Soybean (Glycine max L.) seeds of cultivar ‘Sowonkong’ were purchased from a local store in Deagu, Korea. The cultivar was released as a sprout cultivar in 1999 [51 ]. The mean seed weight was 12 g of 100 seeds. Persimmon fruits of cultivar ‘Sangjudungsi’ were obtained from Sangju Persimmon Experiment Station (Gyeongsangbukdo Agricultural Research and Extention Services, Sangju, Korea). The freeze-dried fruits were ground into powder using a commercial grinder (Speed Rotor Mill, Model KT-02A, Seishin, Fukuoka, Japan) and strained through a 100-mesh sieve. The following chemicals and reagents were obtained for the present study: Indolbi (INB) (Sammi Chemical Industries, Incheon, Korea); 1,1-diphenyl-2-picrylhydrazyl (DPPH), Folin-Ciocalteau-reagent, isoflavone standards (≥95% purity, Sigma-Aldrich Corporation, St. Louis, MO, USA), dimethyl sulfoxide (DMSO), and pyrogallol (Sigma-Aldrich Corporation, St. Louis, MO, USA); amino acid standards (Wako Pure Chemical Industries, Ltd., Osaka, Japan). All the other chemicals were of analytical grade.
Amino acid standards
Manufactured by Fujifilm
Sourced in United States, Japan
Amino acid standards are laboratory products used to identify and quantify amino acids in various samples. They consist of known concentrations of individual amino acids or a mixture of multiple amino acids. These standards serve as references for analytical techniques, such as chromatography and electrophoresis, to accurately detect and measure amino acid composition.
Automatically generated - may contain errors
Lab products found in correlation
Pyrogallol, by Merck Group (3 mentions)
Isoflavone standards, by Merck Group (2 mentions)
1 1 diphenyl 2 picrylhydrazyl dpph folin ciocalteau reagent, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Folin ciocalteu phenol reagent, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
L 8900, by Hitachi (1 mentions)
Whey protein, by Yuanye Bio-Technology (1 mentions)
6 protocols using amino acid standards
1
Soybean and Persimmon Compounds Analysis
2
Soybean Isoflavone Extraction Protocol
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Soybean (Glycine max L.) cv. Seoritae, a landrace with medium seed size (40 g/100 seeds), was obtained from Nonghyup (Daegu, Korea). Illite powder was obtained from Yeongdong-gun, Chungcheong-bukdo, Korea. The following chemicals and reagents were obtained for the present study: 1,1-diphenyl-2-picrylhydrazyl (DPPH), Folin–Ciocalteau reagent, isoflavone standards (≥95% purity, Sigma-Aldrich Corporation, St. Louis, MO, USA), dimethyl sulfoxide (DMSO), and pyrogallol (Sigma-Aldrich Corporation, St. Louis, MO, USA); amino acid standards (Wako Pure Chemical Industries, Ltd., Osaka, Japan). All the other chemicals were of analytical grade.
3
Antioxidant Evaluation of Soybean Sprouts and Pu-erh Tea
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Folin–Ciocalteu phenol reagent, pyrogallol, and 1,1-diphenly-2-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (Sigma-Aldrich Corp, St. Louis, MO, USA) and amino acid standards were obtained from Wako (Wako Pure Chemical Industries, Ltd., Osaka, Japan). All the chemicals used in this study were of analytical grade.
Soybean (Glycine max L.) cv. Sowonkong [54 ], obtained from the Agricultural Research and Extension Services, Gyeongsangbuk-do, Korea, was considered to produce sprouts. The mean 100-seed weight of the cultivar was 12 g. A typical wild Pu-erh tea produced in Yunnan province of China [55 ] was used in this study.
Soybean (Glycine max L.) cv. Sowonkong [54 ], obtained from the Agricultural Research and Extension Services, Gyeongsangbuk-do, Korea, was considered to produce sprouts. The mean 100-seed weight of the cultivar was 12 g. A typical wild Pu-erh tea produced in Yunnan province of China [55 ] was used in this study.
4
Amino Acid Quantification in Methanol
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Methanol was purchased from Merck Drugs & Biotechnology Ltd. (Beijing, China). HPLC-grade acetonitrile was obtained from Fisher Companies, Inc. (Fair Lawn, NJ, USA). Amino acid standards were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The filter (0.22 μm), trichloroacetic acid (TCA), HCl, sodium citrate, and other chemicals obtained from Seebio Biotech (Shanghai) Co., Ltd. (Shanghai, China) were of analytical grade.
5
Amino Acid Extraction and Quantification
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Hydrolyzed amino acid extraction and quantification were performed as described by [48 ]. Specifically, 100 mg of leaf tissue was hydrolyzed with 1 mL of 6 N HCl for 24 h at 110 °C and then vacuum filtrated at 80 °C. Thereafter, the residue was dissolved in 2 mL of deionized H2O, and the previous step was repeated twice. The resulting residue was then dissolved in 1 mL of 0.02 N HCl solution, mixed properly, and filtered through a 0.45-µm cellulose acetate membrane. The amino acid composition was determined using an automatic amino acid analyzer (L-8900 Hitachi, Tokyo, Japan). Amino acid standards (Wako Pure Chemical Industries, Ltd., Osaka, Japan) were used to quantify the amino acids in tested samples. All the assays were conducted with replication. The quantified amino acids were expressed as µg sample−1 (Table S2 ).
6
Evaluation of Whey Protein Purity
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Whey protein was purchased from Yuan Ye Bio-Technology (purity 98 %, Shanghai, China). The MRS and YPD broths were obtained from Solarbio Science (Beijing, China). Amino acid standards were bought from Wako (Wako-Shi, Japan). All other reagents were analytical grade and purchased from Aladdin (Shanghai, China).
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