Total sod assay kit

The supernatant of normal saline homogenates was used to measure the activities of the important antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) and catalase (CAT), as well as glutathione (GSH) and malonaldehyde (MDA) levels in the liver, according to the manufacturer's instructions [total SOD assay kit (hydroxylamine method), GSH-PX assay kit (colorimetric method), GST assay kit (colorimetric method), GSH assay kit (colorimetric method), CAT assay kit (ultraviolet) and MDA assay kit (TBA method); Nanjing Jiancheng Bioengineering Institute, Nanjing, China]

Melatonin and palmitic acid (PA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Recombinant human CTRP3 (rhCTRP3) was purchased from Aviscera Bioscience (Santa Clara, CA, USA). Anti-B-cell lymphoma-2 (Bcl-2, 1:1000) was obtained from Cell Signaling Technology (Danvers, Massachusetts). Anti-Nrf2 (1:1000), anti-heme oxygenase-1 (HO-1, 1:1000), anti-superoxide dismutase 2 (SOD2, 1:1000), anti-tumour necrosis factor-α (TNF-α, 1:200 for staining), anti-CD68 (1:200 for staining) and anti-CTRP3 (1:500 for western blotting, 1:200 for staining) were purchased from Abcam (Cambridge, UK). Anti-adiponectin (1:200 for staining) was purchased from Proteintech (Chicago, IL, USA). The secondary antibody used in the study was purchased from LI-COR Biosciences (1:10,000 dilution). Dihydroethidium (DHE) was purchased from Invitrogen (Carlsbad, CA, USA). 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), total SOD assay kit, catalase (CAT) assay kit, and glutathione (GSH) assay kit were purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The mini-osmotic pump was purchased from Alzet (DURECT Corp, Cupertino, CA). Cell counting kit-8 (CCK-8) was obtained from Dōjindo Laboratories (Kumamoto, Japan). All other chemicals were of analytical grade.