To determine the size of as-synthesized nanocrystals, a dilute dispersion of nanocrystals in hexane was drop cast onto an ultrathin carbon film on lacey carbon support, 400 mesh copper TEM grid (Ted Pella) and dried in a fume hood. Images were collected on a Gemini Ultra-55 Analytical Field Emission Scanning Electron Microscope (Zeiss) in dark-field transmission mode under 30 kV accelerating voltage or on a JEOL 2100-F in HAADF mode under 200 kV accelerating voltage. Diameters for 100 random nanoparticles were manually designated in ImageJ and a distribution was plotted. To determine the size of aqueous nanoparticles, dispersions were diluted to 20 nM in 100 mM HEPES, pH 7.8 for PAOA-encapsulated nanoparticles, and in 100 mM HEPES, pH 7.0, for PMAO-encapsulated and SpyCatcher-functionalized nanoparticles. The dispersions were sonicated for 30 min prior to measurement. Diameters were measured by dynamic light scattering using a Malvern Zetasizer with typical count rates of 150 kilocounts per second. Data were collected for 60 sec each in 5 separate runs and fits using Malvern Zetasizer software to a volume-weighted size distribution of hydrodynamic diameter.
Malvern zetasizer
Manufactured by Malvern Panalytical
Sourced in United Kingdom, United States, Germany
The Malvern Zetasizer is an analytical instrument designed to measure the size, size distribution, and zeta potential of particles and molecules in a sample. It utilizes the principle of dynamic light scattering to determine the hydrodynamic size of particles, and laser Doppler velocimetry to measure the zeta potential. The Zetasizer provides accurate and reliable data on the physical characteristics of a wide range of materials, including polymers, proteins, and colloids.
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Lab products found in correlation
Nano z, by Malvern Panalytical (5 mentions)
Avanti mini extruder, by Avanti Polar Lipids (4 mentions)
Nanosight ns300, by Malvern Panalytical (2 mentions)
Malvern zetasizer nano z, by Malvern Panalytical (2 mentions)
Tyloxapol, by Merck Group (2 mentions)
Tyloxapol, by Malvern Panalytical (2 mentions)
Dts1070, by Malvern Panalytical (2 mentions)
Nano zs zen 3600, by Malvern Panalytical (2 mentions)
Nanosep tubes, by Pall Corporation (2 mentions)
Cell enhances capillary, by Malvern Panalytical (2 mentions)
2010f fastem, by JEOL (2 mentions)
Dppe peg 2000, by Avanti Polar Lipids (2 mentions)
Spd 20a ultraviolet visible uv vis light detector, by Shimadzu (2 mentions)
Em cpd300 scanning electron microscope, by Leica (2 mentions)
Matlab r2016b, by MathWorks (2 mentions)
Fast camera, by Photron (2 mentions)
Fei tecnai g2 twin, by Thermo Fisher Scientific (2 mentions)
Te2000 u, by Nikon (2 mentions)
Supra 55vp, by Zeiss (2 mentions)
Nanodrop 1000 spectrophotometer, by Thermo Fisher Scientific (2 mentions)
207 protocols using malvern zetasizer
1
Nanoparticle Size Determination by TEM and DLS
2
Liposome Size and Zeta Potential Analysis
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The particle size and size distribution of liposomes were determined by Malvern zetasizer based on laser light scattering principle. A Malvern laser light scattering zetasizer equipped with an argon laser was utilized for evaluating the particle size and size distribution. Light scattering was monitored at 90° angle and at 25 °C. The mean droplet size was calculated from intensity, volume and bimodal distribution assuming spherical particles. The zeta potential is an indication of the stability of the colloidal systems and indicates charge present in the colloidal systems. Zeta potential of formulations was determined using Malvern zetasizer. Samples were placed in clear disposable zeta cells and results were recorded.
