS34 and fatty-acid metabolism suppress SARS-CoV-2 replication. Cell Reports, 36(5), 109479.
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4

Fluorescent Lipid Probes for Microscopy

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BODIPY 500/510 C4,C9 (abbreviated as “C4,C9-FA”), BODIPY 500/510 C1,C12, BODIPY FL C16, BODIPY FL C5, BODIPY TR ceramide (abbreviated as “BTC”), and HCS LipidTox Red neutral lipid stain were obtained from Thermo Fisher. C11-TOPFLUOR was from Avanti Polar Lipids. A-922500 and fatty acid amide hydrolase inhibitors were obtained from Cayman Chemical. Piperazine-based monoacylglycerol lipase inhibitors (38 (link)) were a gift of Tapio Nevalainen, University of Eastern Finland. The Malaria Box (35) was a gift from the Medicines for Malaria Venture. Fatty acid-free bovine serum albumin was obtained from SigmaAldrich (product number A7511).
Dapper C., Liu J, & Klemba M. (2022). Leveraging a Fluorescent Fatty Acid Probe to Discover Cell-Permeable Inhibitors of Plasmodium falciparum Glycerolipid Biosynthesis. Microbiology Spectrum, 10(6), e02456-22.
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5

Lipopolysaccharide-induced Inflammation Assay

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Crystallized Calloselasma rhodostoma venom, lipopolysaccharides from Escherichia coli O111:B4 (LPS), Histopaque 1077, 3,30-diaminobenzidine tablets, hydrogen peroxide 30%, ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA), HEPES, Triton X-100, leupeptin, aprotinin, phenylmethylsulfonyl fluoride (PMSF), sodium orthovanadate, protease and phosphatase inhibitor cocktail, ethylenediaminetetraacetic acid disodium salt dihydrate (Na2 EDTA), bicinchoninic acid protein assay Kit (BCA), oil Red O, AACOCF3 (arachidonyl trifluoromethyl ketone), anti-mouse Fab specific-FITC and anti-β-actin were purchased from Sigma Chem. Co. (Misssouri, USA). CAY10650, A922500, prostaglandin E2 ELISA Kit, anti-COX-1 and anti-COX-2 were purchased from Cayman Chemical (Michigan, USA). Anti-cPLA2-α, anti-PTGES and anti-p-cPLA2-α were purchased from Santa Cruz Biotechnology (Texas, USA). PureLink kit, SuperScript III Reverse Transcriptase, GeneChip WT PLUS Reagent Kit and GeneChip Clariom S Array Human were purchased from Thermo Fisher Scientific (Massachusetts, USA). iTaq Universal SYBR Green Supermix were purchased from Bio-Rad (California, USA). All salts and reagents used obtained from Merck Millipore (Darmstadt, Germany) with low endotoxin or endotoxin-free grades.
Paloschi M.V., Lopes J.A., Boeno C.N., Silva M.D., Evangelista J.R., Pontes A.S., da Silva Setúbal S., Rego C.M., Néry N.M., Ferreira e Ferreira A.A., Pires W.L., Felipin K.P., Ferreira G.E., Bozza P.T, & Zuliani J.P. (2020). Cytosolic phospholipase A2-α participates in lipid body formation and PGE2 release in human neutrophils stimulated with an l-amino acid oxidase from Calloselasma rhodostoma venom. Scientific Reports, 10, 10976.
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