Log In Sign up
The largest database of trusted experimental protocols

Procartaplex

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States, United Kingdom, Austria, Germany, Italy

ProcartaPlex is a multiplex immunoassay platform that enables the simultaneous detection and quantification of multiple analytes from a single sample. The core function of ProcartaPlex is to provide a flexible and high-throughput solution for measuring a wide range of protein targets in a variety of sample types.

Automatically generated - may contain errors

Lab products found in correlation

Bio plex 200 system, by Bio-Rad (12 mentions) Bio plex manager software, by Bio-Rad (7 mentions) Luminex 200, by DiaSorin (6 mentions) Bio plex 200, by Bio-Rad (6 mentions) Flexmap 3d, by DiaSorin (5 mentions) Facscalibur, by BD (4 mentions) Elisa, by R&D Systems (4 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (3 mentions) Bio plex 200 multiplex suspension array system, by Bio-Rad (3 mentions) Bio plex 5, by Bio-Rad (3 mentions) Dade innovin reagent, by Siemens (3 mentions) Dade actin fs activated ptt reagent, by Siemens (3 mentions) Flexset cytometric bead array, by BD (3 mentions) Procartaplex analyst 1, by Thermo Fisher Scientific (3 mentions) Magpix, by DiaSorin (3 mentions) Magpix instrument, by DiaSorin (3 mentions) Procartaplex analyst software, by Thermo Fisher Scientific (3 mentions) Chi3l1 elisa, by Bio-Techne (2 mentions) Bep 2000 elisa analyser, by Siemens (2 mentions) Apoptag fluorescein in situ apoptosis detection kit, by Merck Group (2 mentions)

167 protocols using procartaplex

1

Cytokine Profiling of Parasite-DC Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen supernatants derived from parasite-FL-DC co-culture experiments were tested for the presence of IFN-γ, IL-1β, IL-2; IL-4, IL-5, IL-9, IL-12p70, IL-13, IL-17a, IL-18, IL-22, IL-23, IL-27, GM-CSF, IP-10, eotaxin, and MIP-1α by ProcartaPlex (eBioscience) multiplex as per manufacturer's instructions. Presence of MIP-2, IL-6, IL-10, GRO-α, MCP-1, MCP-3, MIP-1β, RANTES, and TNF-α were assayed for by both FlowCytomix (eBioScience) and ProcartaPlex. Expression of IFN-α and IFN-β was measured by a separate multiplex (ProcartaPlex).
Yap X.Z., Lundie R.J., Feng G., Pooley J., Beeson J.G, & O'Keeffe M. (2019). Different Life Cycle Stages of Plasmodium falciparum Induce Contrasting Responses in Dendritic Cells. Frontiers in Immunology, 10, 32.
+ Open protocol
+ Expand
2

Multiplex Cytokine and Chemokine Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokines and chemokines in serum were measured by the ProcartaPlex assay (mouse ProcartaPlex panel 1A, Invitrogen), performed on a Luminex 200 machine (Luminex). A panel of 36 cytokines and chemokines were analyzed: CXCL1 (C-X-C motif chemokine 1), CXCL2, CXCL5, CXCL10, CCL2 (C-C motif ligand 2), CCL3, CCL4, CCL5, CCL7, Eotaxin, IFN-α (interferon-α), IFN-γ, IL-1α (interlukin-1α), IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-15/IL-15R, IL-17A, IL-18, IL-22, IL-23, IL-27, IL-28, IL-31, G-CSF (granulocyte colony-stimulating factor), GM–CSF (granulocyte-macrophage colony-stimulating factor), M-CSF (macrophage colony-stimulating factor), LIF (leukemia inhibitory factor), and TNF-α (tumor necrosis factor-α), according to the manufacturer’s instructions. Cytokine concentrations were determined from the appropriate standard curves of known concentrations of recombinant mouse cytokines and chemokines to convert fluorescence units to concentrations (pg/mL). Each sample was run in duplicate, and the mean of the duplicates was used to calculate the measured concentration.
Marié I.J., Brambilla L., Azzouz D., Chen Z., Baracho G.V., Arnett A., Li H.S., Liu W., Cimmino L., Chattopadhyay P., Silverman G., Watowich S.S., Khor B, & Levy D.E. (2021). Tonic interferon restricts pathogenic IL-17-driven inflammatory disease via balancing the microbiome. eLife, 10, e68371.
+ Open protocol
+ Expand
3

