Standards of anthocyanins (cyanidin-3-sambubioside, cyanidin-3,5-diglucoside, cyanidin-3-glucoside) were purchased in Extrasynthese (Genay, France). Trolox, gallic acid, ABTS, 2 M Folin–Ciocalteu reagent (all Sigma Aldrich, St. Louis, MO, USA), potassium persulfate (Laborchemie, Apolda, Germany), potassium chloride, sodium acetate, and sodium carbonate (all Lach-ner, Neratovice, Czech Republic) were used for spectrophotometric measurements. Methanol, formic acid (both Sigma Aldrich), and deionized water (Milli-Q purification system, Merck Millipore, Darmstadt, Germany) were used for extraction and separation.
Potassium chloride (kcl)
Manufactured by Lach-Ner
Sourced in Germany, Czechia
Potassium chloride (KCl) is a chemical compound that consists of the elements potassium and chlorine. It is a white, crystalline solid that is commonly used as a laboratory reagent and in various industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Lach-Ner (5 mentions)
Kh2po4, by Lach-Ner (3 mentions)
Na2hpo4, by Merck Group (3 mentions)
Hydrochloric acid (hcl), by Lach-Ner (2 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (2 mentions)
K2s2o8, by Lach-Ner (2 mentions)
Sodium dodecyl sulphate (sds), by Thermo Fisher Scientific (2 mentions)
Dinitrophenylhidrazine, by Thermo Fisher Scientific (2 mentions)
Guanidine hydrochloride for molecular biology, by Merck Group (2 mentions)
Ethyl acetate, by ITW Reagents (2 mentions)
2m folin ciocalteu reagent, by Merck Group (1 mentions)
Milli q purification system, by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (1 mentions)
Sodium carbonate, by Lach-Ner (1 mentions)
Formic acid, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Sodium acetate, by Lach-Ner (1 mentions)
Itaconic acid, by Thermo Fisher Scientific (1 mentions)
N isopropylacrylamide nipaam, by Thermo Fisher Scientific (1 mentions)
10 protocols using potassium chloride (kcl)
1
Quantification of Anthocyanin Standards
2
Enzyme-Responsive Hydrogel Synthesis
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Both monomers, itaconic acid (IA) and N-isopropylacrylamide (NiPAAm), were purchased from Acros Organics (Belgium). N,N′-methylenebisacrylamide (MBA) from Serva Feinbiochemica (Germany) was used as the crosslinking agent. The initiator and accelerator, potassium persulfate (PPS) and potassium pyrosulfate (PPyroS), were obtained from Merck & Co., Inc. (Germany), and Acros, respectively. Lipase from Candida rugosa (CRL) was kindly obtained from Sigma-Aldrich Chemie Gmbh (Germany). NiPAAm was recrystallized from benzene/n-hexane mixture (35/75) before its utilization. Other materials were used as received, without purification. Different pH value aqueous media were prepared using hydrochloric acid (LaChema, Czech Republic), potassium chloride (Zorka Šabac, Serbia), sodium dihydrogen phosphate dihydrate, and disodium hydrogen phosphate dodecahydrate (Lach-Ner, s.r.o., Czech Republic). Molar concentrations of all buffer solutions used were 0.2 M. Distilled water was used for all syntheses and the preparation of the buffer solutions.
3
Potassium Standard Curve Preparation
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Calcium carbonate (CaCO3) was purchased from PanReac Quimica SLU (Barcelona, Spain) and potassium chloride (KCl) was procured from Lachner (Neratovice, Czech Republik). All chemicals and solvents used were of analytical grade.
Preparation of basic standard solution for potassium 0.5 g of potassium chloride was previously dried for 2 hours at 105 °C and then dissolved in 1 liter of distilled water. Five standard solutions for the potassium calibration curve were prepared in the concentration range from 0-20 mg/l. The standard solutions consist of basic standard solution 0.5 g/l and 10 ml of AL working solution.
Preparation of basic standard solution for potassium 0.5 g of potassium chloride was previously dried for 2 hours at 105 °C and then dissolved in 1 liter of distilled water. Five standard solutions for the potassium calibration curve were prepared in the concentration range from 0-20 mg/l. The standard solutions consist of basic standard solution 0.5 g/l and 10 ml of AL working solution.
