Samples from different organs of the cotton plants, including cotyledon, hypocotyl, petiole, leaf and stem of one or many different gland accessions, served as the source of total RNA, were immediately frozen in liquid nitrogen and stored at − 80 °C. Total RNAs were isolated from 100 mg of leaf ground with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO., LTD) according to the manufacturer’s instructions. The quantity and purity of RNAs were assessed according an absorbance ratio of OD 260/280 (1.9–2.1) using a NanoDrop OneC Microvolume UV-Vis Spectrophotometer with Wi-Fi (Thermo Fisher Scientific Inc., Waltham, MA, USA) ultraviolet spectrophotometer, and was confirmed using 1.0% (w/v) denatured formaldehyde agarose gel electrophoresis to investigate its integrality. First strand cDNA was synthesized by the PrimmeScript™ II 1st strand cDNA Synthesis Kit (TaKaRa Bio, Dalian, China) following the manufacturer’s protocol of Reverse Transcription System.
Nanodrop onec microvolume uv vis spectrophotometer with wi fi
Manufactured by Thermo Fisher Scientific
Sourced in United States
The NanoDrop OneC Microvolume UV-Vis Spectrophotometer with Wi-Fi is a compact and versatile laboratory instrument designed for the analysis of small volume samples. It utilizes a patented sample retention system to measure the absorbance of nucleic acids, proteins, and other biomolecules in sample volumes as low as 0.5 μL. The instrument features a built-in Wi-Fi connection for data transfer and remote access capabilities.
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2 protocols using nanodrop onec microvolume uv vis spectrophotometer with wi fi
1
Total RNA Extraction from Cotton Plants
2
Isolation of High-Quality Total RNA
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Samples from different tissues of the cotton plants, including cotyledon, hypocotyl, petiole, true leaf and stem of one or many different gland accessions, served as the source of total RNA were immediately frozen in liquid nitrogen and stored at -80°C. For each sample, total RNAs were isolated from 100 mg of leaf ground with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO., LTD) according to the manufacturer's instructions. The quantity and purity of RNAs were assessed according an absorbance ratio of OD 260/280 (1.9-2.1) using a NanoDrop One C Microvolume UV-Vis Spectrophotometer with Wi-Fi (Thermo Fisher Scientific Inc., Waltham, MA, USA) ultraviolet spectrophotometer, and was confirmed using 1.0% (w/v) denatured formaldehyde agarose gel electrophoresis to investigate its integrality.
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