Goat anti mouse igg fitc

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Goat anti-mouse IgG-FITC is a secondary antibody conjugated with the fluorescent dye Fluorescein Isothiocyanate (FITC). It is used to detect and visualize mouse immunoglobulin G (IgG) in various immunoassays and cell-based applications.

Automatically generated - may contain errors

Lab products found in correlation

32 protocols using goat anti mouse igg fitc

Immunofluorescent Labeling of Lung Claudin-18 and p-SPAK

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

We performed immunofluorescence staining by a published procedure [27 (link)]. Lung sections were treated with primary rabbit polyclonal antibody, claudin-18 (diluted 1:200, Proteintech, IL, USA), and phosphorylated-SPAK (p-SPAK) (diluted 1:100, OriGene, MD, USA) for immunofluorescent labeling. The secondary antibody was goat anti-mouse IgG-FITC (diluted 1:200, Santa Cruz Biotechnology, USA) and Rhodamine (TRITC) AffiniPure Goat Anti-Rabbit IgG (diluted 1:200, Jackson ImmunoResearch Inc. PA, USA). The slides were mounted with VECTASHIELD Antifade Mounting Medium (Vector Laboratories, Inc. CA, USA) and DAPI. Images were obtained using a DeltaVision system (Applied Precision) comprising a wide-field inverted microscope (model IX-71; Olympus) with × 60/1.42 Plan Apo N or × 100/1.40 Super-Plan APO objectives.

Shen C.H., Lin J.Y., Lu C.Y., Yang S.S., Peng C.K, & Huang K.L. (2021). SPAK-p38 MAPK signal pathway modulates claudin-18 and barrier function of alveolar epithelium after hyperoxic exposure. BMC Pulmonary Medicine, 21, 58.

+ Open protocol

+ Expand

Oxidative Stress Signaling Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rotenone, Antimycin A, goat anti-mouse IgG-HRP, and goat anti-mouse IgG-FITC were bought from Santa Cruz (Santa Cruz Biotechnology, Inc., CA, USA). Anti-Peroxiredoxin-3 antibody was purchased from Abcam (Abcam, Cambridge, MA, USA). Superoxide dismutase (SOD) was bought from Sigma (Sigma-Aldrich, MO, USA). Rat cleaved caspases 3 and 9 ELISA kits were purchased from Chenglin (Chenglin Biotechnology, Beijing, China). TUNEL assay kit was purchased from Boster (Boster, Wuhan, China). FRTL, diethyldithiocarbamate (DETC), MitoSOX Red, MTT, LDH kit, and thyroid stimulating hormone (TSH) were purchased as previously described [2 (link)].

Wang L., Duan Q., Wang T., Ahmed M., Zhang N., Li Y., Li L, & Yao X. (2015). Mitochondrial Respiratory Chain Inhibitors Involved in ROS Production Induced by Acute High Concentrations of Iodide and the Effects of SOD as a Protective Factor. Oxidative Medicine and Cellular Longevity, 2015, 217670.

+ Open protocol

+ Expand

Cell Proliferation Measurement by BrdU and PI

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell proliferation was measured by labeling cells with bromodeoxyuridine (BrdU) and propidium iodide (PI) prior to flow cytometry. BrdU-positive cells and PI staining were used to identify cells in S phase and expression of total DNA [25 (link), 26 (link)]. Cells were treated with SH003 for 48 h and labeled with 10 μM BrdU (Sigma-Aldrich) for 1 h before harvesting. Cells were then trypsinized and fixed in 70% ethanol on ice for 20 min. Next, cells were incubated with 2 M HCl/0.5% Tween-20/phosphate-buffered saline (PBS) for 30 min at room temperature. After washing with 1% bovine serum albumin (BSA) in PBS, cells were stained with anti-BrdU antibody (1:50; Santa Cruz, CA, USA) in buffer (0.5% Tween-20/1% BSA in PBS) for 30 min at room temperature. Cells were washed and then incubated for 30 min at room temperature with goat anti-mouse IgG-FITC (1:100; Santa Cruz). Washed cells were resuspended in PI for 30 min on ice. Cell proliferation was analyzed by FACSCalibur using CellQuest Pro software.

Choi Y.J., Choi Y.K., Lee K.M., Cho S.G., Kang S.Y, & Ko S.G. (2016). SH003 induces apoptosis of DU145 prostate cancer cells by inhibiting ERK-involved pathway. BMC Complementary and Alternative Medicine, 16, 507.