3
Characterization of Functionalized Gold Nanoparticles
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T‐AuNPs and S‐AuNPs were characterized with respect to size (DLS, nm), surface charge (zeta potential, mV), stability (λmax), and loading of the fluorescent ligands. For DLS measurements, 1 nmol/L T‐AuNP or S‐AuNP was analyzed using the Malvern Zetasizer (Malvern Instruments Ltd, Worcestershire, UK). Data are presented as mean hydrodynamic diameter ± standard deviation. The surface charge of the T‐AuNP and S‐AuNP (concentration: 1 nmol/L) was quantified using the Malvern Zetasizer (Malvern Instruments Ltd). Data are presented as mean zeta potential ± standard deviation. Stability of the T‐AuNP and S‐AuNP was measured using UV‐Vis spectroscopy with reference made to citrate‐stabilized AuNPs (λmax = 520 nm). Peptide loading onto the AuNPs was quantified using a fluorescent microplate reader whereby surface ligands labeled with a molecular fluorophore were liberated from the AuNPs using potassium cyanide to dissolve the AuNP core. After digestion, the fluorescence was then read at 565 nm on the BioTek Synergy 2 plate reader (BioTek, Winooski, VT) and the number of Alexa Fluor 546 molecules per nanoparticle was quantified using a standard curve of each peptide‐AF546‐PEG ligand. Data are presented as the mean number of ligands per nanoparticle ± standard deviation.
4
Characterization of Electroactive Ionic Liquids
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The particle size and distribution were determined by quasielastic laser light scattering with a Malvern Zetasizer (Malvern Instruments, United Kingdom) at 25 °C. About 0.2 mL of EIL suspension were diluted in 2.5 mL water immediately after preparation. Each experiment was repeated three times. Zeta potential was measured using a Malvern Zetasizer (Malvern Instruments). Zeta limits ranged from -150 to 150 V. The magnetic properties of EILs were determined through using a vibrating samples magnetometer (LDJ9600-1, LDJ Electronics Inc., United States).
5
Zeta Potential Analysis of Microemulsions
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Zeta potential analysis of the prepared ME was assessed using Malvern Zetasizer. Samples were diluted with deionized water before each measuring. One milliliter of the diluted samples was placed in a folded capillary cells supported with platinum electrodes. The values were calculated using the Dispersion Technology software built into the Malvern Zetasizer.28 (link)
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Zeta potential analysis of the prepared ME was assessed using Malvern Zetasizer. Samples were diluted with deionized water before each measuring. One milliliter of the diluted samples was placed in a folded capillary cells supported with platinum electrodes. The values were calculated using the Dispersion Technology software built into the Malvern Zetasizer.28 (link)
6
Particle Size and Zeta Potential Analysis
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The particle size and the size distribution of the formulations were determined by Malvern zetasizer based on the laser light scattering principle. A Malvern laser light-scattering zetasizer equipped with an argon laser was utilized for evaluating the particle size and the size distribution. Light scattering was monitored at 90 angle and at 25 C. The mean droplet size was ascertained from force, volume, and bimodal distribution expecting circular particles. The zeta potential is a notation of the strength of the dispersion systems and demonstrates charge present on the dispersion systems. Zeta potential of formulations was measured using Malvern zetasizer.
7
Characterization of Polymer-Coated Nanoparticles
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Polymer coated NPs (MYTS) were characterized by Jeol JEM 2100Plus (Jeol, Tokyo, Japan) electron microscope (TEM) equipped with a 9 MP complementary metal oxide superconductor (CMOS) Gatan Rio9 digital camera (Gatan, Inc. Pleasanton, CA, USA). Dynamic Light Scattering (DLS) and ζ-potential measurements of MYTS, MYTS-EDBE and MYTS-PEG (0.01–0.05 mg/mL NPs, at pH 7.2) were carried out using a Malvern Zetasizer (Malvern Instruments, Malvern, UK).
8
Characterization of Lipid Nanoparticle Size
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The LNPs in RNAse-free water (5 times dilution) were sized in terms of Z-average size (nm) using dynamic light scattering (DLS) at 25 °C with a fixed angle of 90° using the Malvern Zetasizer® (Nano ZS, Malvern Instrument, Worchestershire, UK). The polydispersity index (PdI) and zeta potential (mV) were determined with the same instrument. All of the experiments were measured in triplicate (n = 3) at 25 °C. Mean size and LNPs concentration were also measured by NTA using the NanoSight NS300 (Malvern Instrument, Worchestershire, UK).
9
Characterization of Amphiphilic Beclomethasone Dipropionate-Loaded Lipid Nanoparticles
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The size distributions and zeta potentials were measured using a dynamic light scattering (DLS) technique on Malvern Zetasizer (Malvern Instruments Ltd., UK). Ultraviolet–visible (UV–vis) absorption spectra were measured with an UV–vis spectrometer (Q-5000, Quawell, America). The amounts of BDP in AB-LNPs were analyzed using UV–vis spectroscopy after the dissolution of AB-LNPs in DMSO by measuring the absorbance at a wavelength of 600 nm. The loading efficiency (LE, %) was determined and calculated using the following formula:
10
Characterization of Nanoparticle Properties
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