4
Extraction and Quantification of Plant Alkaloids
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Alkaloids were extracted from plant material in the Department of Biochemistry, Faculty of Science, Masaryk University (Brno, Czech Republic). The oligonucleotides used in this work (Table 1 ) were purchased as dry samples from Thermo Fisher Scientific (Waltham, MA, USA) at HPLC grade. Other reagents, such as KH2PO4, KCl, NaOH, KOH, and CH3COONa (all p.a. grade), were purchased from Lach-Ner (Neratovice, Czech Republic).
5
Synthesis and Characterization of 5-FU Nanocarriers
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Chemicals
used for the synthesis of the different samples synthesized herein
are anhydrous AlCl3 (Sigma-Aldrich, 98%) and MgCl2 (Alfa Aesar, 99%) as metal precursors, NaOH (VWR Chemicals, 99.1%)
as precipitating agent, 5-FU (Sigma, >99%) as a model anticancer
drug,
phosphate buffer (Na2HPO4 Sigma-Aldrich >99%;
NaH2PO4 Honeywell >99%), distillated water
(Thermo
Scientific), EtOH (Honeywell, 99.8%), NaCl (Lachner, 99.97%), KCl
(Lachner, 100%), MgCl2·6H2O (Roth, 99%),
and CaCl2 (Lachner, 96.3%).
used for the synthesis of the different samples synthesized herein
are anhydrous AlCl3 (Sigma-Aldrich, 98%) and MgCl2 (Alfa Aesar, 99%) as metal precursors, NaOH (VWR Chemicals, 99.1%)
as precipitating agent, 5-FU (Sigma, >99%) as a model anticancer
drug,
phosphate buffer (Na2HPO4 Sigma-Aldrich >99%;
NaH2PO4 Honeywell >99%), distillated water
(Thermo
Scientific), EtOH (Honeywell, 99.8%), NaCl (Lachner, 99.97%), KCl
(Lachner, 100%), MgCl2·6H2O (Roth, 99%),
and CaCl2 (Lachner, 96.3%).
6
Antioxidant Capacity Evaluation of Hops
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6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, 97%) and n-alkane standard solution C7-C30 (1000 μg/mL each component in hexane) were purchased from Sigma-Aldrich Chemie (Taufkirchen, Germany); 2-(3-hydroxy-6-oxo-xanthen-9-yl)benzoic acid, fluorescein (FL) and 2,2′-azobis-2-methyl-propanimidamide dihydrochloride (AAPH) were from Fluka Analytical (Bornem, Belgium); NaCl, KCl, KH2PO4 and K2S2O8 were from Lach-Ner (Brno, Czech Republic); Na2HPO4 was from Merck KGaA (Darmstadt, Germany); carbon dioxide and nitrogen gases (99.9%) were from Gaschema (Jonava, Lithuania). International Calibration Extract 4 (ICE-4), containing α- and β-acids (10.98% of cohumulone; 31.60% of humulone+adhumulone; 13.02% colupulone; 13.52% lupulone+adlupulone), was obtained from Labor Veritas AG (Zürich, Switzerland). Divinylbenzene/Carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fibers and 20 mL SPME vials were purchased from Supelco (Bellefonte, PA, USA). All solvents were of analytical and HPLC-grade.
7
Antioxidant Capacity Assays Protocol
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2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulphonic acid), (ABTS); 2,2-diphenyl-1-picrylhydrazyl hydrate free radical (DPPH•, 95%); gallic acid (GA, 3,4,5-trihydroxybenzoic acid, 99%); 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, 97%); and Na2CO3 were purchased from Sigma-Aldrich (Steinheim, Germany). Folin–Ciocalteu’s phenol reagent (2 M); 2-(3-hydroxy-6-oxo-xanthen-9-yl)benzoic acid (fluorescein); and 2,2′-azobis-2-methyl-propanimidamide dihydrochloride (AAPH) were from Fluka Analytical (Bornem, Belgium). KCl, NaCl, K2S2O8, and KH2PO4 were from Lach-Ner (Brno, Czech Republic). Na2HPO4 and isoamyl alcohol (a mixture of isomers) were from Merck KGaA (Darmstadt, Germany). Agricultural origin ethanol (96.3%) was from Stumbras (Kaunas, Lithuania). Liquid nitrogen was from AGA SIA (Riga, Latvia). CO2 and N2 gases (99.9%) were from Gaschema (Jonava region, Lithuania). Perchloric acid; 2-thiobarbituric acid; 1,1,3,3 tetraethoxypropane; butylated hydroxytoluene; sulfuric acid; and boric acid were purchased from Sigma-Aldrich Chemie (Steinheim, Germany). Sodium hydroxide (NaOH, 50%) was from Ingle AS (Ingliste, Estonia), and Kjeltabs FOSS Analytical A/S was from Oridor Eesti OÜ (Tartu, Estonia).