Multiplex Cytokine Profiling After GS-9620 Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
After 48 h in culture, supernatants were collected from each well and stored at −80°C until they were ready for analysis. The supernatants were inactivated using 10% Triton X and probed, using the ProcartaPlex multiplex immunoassay (Affymetrix eBioscience) according to the manufacturer's instructions, for 29 cytokines and chemokines: APO-FAS, BAFF, granzyme B, GRO-α, IFN-α, IFN-γ, IFN-ω, IL-1β, IL-1RA, IL-10, IL-12p70, IL-15, IL-2, IL-21, IL-23, IL-27, IL-29, IL-6, IL-8, IL-10, I-TAC, MCP-1, MIP-1α, MIP-1β, MMP-1, RANTES, SDF-1α, TNF-α, and TRAIL. Samples were then read on a Luminex 200 (Luminex) platform and analyzed using Bio-plex Manager software (Bio-Rad). Previous analyses were also done with a multiplex that included IL-4, IL-5, IL-7, IL-13, IL-17A, IL-18, IL-31, and sCD-40L, but they were found to be unaffected by GS-9620 treatment.
Tsai A., Irrinki A., Kaur J., Cihlar T., Kukolj G., Sloan D.D, & Murry J.P. (2017). Toll-Like Receptor 7 Agonist GS-9620 Induces HIV Expression and HIV-Specific Immunity in Cells from HIV-Infected Individuals on Suppressive Antiretroviral Therapy. Journal of Virology, 91(8), e02166-16.
+ Open protocol
+ Expand
4

Multiplex Cytokine Analysis in BALF

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokine levels in BALF were measured with ProcartaPlex multiplex immunoassay (Affymetrix, eBioscience) on a Luminex 200 system. The human cytokine panel included: interferon (IFN)‐γ, interleukin (IL)‐1β, IL‐2, IL‐4, IL‐5, IL‐6, IL‐12p70, IL‐13, IL‐18, tumor necrosis factor (TNF)‐α, IFN‐α, IL‐1α, IL‐1RA, IL‐7, IL‐15, IL‐31, granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), TNF‐β, IL‐9, IL‐10, IL‐17A, IL‐21, IL‐22, IL‐23, IL‐27, Eotaxin, GRO‐α (also CXCL1), MCP‐1 (also CCL2), IL‐8 (also CXCL8), IP‐10 (also CXCL10), RANTES (also CCL5), macrophage inflammatory protein (MIP)‐1α (also CCL3), MIP‐1β (also CCL4), and SDF‐1α (CXCL12a). The results are reported in pg/mL.
Si X., Zheng X., Tian X., Wang H., Xu Y., Zhao J., Chen M., Zhong W., Wang M., Zhang L, & Zhang X. (2023). Analysis of cytokines in bronchoalveolar lavage fluid in patients with checkpoint inhibitor pneumonitis and pulmonary infection: A case–control study. Thoracic Cancer, 14(21), 2038-2044.
+ Open protocol
+ Expand
5

Mouse Cytokine/Chemokine Multiplex Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse serum cytokine and chemokine levels were measured with Mouse Cytokine/Chemokine bead immunoassay kit, ProcartaPlex, 26Plex from Affymetrix eBioscience according to the manufacturer’s specifications using the Bio-Plex 20 (Bio-Rad) and analyzed with five parametric curve fitting.
Buerger C., Shirsath N., Lang V., Berard A., Diehl S., Kaufmann R., Boehncke W.H, & Wolf P. (2017). Inflammation dependent mTORC1 signaling interferes with the switch from keratinocyte proliferation to differentiation. PLoS ONE, 12(7), e0180853.
+ Open protocol
+ Expand
6

Cytokine Quantification in Infected Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Interleukin 1β (IL-1β), IFN-γ, and TNF-α levels were analyzed in serum as well as spleen and liver homogenates from infected mice using a custom-made ProcartaPlex immunoassay panel (Affymetrix, eBioscience) according to the manufacturer’s protocol.
Dragset M.S., Ioerger T.R., Loevenich M., Haug M., Sivakumar N., Marstad A., Cardona P.J., Klinkenberg G., Rubin E.J., Steigedal M, & Flo T.H. (2019). Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence. mSystems, 4(6), e00402-19.
+ Open protocol
+ Expand
7

Multiplex Immunoassay for Serum Ig and Cytokines

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Ig concentrations were measured in the mice serum by Luminex following the manufacturer’s protocol (Affymetrix E-bioscience; Human Isotyping procartaplex). Cytokines were quantified in the mice serum using the human Premixed Multi-Analyte kit from Bio-Techne with Luminex-based technology as specified by manufacturer. The following cytokines were analyzed: IFN-γ, TNF-α and Granzyme B23 (link).
Samson C., Thiolat A., Moktefi A., Cohen J.L., Pilon C, & Grimbert P. (2023). Belatacept inhibit human B cell germinal center development in immunodeficient mice. Scientific Reports, 13, 13816.
+ Open protocol
+ Expand
8

