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7 aminoactinomycin d 7 aad expression

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7-Aminoactinomycin D (7-AAD) is a fluorescent dye used in flow cytometry to detect and quantify cell death or apoptosis. It binds to DNA and emits fluorescence upon excitation, allowing the identification of cells with compromised cell membranes, a characteristic of late-stage apoptosis or necrosis.

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Lab products found in correlation

Annex 5 binding, by BD (1 mentions) Annexin 5 binding, by BD (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Promega (1 mentions)

Close spelling variants of this product

7-aminoactinomycin D (7-AAD) 7-aminoactinomycin (7-AAD, 7-aminoactinomycin D 7-AAD

2 protocols using 7 aminoactinomycin d 7 aad expression

1

Measuring Apoptosis by Flow Cytometry

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Apoptosis was quantified using flow cytometry as detailed in our previous publication [37 (link)], for Annex V binding (BD Biosciences) and 7-Aminoactinomycin D (7-AAD) expression (eBioscience). Caspase 3 activity was evaluated by Western blotting.
Wang L., Howell M.E., Sparks-Wallace A., Hawkins C., Nicksic C.A., Kohne C., Hall K.H., Moorman J.P., Yao Z.Q, & Ning S. (2019). p62-mediated Selective autophagy endows virus-transformed cells with insusceptibility to DNA damage under oxidative stress. PLoS Pathogens, 15(4), e1007541.
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2

Quantification of Apoptosis and Proliferation

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Apoptosis was quantified using flow cytometry as detailed in our previous publication (37 (link)), for annexin V binding (BD Biosciences) and 7-aminoactinomycin D (7-AAD) expression (eBioscience). Caspase 3 activity was evaluated by immunoblotting. An MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide] proliferation assay was carried out following the manufacturer’s instructions (Promega).
Wang L., Howell M.E., Sparks-Wallace A., Zhao J., Hensley C.R., Nicksic C.A., Horne S.R., Mohr K.B., Moorman J.P., Yao Z.Q, & Ning S. (2021). The Ubiquitin Sensor and Adaptor Protein p62 Mediates Signal Transduction of a Viral Oncogenic Pathway. mBio, 12(5), e01097-21.
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