Beclin 1 sc 11 427

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Beclin-1 (sc-11,427) is a protein involved in the regulation of autophagy, a cellular process that recycles damaged or unwanted components. This product is a research tool used to study the role of Beclin-1 in various biological processes.

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Beclin-1 (108 citations)

6 protocols using beclin 1 sc 11 427

Resveratrol-Induced Apoptosis Signaling Pathway

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A caspase-3 assay kit ((Sigma SCP0084)), anti-β-actin (A2547), anti-rabbit-secondary antibody (Sigma A0545), and anti-mouse-secondary antibody (Sigma A9044) were purchased from Sigma (St. Louis, MO, USA). Resveratrol was kindly given by Chongqing Kerui Nanhai Pharmaceutical Company and a 500 mM stock solution was made in DMSO (0.1% v/v final concentration) stored at − 80 °C. Antibody against phospho-LKB-1 (sc-271,934), Bcl-2 (sc-492), AMPK (sc-19,128), phospho-AMPK (sc-101,630), Bax (sc-6236), and Beclin-1 (sc-11,427) were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Compound C (sc-200,689) and Z-DEVD-FMK (sc-311,558) were also purchased from Santa Cruz Biotechnology Inc. Antibody against cleaved caspase-3 was purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against Bid (bs-1153R) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China). RPMI 1640 medium was obtained from Gibco and fetal bovine serum was purchased from Shanghai Excell Biological Technology Co., Ltd. (Shanghai, China). Annexin V, propidium iodide (PI) and the caspase-3 inhibitor Ac-DEVD-CHO (C1206) were obtained from the Beyotime Institute of Biotechnology (Jiangsu, China).

Fan Y., Chiu J.F., Liu J., Deng Y., Xu C., Zhang J, & Li G. (2018). Resveratrol induces autophagy-dependent apoptosis in HL-60 cells. BMC Cancer, 18, 581.

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Gambogenic Acid: Potential Therapeutic Insights

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Gambogenic acid (GNA) (Figure 1A), isolated from Gamboge, was supplied by Prof. Xiaoshan Wang's lab. The GNA purity was 99%, which was determined by HPLC-DAD. MTT, monodansylcadaverine (MDC), propidium iodide (PI), Annexin V, acridine orange and Hoechst 33342 were purchased from Sigma. Rapamycin and trehalose were kind gifts from Prof. Guanghui Wang. MitoTracker Red, LysoTracker Red and LysoSensor Green DND-189 were obtained from Invitrogen. LC3 antibodies were purchased from Novus (NB100-2220), p62 antibodies were from Biomol (PW9860), P70S6K (1494-1), p-P70S6K (3204-1) and p-p38 (1229-1) antibodies were from Epitomics. GAPDH antibody was purchased from CHEMICON (MAB374). GFP (sc-9996), caspase-3 (sc-7148), p53 (sc-6243), Bax (sc-7480), Beclin 1 (sc-11427), and Bcl-2 (sc-7382) antibodies were purchased from Santa Cruz Biotechnology. The reagents were dissolved in phosphate-buffered saline (PBS), except GNA and rapamycin, which were prepared in DMSO.

Mei W., Dong C., Hui C., Bin L., Fenggen Y., Jingjing S., Cheng P., Meiling S., Yawen H., Xiaoshan W., Guanghui W., Zhiwu C, & Qinglin L. (2014). Gambogenic Acid Kills Lung Cancer Cells through Aberrant Autophagy. PLoS ONE, 9(1), e83604.

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Detailed Autophagy and Apoptosis Assays

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The antibodies and apoptosis detection kit used were as follows: LC3 (3868), Cell Signaling Technology (Danvers, MA, USA); p62/SQSTM1 (ab56416) and Pax1 (ab95227), Abcam (Cambridge, UK); beclin1 (sc-11427), brachyury (sc-17743), and CD24 (sc-11406), Santa Cruz Biotechnology (Santa Cruz, CA, USA); α-tubulin (T9026), Sigma-Aldrich (St. Louis, MO, USA); TUNEL (11684795910), Roche Diagnostics (Mannheim, Germany).
The reagents used for cell culture were as follows: Dulbecco’s modified Eagle’s medium (DMEM) (D5796) and fetal bovine serum (FBS) (F2442), Sigma-Aldrich; penicillin/streptomycin (26253-84), Nakarai Tesque (Kyoto, Japan); collagenase type 2 (LS004176), Worthington Biochemical (Lakewood, NJ, USA).
The reagents used for Western blotting and immunostaining were obtained from Thermo Fisher Scientific (Waltham, MA, USA), Nakarai Tesque, Bio Craft (Tokyo, Japan), GE Healthcare (Chicago, IL, USA), Nichirei Biosciences (Tokyo, Japan), and Dako (Glostrup, Denmark).
The other reagents used were obtained from Wako Pure Chemical Industries (Osaka, Japan).