+ Open protocol

+ Expand

Immunofluorescence Staining of Cardiomyocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

After the cells were allowed to differentiate for 15 days, they were washed with PBS, fixed in 4 % paraformaldehyde for 15 min, permeabilized with 0.5 % Triton-X 100 for 10 min and blocked with 5 % bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA) at 37 °C for 30 min. Subsequently, the cells were first incubated with mouse monoclonal anti-cardiac troponin T antibody (cTnT, 1:100; Abcam, Cambridge, MA, USA) for 90 min, followed by staining with goat anti-mouse IgG-FITC (1:400; Santa Cruz Biotechnology, Dallas, TX, USA) for 60 min at room temperature. After the cells were washed three times with PBS to remove the excess staining, they were incubated with rabbit polyclonal anti-myosin light chain (MLC2a, 1:100, Santa Cruz Biotechnology Inc) primary antibody for 90 min at room temperature. This incubation was followed by incubation with goat anti-rabbit IgG-TR (1:400; Santa Cruz) for 60 min at room temperature. After the cells were rinsed with PBS, the nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:1000; Sigma, St. Louis, MO, USA) for 5 min. The samples were finally imaged using a fluorescence microscope (IX71, Olympus, Tokyo, Japan).

Chen Y., Zeng D., Ding L., Li X.L., Liu X.T., Li W.J., Wei T., Yan S., Xie J.H., Wei L, & Zheng Q.S. (2015). Three-dimensional poly-(ε-caprolactone) nanofibrous scaffolds directly promote the cardiomyocyte differentiation of murine-induced pluripotent stem cells through Wnt/β-catenin signaling. BMC Cell Biology, 16, 22.

+ Open protocol

+ Expand

Immunohistochemistry and Immunofluorescence Assays

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemistry (IHC) and immunofluorescence (IF) were performed as previously described.^{10 (link)} The following primary antibodies were used: rabbit anti-Runx1 (Abcam, ab23980), mouse anti-Col2α1 (Santa Cruz, sc-52658), rabbit anti-MMP13 (Abcam, ab39012), rabbit anti-ADAMTS5 (Santa Cruz, sc-83186), rabbit anti-Yap (Cell Signaling Technology, 14074 S), p-Smad2/3 (Cell Signaling Technology, 8828 S), and mouse anti-active-β-catenin (Millipore, 05–665). The secondary antibodies were goat anti-rabbit IgG-FITC, goat anti-rabbit IgG-TRITC, goat anti-mouse IgG-FITC and goat anti-mouse IgG-TRITC from Santa Cruz. Images were taken by a Leica DMLB microscope and a Leica D3000 fluorescence microscope. ImageJ software was used to perform counts for the quantification of IHC or IF staining.

Zhang Y., Zuo T., McVicar A., Yang H.L., Li Y.P, & Chen W. (2022). Runx1 is a key regulator of articular cartilage homeostasis by orchestrating YAP, TGFβ, and Wnt signaling in articular cartilage formation and osteoarthritis. Bone Research, 10, 63.

+ Open protocol

+ Expand

Characterization of PLA-Collagen Biomaterials

Check if the same lab product or an alternative is used in the 5 most similar protocols

Poly-l,d-lactic acid (96% l/4% d) (PLA) was purchased from Purac BV (Gorinchem, the Netherlands). Rat tail collagen type I was obtained from BD Biosciences. Mouse anti-human collagen type I and III IgG primary antibodies and goat anti-mouse IgG FITC were obtained from Santa Cruz Biotechnology. Primary human coronary artery smooth muscle cells (HCASMCs), human umbilical vein endothelial cells (HUVECs) pooled, medium 200, medium 231, low-serum growth supplement (LSGS), smooth muscle growth supplement (SMGS), cell tracker (CMAC), and 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester, mixed isomers (CFSE) were all obtained from GIBCO, Life Technologies. Fura-2/AM and prostaglandin I₂ were both purchased from Cambridge Biosciences. Thrombin was obtained from Merck Chemicals. Chloroform, dimethyl formamide (DMF), rhodamine B, Sudan black B, Tween 20, fibronectin from bovine plasma, 3,3′-dihexyloxacarbocyanine iodide (DiOC₆), and apyrase were all purchased from Sigma Aldrich.

Musa F.I., Harper A.G, & Yang Y. (2016). A Real-Time Monitoring System to Assess the Platelet Aggregatory Capacity of Components of a Tissue-Engineered Blood Vessel Wall. Tissue Engineering. Part C, Methods, 22(7), 691-699.

+ Open protocol

+ Expand

Flow Cytometric Analysis of RHAMM and CD44 in LAD2 Mast Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

LAD2 MCs were incubated for 1 h in normoxia (21% O₂) or hypoxia (1% O₂) and fixed under the same conditions for 5 min in 500 μL of flow cytometry fixation buffer (R&D Systems). After fixation, MCs were washed in FACS buffer containing 1 × PBS, 1% BSA, and 0.1% sodium azide and were subsequently resuspended in 100 μL of this buffer. Next, MCs were incubated for 30 min with primary nonspecific antibody (mouse IgG1 isotype control, Novus Biologicals) at a 1:1000 dilution, with primary antibody against the RHAMM receptor (RHAMM/CD168 antibody, Novus Biologicals) at a 1:500 dilution followed by incubation in the dark for 30 min at 4 °C with fluorescence-labeled secondary antibody (goat anti-mouse IgG-FITC, Santa Cruz Biotechnology) at a 1:400 dilution, with primary nonspecific antibody (purified rat IgG2a κ isotype control, BD Pharmingen) at a 1:500 dilution, and with primary antibody against the CD44 receptor (CD44 antibody, Novus Biologicals) at a 1:500 dilution, followed by fluorescence-labeled secondary antibody (goat anti-rat Ig-FITC, BD Pharmingen) at a 1:500 dilution. Finally, MCs were washed with FACS buffer and resuspended in 1 mL of FACS buffer. Flow cytometry analysis was performed using a BD LSR Fortessa™ (BD Biosciences).

