Hepg2

The HCC cell lines (Huh7, Hep3B, HepG2, and SNU-449) were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea) (Supplementary Materials).

HCC cell lines HepG2 and Huh7 were purchased from Korean Cell Line Bank (Seoul, Korea). Human hepatocellular carcinoma cell line HepG2 and Huh7 cells were cultured in MEM and RPMI-1640, respectively. And all media contained with 10% heat-inactivated FBS, penicillin and streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) to maintain. The cells were cultured at 37 °C in a humidified atmosphere with 5% CO2. BET inhibitor, JQ1 and OTX-015 were purchased from Tocris Bioscience (Minneapolis, MN, USA). JQ1 and OTX-015 were dissolved in dimethyl sulfoxide (DMSO) at a stock concentration of 10 mM and stored at -20 °C. The cells were treated with various concentrations of JQ1 or OTX-015 for different lengths of time.

Hep3B, HepG2, and Huh7 cells were purchased from the Korean Cell Line Bank (Seoul, Korea) and cultured in Dulbecco’s minimum essential medium (Corning, NY, USA), supplemented with 10% fetal bovine serum (FBS; Corning) and 1% penicillin–streptomycin (P/S; Corning). Human NK-92 cells were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in α-minimum essential medium (Corning), which was composed of 12.5% FBS (Corning), 12.5% horse serum (HS; Sigma-Aldrich, St. Louis, MO, USA), 1% P/S (Corning), 0.2 mM Myo-inositol (Sigma-Aldrich), 0.1 mM 2-mercaptoethanol (Sigma-Aldrich), 0.02 mM folic acid (Sigma-Aldrich), 10 ng/mL Interleukin (IL)-2 (Miltenyi Biotec, Bergisch Gladbach, Germany), and 20 ng/mL IL-15 (Miltenyi Biotec) activating cytokines. Normal human liver THLE-2 cells were obtained from Dr. KP Kim (Asan Medical Center, Seoul, Korea) and were cultured in bronchial epithelial cell growth medium (BEGM Bullet Kit; Lonza, Basel, Switzerland) as per the manufacturer’s protocol. All cells were cultured at 37 °C with 5% CO₂.

Human colorectal cancer cell lines (HCT116, SW480 and LoVo), human breast cancer cell lines (MCF-7 and MDA-MB231) and human hepatocellular carcinoma (HepG-2) were purchased from Korean Cell Line Bank (Seoul, Korea) and grown in DMEM/F-12 supplemented with 10% fatal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin. The cells were maintained at 37°C under a humidified atmosphere of 5% CO₂. The extracts of ginger leaf (GL) were dissolved in dimethyl sulfoxide (DMSO) and treated to cells. DMSO was used as a vehicle and the final DMSO concentration did not exceed 0.1% (v/v).