8
Trout Feed Protein Composition Analysis
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NaCl, KCl and trichlorоacetic acid (TCA) were purchased from Lachner (Neratowitz, Czech Republic). Sodium dodecyl sulphate was purchased from Thermo Fisher (Kandel, Germany). Dinitrophenylhidrazine was purchased from Acros Organics (Geel, Belgium). Ethyl acetate was obtained from Panreac (Barcelona, Spain). Guanidine hydrochloride for molecular biology was purchased from Sigma Aldrich (St. Louis, USA) and ethanol was obtained from Reahem (Srbobran, Serbia). Extruded rainbow trout feed was purchased from a local fish feed market and used in this research. The particular fish feed manufacturer was chosen due to the various protein sources, that the rainbow trout feed contained. Fish meal, soy concentrate, wheat meal, haemoglobin, corn and gluten were the protein sources in the extruded rainbow trout feed.
9
Establishing Methods for Serum Analysis
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Reagents. -Ultrapure water (electrolytic conductivity 0.055 μS cm -1 ) was used throughout. Hydrochloric acid 35 % (Lach-Ner, Czech Republic), glycerol (Gram-mol, Croatia), NaHCO 3 (Kemika, Croatia), Na 2 HPO 4 × 2H 2 O (Merck, Germany), KCl (Lach-Ner), MgCl 2 × 6H 2 O (Kemika), CaCl 2 × 2H 2 O (Merck Alkaloid, Macedonia), NaCl (Zorka, Serbia) and HNO 3 65 % (Kemika) were all of pro analysi grade.
Human albumin 20 % and human intravenous immunoglobulin 5 % were both from the Institute of Immunology (Zagreb, Croatia).
Serum samples. -All experiments were approved by the Ethical Committ ees of the Srebrnjak Children's Hospital, Zagreb, Croatia, and Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia. To establish new methods, the pool of remnant children's sera was used. All sera samples were obtained from the Children's Hospital Srebrnjak, Zagreb, Croatia, in 2006 and 2007, and were stored at -18 °C. Before analysis, the serum sample was thawed overnight and mixed for 10 min.
HCl-acidifi ed serum pool was prepared by adding 3 mol dm -3 HCl into the serum pool to a fi nal dilution ratio 1:5 (AS/5) or 1:10 (AS/10).
Human albumin 20 % and human intravenous immunoglobulin 5 % were both from the Institute of Immunology (Zagreb, Croatia).
Serum samples. -All experiments were approved by the Ethical Committ ees of the Srebrnjak Children's Hospital, Zagreb, Croatia, and Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia. To establish new methods, the pool of remnant children's sera was used. All sera samples were obtained from the Children's Hospital Srebrnjak, Zagreb, Croatia, in 2006 and 2007, and were stored at -18 °C. Before analysis, the serum sample was thawed overnight and mixed for 10 min.
HCl-acidifi ed serum pool was prepared by adding 3 mol dm -3 HCl into the serum pool to a fi nal dilution ratio 1:5 (AS/5) or 1:10 (AS/10).
10
Trout Feed Protein Composition Analysis
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NaCl, KCl and trichlorоacetic acid (TCA) were purchased from Lachner (Neratowitz, Czech Republic). Sodium dodecyl sulphate was purchased from Thermo Fisher (Kandel, Germany). Dinitrophenylhidrazine was purchased from Acros Organics (Geel, Belgium). Ethyl acetate was obtained from Panreac (Barcelona, Spain). Guanidine hydrochloride for molecular biology was purchased from Sigma Aldrich (St. Louis, USA) and ethanol was obtained from Reahem (Srbobran, Serbia). Extruded rainbow trout feed was purchased from a local fish feed market and used in this research. The particular fish feed manufacturer was chosen due to the various protein sources, that the rainbow trout feed contained. Fish meal, soy concentrate, wheat meal, haemoglobin, corn and gluten were the protein sources in the extruded rainbow trout feed.
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