Pastwińska J., Walczak-Drzewiecka A., Kozłowska E., Harunari E., Ratajewski M, & Dastych J. (2021). Hypoxia modulates human mast cell adhesion to hyaluronic acid. Immunologic Research, 70(2), 152-160.

+ Open protocol

+ Expand

Immunofluorescence and Alkaline Phosphatase Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescence (IF) was performed after
fixation of the cultured cells in 4% paraformaldehyde
for 20 minutes followed by permeabilization with
0.2% Triton X-100 (Merk, USA) for 30 minutes.
The cells were subsequently blocked in phosphate-
buffered saline (PBS) supplemented with 10%
secondary antibodies host serum for 1 hour. The
blocked cells were incubated overnight at 4°C with
mouse anti-Oct4 (Santa Cruz, USA, sc5279), mouse
anti-SSEA-1 (R&D, MAB2155) and goat anti-Nanog
(Santa Cruz, USA, sc30329). The cells were washed
three times with PBS and subsequently incubated with
the following secondary antibodies goat anti-mouse
IgG-FITC (Santa Cruz, USA, sc2010), Alexa Fluor
568 goat anti-mouse (Invitrogen, USA, A21043),
and Alexa Fluor 568 donkey anti-goat (Invitrogen,
USA, A11057). The cells were stained with 1 µg/
ml DAPI for 10 minutes in the dark and after three
PBS washes, we used an Olympus fluorescent
microscope (Olympus, Japan) to visualize the cells.
Alkaline phosphatase (ALP) staining was performed
according to the manufacturer’s instructions using an
Alkaline Phosphatase Detection Kit.

Taleahmad S., Hassani S.N., Salekdeh G.H, & Baharvand H. (2018). Metabolic Signature of Pluripotent Stem Cells. Cell Journal (Yakhteh), 20(3), 388-395.

+ Open protocol

+ Expand

Immunofluorescence Imaging of CacyBP Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were grown on poly-L-lysine-coated coverslips, fixed with 4% paraformaldehyde, and permeabilized with 0.25% Triton X-100. Cells were then blocked with 1% bovine serum albumin, and probed with anti-CacyBP MAb (1:10) for overnight at 4°C. After washing, cells were incubated with goat anti-mouse IgG-FITC (1:50; Santa Cruz Biotechnology, Santa Cruz, USA), mounted on glass slides,and imaged by a confocal laser microscope (Bio-Rad Laboratories, Inc., USA). As a negative control, cells were incubated with preimmune serum.

Zhai H., Shi Y., Chen X., Wang J., Lu Y., Zhang F., Liu Z., Lei T, & Fan D. (2017). CacyBP/SIP promotes the proliferation of colon cancer cells. PLoS ONE, 12(2), e0169959.

+ Open protocol

+ Expand

HHV-8 Infection Visualization by Immunofluorescence

Check if the same lab product or an alternative is used in the 5 most similar protocols

Infection of target cells was visualized by IFA using antibodies against the HHV-8 proteins ORF59, K8.1 and LANA-1 (Advanced Biologics Inc, Columbia, MD). Initially, infected cells were stained with antibodies against all three proteins. These results demonstrated that ORF59 expression was always present in infected cells and therefore, in later experiments, we only used antibodies against ORF59 to demonstrate viral infection. For immunofluorescence, cells were fixed in 1% paraformaldehyde, permeabilized in 0.1% Triton-X100 in PBS and dried to poly-L-lysine coated slides. Wells were blocked in 10% goat serum in PBS for 30 min at 37°C and subsequently incubated with the primary antibody at 37°C for 1 hr. Wells were washed in PBS and incubated with goat anti-mouse-IgG-FITC (ORF59 and K8.1, Santa Cruz Biotechnology, Santa Cruz, CA) or goat anti-rat-IgG-FITC (LANA-1, Dako, Glostrup, Denmark) at 37°C for 1 hr. K562 cells expressing DC-SIGN mutants were confirmed for DC-SIGN expression by IFA using a polyclonal anti-DC-SIGN antibody (Calbiochem, Gibbstown, NJ) and goat-anti-rabbit IgG-FITC (Santa Cruz Biotechnology, Santa Cruz, CA) and/or monoclonal anti-DC-SIGN (R&D Systems, Minneapolis, MN) and goat-anti-mouse IgG-FITC (Santa Cruz Biotechnology, Santa Cruz, CA) in a similar fashion.

Hensler H.R., Tomaszewski M.J., Rappocciolo G., Rinaldo C.R, & Jenkins F.J. (2014). Human herpesvirus 8 glycoprotein B binds the entry receptor DC-SIGN. Virus research, 190, 97-103.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!