Yurube T., Hirata H., Ito M., Terashima Y., Kakiuchi Y., Kuroda R, & Kakutani K. (2021). Involvement of Autophagy in Rat Tail Static Compression-Induced Intervertebral Disc Degeneration and Notochordal Cell Disappearance. International Journal of Molecular Sciences, 22(11), 5648.

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Autophagy Markers in Neurodegeneration

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Streptozotocin (STZ, S0130), LC3 (for immunoblotting, #L8918), and p62 (#P0067) were purchased from Sigma (St. Louis, MO, USA). LC3 for immunostaining (#PM036) was purchased from MBL international (Woburn, MA, USA). Cathepsin D (sc-377124), ATP6E (sc-20946) and Beclin-1 (sc-11427) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). p-AKT (#2965), Rab7 (#9367) and beta-actin (#4970) were procured from Cell signaling (Danvers, MA, USA). Phospho-CREB (#05-807) and TrkB (#07-225) were purchased from Millipore (Billerica, MA, USA). BDNF (AB1779SP) and calbindin (AB1778) were purchased from Chemicon (Billerica, MA, USA).

Bak D.H., Zhang E., Yi M.H., Kim D.K., Lim K., Kim J.J, & Kim D.W. (2015). High ω3-polyunsaturated fatty acids in fat-1 mice prevent streptozotocin-induced Purkinje cell degeneration through BDNF-mediated autophagy. Scientific Reports, 5, 15465.

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Antibody Sourcing and Characterization

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The following antibodies were purchased from Cell Signaling Technology: LC3 (#4108 and #12741), β-ACTIN (#4967), SIRT3 (#5490), phospho-ULK1 (#5869), and VDAC (#4661). Antibodies were purchased from Santa Cruz Biotechnology: PARKIN (sc-32282), myc (sc-40), ha (sc-805), ULK1 (sc-390904), Beclin1 (sc-11427), and UBI (sc-8017 HRP). The following antibodies were purchased from Sigma-Aldrich: flag (F3165). The following antibodies were purchased from Abcam: SQSTM1/p62 (ab56416). The following antibodies were purchased from Invitrogen: Alexa Fluor 488-labeled anti-rabbit IgG (A11034), Alexa Fluor 568-labeled anti-mouse IgG (A11031), Alexa Fluor 568-labeled anti-rat IgG (A11077), Alexa Fluor 647-labeled anti-mouse IgG (A21237), and OXPHOS (#45–7999). A rabbit polyclonal antibody against mouse AIBP was a kind gift from Dr. Longhou Fang (Houston Methodist Research Institute). A rabbit polyclonal antibody against LDLR (10785–1-AP) was purchased from Proteintech. Antibodies against CD68 (#137001) and CD36 (#102612) were purchased from BioLegend. An antibody against CD16/CD32 (FcRr blocker, #553141) and CD31 (#562939) were purchased from BD Bioscience and Bafilomycin A1 (#196000) was purchased from Calbiochem.

Choi S.H., Agatisa-Boyle C., Gonen A., Kim A., Kim J., Alekseeva E., Tsimikas S, & Miller Y.I. (2020). Intracellular AIBP regulates oxidized LDL-induced mitophagy in macrophages. Arteriosclerosis, thrombosis, and vascular biology, 41(2), e82-e96.

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Autophagy Modulation Protein Markers

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H 2 S donor, NaHS (Sodium hydrosulfide, 161527) was purchased from Sigma-Aldrich.
Anti-β-actin (sc-47778, 1:1,000), Beclin-1 (sc-11427, 1:1,000) antibodies were purchased from Santa Cruz. Anti-LC3B/MAP1LC3B (NB600-1384, 1:1,000) antibody was purchased from Novus Biologicals. Anti-p62 (PM-045, 1:1,000) antibody and positive control for anti-LC3 antibody (PM036-PN) were purchased from MBL.
Peroxidase-conjugated affinipure goat anti-rabbit IgG (H+L) (ZB-2301, 1:2500) was purchased from ZSGB-BIO. Goat-anti-mouse IgG (IS001, 1:10,000) was purchased from M&C Gene Technology. 3-MA (369172) and rapamycin (948477) were purchased

Shui M., Liu X., Zhu Y, & Wang Y. (2016). Exogenous hydrogen sulfide attenuates cerebral ischemia-reperfusion injury by inhibiting autophagy in mice. Canadian journal of physiology and pharmacology, 94(11).

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