Nexion model 300d

Digested solutions were assayed with an ICP-MS Nexion model 300D (PerkinElmer, Inc., Shelton, CT, USA) equipped with a triple quadrupole mass detector and a reaction cell/collision device that allows operation in three modes: without reaction gas (STD); by kinetic energy discrimination (KED) with helium as the collision gas; and in reaction mode (DRC) with ammonia as the reaction gas. Both collision and reaction gases such as plasmatic argon had a purity of 99.999% and were supplied by Praxair (Madrid, Spain). Two mass flow controllers regulated gas flows. The frequency of the generator was free-swinging and worked at 40 Mhz. Three replicates were analyzed per sample. The sample quantifications were performed with indium (In) as the internal standard. The linearity of the calibration curves for In in plasma, serum, urine, platelets and erythrocytes fulfilled the accepted criteria with a value of R² higher than 0.985. The values of the standard materials of this element (10 μg/L) used for quality controls agreed with intra and inter-assay variation coefficients of less than 5%.

In order to determine the concentration of trace minerals, analyses were performed using inductively coupled plasma mass spectrometry (ICP-MS) following the method described by Maynar et al. [24 (link)].
The decomposition of the organic matrix was achieved by heating it for 10 h at 90 °C after adding 0.8 mL HNO₃ and 0.4 mL H₂O₂ to 2 mL of plasma samples. The samples were then dried at 200 °C on a hot plate. Sample reconstitution was carried out by adding 0.5 mL of nitric acid, 10 μL of indium (In) (10 mg/L) as an internal standard, and ultrapure water to complete 10 mL. Digested solutions were analyzed with an ICP-MS Nexion model 300D (PerkinElmer, Inc., Shelton, CT, USA). Three replicates were analyzed per sample. The values of the standard materials of each element (10 μg/L) used for quality controls were in accordance with intra and inter-assay coefficient variations of less than 5%.
The hormone quantification was conducted using enzyme-linked immunosorbent assay (ELISA) with an ER-500 (Sinnowa, Germany), using the commercial tests for insulin, cortisol, testosterone, and luteinizing hormone from CisRadioquímica, SA (Madrid, Spain). Hormonal analyses were performed in duplicate by the same technician. Coefficients of variation (between and within) were less than 10% for all biochemical analyses.

Digested solutions were assayed with an ICP-MS Nexion model 300D (PerkinElmer, Inc., Shelton, CT, USA) equipped with a triple quadrupole mass detector and a reaction cell/collision device that allows operation in three modes: without reaction gas (STD); by kinetic energy discrimination (KED) with helium as the collision gas; and in reaction mode (DRC) with ammonia as the reaction gas. Both collision and reaction gases such as plasmatic argon had a purity of 99.999% and were supplied by Praxair (Madrid, Spain). Two mass flow controllers regulated gas flows. The frequency of the generator was free-swinging and worked at 40 Mhz. Three replicates were analyzed per sample. The sample quantifications were performed with indium (In) as the internal standard. The values of the standard materials of each element (10 μg/L) used for quality controls were in agreement with intra and inter-assay variation coefficients of less than 5%.

Digested solutions were assayed by an ICP-MS Nexion model 300D (PerkinElmer, Inc., Shelton, CT, USA) equipped with a triple quadrupole mass detector and a reaction cell/collision device that allows operation in three modes: without reaction gas (STD); by kinetic energy discrimination (KED) with helium as the collision gas; and in reaction mode (DRC) with ammonia as the reaction gas. Both collision and reaction gases such as plasmatic argon had a purity of 99.999% and were supplied by Praxair (Madrid, Spain). Two mass flow controllers regulated gas flows. The frequency of the generator was free-swinging and worked at 40 Mhz. Three replicates were analyzed per sample. The sample quantifications were performed with indium (In) as an internal standard. The values of the standard materials of each element (10 μg/L) used for quality controls were in agreement with intro and inter-assay variation coefficients of less than 5%.

Digested solutions were assayed with an ICP-MS Nexion model 300D (PerkinElmer, Inc., Shelton, CT, USA) equipped with a triple quadrupole mass detector and a reaction cell/collision device that allows operation in three modes: without reaction gas (STD); by kinetic energy discrimination (KED) with helium as the collision gas; and in reaction mode (DRC) with ammonia as the reaction gas. Both collision and reaction gases such as plasmatic argon had a purity of 99.999% and were supplied by Praxair (Madrid, Spain). Two mass flow controllers regulated gas flows. The frequency of the generator was free-swinging and worked at 40 Mhz. Three replicates were analyzed per sample. The sample quantifications were performed with indium (In) as the internal standard. The values of the standard materials of each element (10 μg/L) used for quality controls were in agreement with intra and inter-assay variation coefficients of less than 5%.

Digested solutions were assayed with an ICP-MS Nexion model 300D (PerkinElmer, Inc., Shelton, CT, USA) equipped with a triple quadrupole mass detector and a reaction cell/collision device that allows operation in three modes: without reaction gas; by kinetic energy discrimination with helium as the collision gas; and in reaction mode with ammonia as the reaction gas. Both collision and reaction gases such as plasmatic argon had a purity of 99.999% and were supplied by Praxair (Madrid, Spain). Two mass flow controllers regulated gas flows. The frequency of the generator was free-swinging and worked at 40 Mhz. Three replicates were analyzed per sample. The sample quantifications were performed with In as the internal standard. The linearity of the calibration curves for In in plasma, serum, urine, platelets, and erythrocytes fulfilled the accepted criteria with a value of R² higher than 0.985. The values of the standard materials of this element (10 µg/L) used for quality controls agreed with intra and inter-assay variation coefficients of less than 5%.