Cytokine and Chemokine Profiling in CTP Recipients

Check if the same lab product or an alternative is used in the 5 most similar protocols
A series of cytokines (IL-6, IL8, INF-y, TNF-α), chemokines (CCL2/MCP-1, CCL3/MIP-α, CCL4/MIP-β, CCL5/RANTES, CCL11/Eotaxin, CXCL10/IP-10, CXCL11/I-TAC, MIG, CXCL13/BLC, CXCL12/SDF-1α) and the neurotrophin BDNF have been evaluated in the sera and CSF of the CTP recipients at baseline and at different time-points following transplantation. These analytes were evaluated using a customized multiplex assay (Procartaplex, Multiplex Immunoassay, Affymetrix, Milan, Italy) by Luminex platform (Labscan100, Onelambda, Milan, Italy) in accordance with the manufacturer’s instructions. YKL-40 levels were determined for all the CSF samples using the Microvue CHI3L1 ELISA kit (Quidel Corporation, San Diego, CA) according to the manufacturer’s protocol. Absorbance was measured using a microplate reader (Bio-Rad).
Aron Badin R., Bugi A., Williams S., Vadori M., Michael M., Jan C., Nassi A., Lecourtois S., Blancher A., Cozzi E., Hantraye P, & Perrier A.L. (2019). MHC matching fails to prevent long-term rejection of iPSC-derived neurons in non-human primates. Nature Communications, 10, 4357.
+ Open protocol
+ Expand
9

Cardiac-derived Monocyte Activation by EVs

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cardiac-derived monocytes were fluorescence-activated cell–sorted from LVs, 24 hours after the onset of MI. Fluorescence-activated cell–sorted CD45+/CD11b+/Ly6G−/Ly6C+ monocytes were counted, seeded at a density of 4.5×105 cells per well, and cultured in complete RPMI (Roswell Park Memorial Institute)-1640 medium (1% exosome-depleted serum; 1% β-mercaptoethanol and penicillin streptomycin). Cells were then stimulated for 24 hours with lEVs or sEVs derived from MI mice at a ratio of 105 lEVs per cell or 103 sEVs per cell to reproduce local concentrations of infiltrating monocytes and those of lEVs and sEVs in the infarcted heart 24 hours postligation. NaCl 0.09% was used as control vehicle. TNFα (tumor necrosis factor alpha), CCL (chemokine ligand) 2, CCL7, IL (interleukin)-4, IL-5, IL-6, IL-10, IL-12, IL-13, and IFNγ (interferon gamma) levels were quantified using ProcartaPlex multiplex immunoassays panels (Affymetrix, eBioscience, France).10 (link),11 (link) Concentrations were calculated from a control standard curve.
Loyer X., Zlatanova I., Devue C., Yin M., Howangyin K.Y., Klaihmon P., Guerin C.L., Kheloufi M., Vilar J., Zannis K., Fleischmann B.K., Hwang D.W., Park J., Lee H., Menasché P., Silvestre J.S, & Boulanger C.M. (2018). Intra-Cardiac Release of Extracellular Vesicles Shapes Inflammation Following Myocardial Infarction. Circulation Research, 123(1), 100-106.
+ Open protocol
+ Expand
10

Multiplex Cytokine Profiling of Stimulated PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
After fresh PBMC isolation and stimulation for 5 days with anti-CD3/anti-CD28, 0.5 mL culture supernatants were taken from unstimulated and stimulated wells, centrifuged, and stored at −20 °C until they were used in multiplex bead assay. The Milliplex® MAP kit (EMD Millipore, Burlington, MA, USA) was used to detect GM-CSF, IFN-γ, IL-2, IL-7, IL-10, IL-12p70, IL-17, and IL-23. The ProcartaPlex® (Affymetrix eBioscience) kit was used to detect IL-1β, IL-6, IL-15, and IL-18. An ELISA kit (BioLegend) was used to detect free active TGF-β1. Samples were processed according to manufacturer’s protocols. Multiplex samples were run on a Bio-Rad (Bio-Plex® 200 System) machine. ELISA samples were run on Benchmark plus microplate reader (Bio-Rad).
Aram J., Frakich N., Morandi E., Alrouji M., Samaraweera A., Onion D., Spendlove I., Colombo S.L., Tanasescu R., Gran B, & Constantinescu C.S. (2020). Increased IL-2 and Reduced TGF-β Upon T-Cell Stimulation are Associated with GM-CSF Upregulation in Multiple Immune Cell Types in Multiple Sclerosis. Biomedicines, 8(7), 226.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!